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Citations for GE Life Sciences :
1251 - 1300 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 100-250 mM NaCl and 2 mM DTT using the ÄKTA™ Pure 25 system (GE Healthcare).
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bioRxiv - Synthetic Biology 2021Quote: ... cell images were captured using the Cytell Cell Imaging System (GE Healthcare Life Sciences). To capture each fluorescent image ...
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bioRxiv - Bioengineering 2020Quote: ... using the IN Cell Analyzer 2500 and the IN Cell Developer toolbox (GE Healthcare). The average ATRX foci per cell was defined by the total ATRX foci / total nuclei from a minimum of 150 cells per experimental condition.
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bioRxiv - Genetics 2020Quote: ... The number of migrated cells was determined by In Cell Analyzer 2000 (GE healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The cell lines underwent selection in serum-free insect cell medium (HyClone, GE Healthcare) containing 7 μg/ml puromycin and 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... Both cell lines underwent selection in serum-free insect cell medium (HyClone, GE Healthcare) containing 7 μg/ml puromycin and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were imaged on an IN-Cell Analyzer 2200 (GE Healthcare, Location; 10×objective). Live/dead cell analysis was performed using IN Cell analysis software.
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bioRxiv - Genomics 2021Quote: ... Single Cell GenomiPhi (GE Healthcare), TruePrime (Expedeon) ...
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bioRxiv - Cell Biology 2019Quote: ... The detection of tubulin using mouse anti-tubulin KMX-1 primary (1:100; Gift from Keith Gull, Oxford) and anti-mouse-HRP secondary (1:20,000, GE Healthcare) was used to verify equal sample loading ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were affinity purified against DLI-1(1-368) coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone) at 3% v/v and Cell Boost 7b (Hyclone, GE Healthcare, Chicago, IL, USA) at 0.3% v/v on days 0 ...
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bioRxiv - Cell Biology 2020Quote: ... cells and purified from transfected cell supernatants using a HiTrap MabSelect SuRe column (GE Healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... The cell line has undergone selection in serum-free insect cell medium (HyClone, GE Healthcare) containing 7 μg/ml puromycin and 1% penicillin/streptomycin ...
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bioRxiv - Biophysics 2021Quote: ... Polyacrylamide gels were polymerized between two glass coverslips treated with 2% dimethyldichlorosilane (Plus One Repel Silane, GE Healthcare). For 0.6 kPa gels ...
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bioRxiv - Biochemistry 2020Quote: ... were subjected to the short column with 2 ml Sephacryl S-500 resin (GE Healthcare Life Sciences, IL) equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Microbiology 2020Quote: ... concentrated to 2 mg/ml by spin filtration and then run on a Superdex 200 column (GE Healthcare). MALS was conducted using a MiniDAWN and Optilab system (Wyatt Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs and the bound proteins were eluted with a gradient of 100-500 mM imidazole in 10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM DTE (IbpA) or applied to a HiLoad™ 16/600 Superdex™ 200pg column (GE Healthcare) in 20 mM Hepes pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: For mass spectrometry the beads were incubated with 150 µL the elution buffer (GE Healthcare, 2-1042-025) for 10 min at 4 °C while rotating ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Immunology 2022Quote: The binding kinetics of nanobodies to SARS-CoV-2 RBD were analyzed using SPR (Biacore 8K; GE Healthcare). The nanobodies were captured by the ProA sensor chip and serial dilutions of SARS-CoV-2 RBD were then flowed through at a rate of 30 μL/min in PBS buffer (with 0.05% Tween20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: ... proteins in 2% SDS were bound to Paramagnetic beads (SeraMag Speed beads, GE Healthcare, CAT#45152105050250, CAT#651521050502) on filter plates (Multiscreen ...
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bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
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bioRxiv - Genomics 2021Quote: ... 2 mM MgCl2) and then loaded onto two tandem 5 mL heparin HP pre-packed columns (GE Healthcare). Sen1 was eluted using a linear gradient from 20% to 100% of buffer B2 containing 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with ECL 2 western blot substrate and scanned with an ImageQuant LAS 4000 (GE Healthcare), and signals analyzed with ImageQuant TL 7.0 software (GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... a 2-elution step Ni2+-affinity chromatography was carried out using a Histrap HP 5 ml (GE Healthcare) on an FPLC apparatus (ÄKTA Pure ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (1×PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were developed using 1:1 Amersham ECL western blotting reagent (GE Healthcare) to SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Capto Core 700 beads (GE Healthcare; ~1:1 resin volume to sample volume) were used to remove residual dyes and enzymes from the solution of labeled virus ...
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bioRxiv - Biochemistry 2021Quote: ... a 1:1 mixture of 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) and 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-FBH1[1-250] in the pGEX6P-1 vector (Amersham/GE Healthcare/Cytiva) was introduced into BL21:DE3 Rosetta cells (Novagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with ~5.0×104 cells per well and taken images by IN Cell Analyzer 2200 (GE Healthcare) with 100× objective lens ...
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bioRxiv - Biochemistry 2020Quote: ... IN Cell Analyzer 6000 (GE Healthcare). Immunofluorescent intensities of H3K9Ac mark in the nuclei (total ~ 2500 – 4500 nuclei/treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Neuroscience 2019Quote: ... biotinylated anti-chicken (BA9010, 1:250, Vector Laboratories, CY3-streptavidin (1:500, GE Healthcare). Cell nuclei were counterstained with DAPI (0.2 mg/ml in PBS ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and incubated overnight with primary antibodies (aFLAG 1:2,500, aHSP90 1:50,000, GE Healthcare) containing 0.01% Sodium azide (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 then incubated with 1:50 (w/w) PreScission Protease (GE Healthcare) for 18 hrs at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The eluate was concentrated to 2 mL and then loaded onto a Superdex 200 column (GE Healthcare, Cat. #10034543) for size exclusion chromatography (S200 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM PMSF and 1x protease-inhibitor cocktail) and loaded onto a Superose™ 6 Increase column (GE Healthcare). Twenty-four fractions of 0.5 mL were collected and precipitated as described (Esteve-Bruna et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Two 2 ml aliquots were collected using GF/C glass microfiber filters (20 mm; Whatman, GE Healthcare Life Sciences), transferred to glass tubes ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Biochemistry 2022Quote: ... 2% glycerol and 250 mM imidazole and further purified on a Superdex 200 HiLoad 16/600 column (GE Healthcare) in the gel filtration buffer (30 mM HEPES pH 7.5 ...