Labshake search
Citations for GE Life Sciences :
1201 - 1250 of 1931 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
-
bioRxiv - Immunology 2024Quote: ... filtered culture supernatant was purified with a 5 mL HisTrap FF Crude column (GE Healthcare Life Sciences) previously equilibrated in Buffer A (20 mM NaH2PO4 ...
-
bioRxiv - Bioengineering 2023Quote: ... The diluted sample was loaded onto the pre-equilibrated HiTrap Q FF 5 mL column (GE Healthcare) at a flow rate of 2.5 mL/min ...
-
Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...
-
bioRxiv - Immunology 2023Quote: ... the cortex was homogenized and resuspended with 5 ml 70% Percoll Plus (GE Healthcare, 17-5445-02) and 5 ml 37% Percoll Plus placed on top ...
-
bioRxiv - Plant Biology 2023Quote: ... Fluorescence scanning of acrylamide gels was performed on an Amersham Typhoon 5 scanner (GE Healthcare Life Sciences) using excitation and emission wavelengths of ex488/em520 and ex635/em670 for Cy2 and Cy5 scanning ...
-
bioRxiv - Bioengineering 2023Quote: ... A single-use 10 L Cell bag™ bioreactor (working volume: 0.5-5 L; GE Healthcare, USA) was placed on the rocker ...
-
bioRxiv - Microbiology 2022Quote: ... the supernatant was loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 40 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
-
bioRxiv - Bioengineering 2023Quote: ... Three consecutives’ batches were performed in 5 L bioreactor using ReadyToProcess WAVE 25 system (GE Healthcare, USA). A single-use 10 L Cell bag™ bioreactor (working volume ...
-
Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare) ...
-
bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
-
bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... to a volume of 500 μL and exchanged with a NAP-5 column (GE Healthcare Life Sciences) to a buffer consisting of 20 mM Tris (pH 7.5) ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
-
bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... We then loaded the cleaved samples onto a 5 mL HiTrap Q-sepharose FF column (GE Healthcare) and eluted the material with a gradient of 0%-100% buffer (20 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2024Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
-
bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to a 5 ml HiTrap TALON crude column (GE Healthcare, Little Chalfont, UK), washed with lysis buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... The eluted proteins were loaded onto a HiTrap Q HP 5 ml anion exchange column (GE Healthcare) pre-equilibrated with QA buffer ...
-
bioRxiv - Microbiology 2024Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
-
bioRxiv - Biophysics 2024Quote: ... Rep ΔCys A97CA473C was purified by chromatography on a 5 ml His-trap FF column (GE Healthcare) using a 100 ml gradient of 5 mM to 1 M imidazole in buffer A ...
-
bioRxiv - Molecular Biology 2024Quote: ... The supernatant was separated on a 5 ml HiTrap TALON crude column (GE Healthcare, Little Chalfont, UK), using washing in lysis buffer supplemented with 10 mM imidazole ...
-
bioRxiv - Biochemistry 2024Quote: ... The sample was loaded on 3 × 5 mL HiTrap™ DEAE Sepharose FF (GE Healthcare, Munich, Germany) equilibrated with the same buffer ...
-
bioRxiv - Biophysics 2024Quote: ... The clarified lysate was loaded onto a 5 mL nickel-nitrilotriacetic acid (Ni-NTA) resin (GE Healthcare) pre-equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... +4°C) and supernatant was loaded into a 5 mL Nickel sepharose column (HisTrap HP, GE Healthcare). The loaded column was first washed ...
-
bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were washed with 1x TTBS and placed in a 1:25000 secondary HRP-conjugated IgG anti-rabbit or anti-mouse solution (GE Healthcare®, Sweden) according to the primary antibody datasheet specifications ...
-
bioRxiv - Microbiology 2020Quote: ... The tissue solution was passed through two cell sieves (100µm then 70µm) and then layered with Ficoll®-Paque Premium (GE Healthcare, Hatfield, United Kingdom). Density gradient centrifugation was carried out at 400g for 30 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were mixed with 10μl of 10μg/μl solution of Sera-Mag SpeedBeadsTM hydrophilic and hydrophobic magnetics beads (GE healthcare, ref 45152105050250 and 65152105050250) with a beads to sample ratio of 10:1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
-
bioRxiv - Biophysics 2022Quote: ... was titrated into an A1 protein solution (22 μM except 50 μM with A1+OGly+N+C) in a MicroCal iTC200 (GE Healthcare Life Sciences). A priming injection of 0.4 μL (not included in data analysis ...
-
bioRxiv - Microbiology 2022Quote: ... the membrane was incubated with a self-made developing solution and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ (37).
-
bioRxiv - Pathology 2020Quote: ... The tissue solution was passed through two cell sieves (100μm then 70μm) and then layered with Ficoll®-Paque Premium (GE Healthcare, Hatfield, United Kingdom). Density gradient centrifugation was carried out at 400g for 30 min on dissociated tissue and on whole blood ...
-
bioRxiv - Bioengineering 2020Quote: ... The pellet was re-suspended in 4 ml PBS prior to layering over 4 ml of Ficoll solution (GE Healthcare life sciences, UK) in a 15 ml centrifuge tube and centrifuged at 1400 x g for 45 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... Released mesh probes were rinsed with deionized (DI) water three times and transferred to sterile 1× PBS solution (HyClone, GE Healthcare Life Sciences) before surface modification or implantation.
-
bioRxiv - Biophysics 2024Quote: ... His-tag containing proteases and uncleaved nanobodies were purified out of the solution by reverse affinity chromatograpy using an ÄKTA Start (GE Healthcare ÄKTA Start). The cleaved mixture was subjected to purification using a pre-equilibrated 1 mL HisTrap HP column (Cytiva) ...
-
bioRxiv - Biophysics 2020Quote: ... The proteins were then purified by cation exchange using a 5-mL HiTrap SP HP column (GE Healthcare) and the collected fractions analyzed by SDS-PAGE ...
-
bioRxiv - Biochemistry 2021Quote: ... The reducing agent was removed by passing twice through a NAP-5 desalting column (GE Healthcare, Chicago, IL) equilibrated with G-buffer lacking β-ME ...
-
bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
-
bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
-
bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and Sov was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
-
bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
-
bioRxiv - Biophysics 2020Quote: ... The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
-
bioRxiv - Biophysics 2022Quote: ... Supernatants were filtered by 0.45 μm and loaded onto a 5 mL HiTrap MabSelect SuRe column (GE Healthcare) equilibrated with COP Wash buffer (20 mM Hepes pH 7.4 and 500 mM NaCl) ...
-
bioRxiv - Biochemistry 2021Quote: ... 30 min) and the supernatant was loaded onto a 5 mL HisTrap HP Ni Sepharose column (GE Healthcare) equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
-
bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...