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Citations for GE Life Sciences :
1151 - 1200 of 1389 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: Reconstituted vesicles were subjected to 4 freeze-thaw cycles and were then extruded with an Avanti Mini Extruder using a 0.4 µm-filter (GE Healthcare, Chicago, IL) 15 times ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown on the devices and the devices were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, IL, USA) which was placed on 1/2 MS medium supplemented with 0.5% sucrose and 1% agar ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated either using RBC lysis or using density centrifugation with Ficoll-Paque PLUS (GE Healthcare, IL). RBC lysis was performed with a 45-minute incubation with Dextran T500 (Pharmacosmos ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... after which it was passed through a 0.45-μm filter and loaded onto a pre-equilibrated 5-mL StrepTrap HP column (GE Healthcare, Chicago, IL) with bound SBP-FKBP12.6 fusion protein ...
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bioRxiv - Biophysics 2020Quote: The reconstituted liposomes were subjected to four freeze-thaw cycles followed by extrusion 15 times using an Avanti Mini Extruder fitted with a 0.4 μm-filter (GE Healthcare, Chicago, IL). For each assay ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA) as above.
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of each mucus sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), left to dry out ...
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bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were eluted and fractionated by size exclusion chromatography (SEC) using a Superdex Peptide 3.2/300 column (GE Healthcare, Chicago, IL, USA) at a flow rate of 10 μl min−1 using 30% (v/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... the pellet was resuspended in 18 mL of MCB1 with 0.1% BSA and 2-4.5 mL of material was layered onto stepwise Percoll (GE Healthcare, Chicago, IL, USA) MCB1 gradients with 0.1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Bands were detected using a chemiluminescent method with Chemi Lumi One Super (Nacalai Tesque) in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL).
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... and filtering through a 0.45-μm syringe filter followed by a 0.2-μm syringe filter (Whatman GE Healthcare Life Sciences, Chicago, IL). Sterile soluble mucin was then added to the fermentation buffer at a final concentration of 0.5%.
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bioRxiv - Physiology 2023Quote: ... Bands were detected using a chemiluminescent method with Chemi-Lumi One Super (Nacalai Tesque) in ImageQuant LAS 4000 (GE Healthcare, Chicago, IL). The primary antibody was a rabbit polyclonal anti-SLC26A4 antibody (PA5-115911 ...
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bioRxiv - Immunology 2023Quote: ... Each image captured 25% of the well and the percentage overlap was calculated using the Developer Toolbox software V1.9.2 (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare, Chicago, IL, USA) equilibrated with SEC buffer [20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2019Quote: Human PBMC were isolated by centrifugation of whole blood on Ficol-Paque (GE Healthcare) gradient for 30 minutes (500g ...
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bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
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bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
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bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...
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bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
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bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
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bioRxiv - Microbiology 2021Quote: ... The clarified supernatant was applied to a Ni2+-charged IMAC column with chromatography carried out on an AKTATM purification system (GE Healthcare, Chicago, IL). The loaded column was washed with additional IMAC binding buffer and then elution carried out using an elution buffer (0.5 M imidazole ...
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bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase-conjugated antibodies at room temperature for 1 hr before exposure on photographic film (28906844, Amersham, UK) following incubation with ECL for 1 minute (RPN2232, GE Healthcare, IL, USA) as per manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was adjusted to 35mM imidazole and loaded onto a 5ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) equilibrated in 20mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and purified using immobilized metal affinity chromatography via a HisTrapTM HP 5 mL (GE Healthcare, Chicago, IL, USA) and an AKTA FPLC system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleavage products were separated by gel electrophoresis on 15% denaturing polyacrylamide gel containing 7.5M urea and 24% formamide and visualized using a Typhoon 9400 Scanner (GE Healthcare Chicago, IL, USA). Activity assays performed with purified Argonaute proteins were carried out with 17 nt long guide G-2 (DNA ...
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bioRxiv - Biophysics 2022Quote: ... The eluate was concentrated and then applied to an initial size-exclusion chromatography (SEC) step using HiLoad 16/60 Superdex 200 (GE Healthcare, Chicago IL) in SEC200 buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
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bioRxiv - Physiology 2020Quote: Distinct bands of correct size were cut from 1.5% agarose gel and isolated using illustra GFX PCR and Gel Band Purification Kit (GE Healthcare, Chicago, IL, USA), following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA). The HisTrap HP column was washed with wash buffer (25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL GSTrap HP (GE Healthcare, Chicago, IL, USA). The GSTrap HP column was washed with wash buffer (25 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... containing 10 mM Tris pH 8.0 and 20 mM NaCl and purified using a SEC HiLoad 26/600 Superdex 75 column (GE Healthcare, Chicago, IL, USA) in 25 mM sodium phosphate pH 6.4 with 100 mM NaCl for wt-PD-1 and PD-1 mutants or in PBS pH 7.4 for PD-L1 and PD-L1-Long.
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated to 2 mL in a 30 kDa MWCO spin concentrator and applied onto a HiLoad 16/600 Superdex 200 pg size exclusion chromatography (SEC) column (GE healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The self-assembled crude octamers were purified by size-exclusion chromatography on a Superdex-200 10/300 GL column attached to an AKTA FPLC (GE Healthcare, Chicago, IL). Pure fractions were identified by 15% SDS-PAGE after coomassie staining ...
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bioRxiv - Biochemistry 2020Quote: ... a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.
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bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 5 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were analyzed by agarose gel electrophoresis with ethidium bromide staining and purified using the Illustra GFX PCR DNA and Gel Band Purification kit (GE Healthcare, Chicago, IL). Reactions (10 μL ...
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bioRxiv - Microbiology 2020Quote: ... through three in-line HEPA vent filters (0.3-µm glass-fiber to 0.2-µm PTFE to 0.1-µm PTFE; Whatman, GE Healthcare Life Sciences, Chicago, IL, USA) and stored at 4°C until use ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed using 0.1% PBSt and secondary antibody incubated 1h at room-temperature using Dako Polyconal Goat Anti-Rabbit or Anti-Mouse Immunoglobulins/HRP (Aglient Technologies, Santa Clara, CA, USA) washed using 0.1% PBSt and visualized using ECL or ECL Select (GE Healthcare, Chicago, IL, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2020Quote: ... Protein separation was carried out by SDS-PAGE in 10% bis-acrylamide gels and transferred to nitrocellulose membranes (Amersham Protran; pore size, 0.45 μm; GE Healthcare, Chicago, IL, USA). The membranes were blocked with 5% fat free milk in Tris-buffered saline (TBS ...