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Citations for GE Life Sciences :
1101 - 1150 of 1389 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... connected to a ÄKTA Pure apparatus equipped with a UV 280 nm flow cell (all from GE Healthcare, Chicago, IL, USA). EV fractions were collected based on UV absorbance ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were cleaned with an Illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Chicago, IL, USA) and sequenced by using Sanger technology in an ABI 3730XL sequencer (STAB-VIDA ...
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bioRxiv - Microbiology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were separated from blood samples by density gradient centrifugation using Percoll (GE Healthcare, Chicago, IL, USA). Whole blood was lysed with ACK lysing buffer to separate white blood cells ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... The recombinant proteins were purified from supernatants by affinity chromatography with a Ni-agarose column (HisTrapTM HP, GE Healthcare, Chicago, IL), dialyzed against PBS ...
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bioRxiv - Microbiology 2022Quote: ... Visualization of the corresponding bands was performed using the ECL™ Select western blotting detection reagent (GE Healthcare, Chicago, IL, USA) in a LAS-3000 detection system (Fujifilm España ...
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bioRxiv - Biophysics 2022Quote: ... and purified from any other oligomeric species and salts using a Superdex 75 10/300 GL column (GE Healthcare, Chicago, IL) at a flow rate of 0.5 ml/min in 20 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2019Quote: ... 60 - 120 µL of extruded sample were buffer-exchanged through 1.5- to 3.0-mL Sephadex G-50 Fine resin (GE Healthcare, Chicago, IL) columns equilibrated with buffer F (Table 2) ...
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bioRxiv - Microbiology 2021Quote: ... Both kinetic and equilibrium binding data were analyzed in the manufacturer’s Biacore 8K evaluation software version 1.1 (GE Healthcare, Chicago, IL). All experiments were performed at 37 °C in HBS-EP.
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bioRxiv - Immunology 2019Quote: ... CA) from continuous-flow centrifugation leukophoresis product using density centrifugation on a Ficoll-Paque gradient (GE Healthcare Life Sciences, Chicago, IL). Monocyte were isolated by adherence ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μL of each homogenate was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA) and left to dry out at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated Arr samples were injected into a Superdex 75 16/60 size-exclusion chromatography (SEC) column (GE Healthcare, Chicago, IL, USA) pre-equilibrated in SEC buffer for removal of aggregated protein ...
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bioRxiv - Cancer Biology 2021Quote: ... each lobe of lungs was separated and GFP signals were detected by Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL).
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bioRxiv - Cancer Biology 2021Quote: ... were used for the protein detection and membrane images were taken using Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL).
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bioRxiv - Microbiology 2021Quote: ... A glass column (150 cm x 2.4 cm) was packed with Sephadex LH-20 size exclusion gel (GE Healthcare, Chicago, IL) using a mobile phase of 10% methanol / H2O ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of the homogenate was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA) and left to dry out at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing Aβ40/Aβ42 were lyophilised and further purified using a Superdex 75 HR 26/60 column (GE Healthcare, Chicago, IL), and eluates were analyzed using SDS−polyacrylamide gel electrophoresis for the presence of the desired protein product ...
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bioRxiv - Immunology 2022Quote: ... Umbilical cord blood mononuclear cells (UCBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare, Chicago, IL). UCBMC were frozen in 10% DMSO/FetalPlex (GeminiBio ...
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bioRxiv - Physiology 2023Quote: ... Canada) under isoflurane anesthesia (Small Animal Imaging Facility at OSU) or a GE Vivid IQ Premium system (GE Healthcare, Chicago, IL) under ketamine/xylazine anesthesia ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was cleaned up using solid phase reversible immobilization (SPRI) beads (Sera Mag™, GE Healthcare Life Sciences, Chicago, IL, USA) in a homemade bead binding buffer containing 22% polyethylene glycol (PEG) ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... Canada) through 0.6 and subsequently 0.08 µm polycarbonate membranes (10417206 and 110604, Nuclepore Track-Etch Membrane; Whatman, GE Healthcare, Chicago, IL) sandwiched by polyester drain discs (230600 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were treated with EZ-ECL (Biological industries, Beit-Ha’emek, Israel) and visualized using ImageQuant LAS 4000 (GE Healthcare, IL, USA). Blot intensities were quantified using the FIJI ImageJ software86 ...
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bioRxiv - Neuroscience 2023Quote: ... The course of the radial nerve just above the elbow was visualized using ultrasound (LOGIQ e, GE Healthcare, Chicago, IL, USA). A high-impedance tungsten recording electrode was inserted percutaneously and with ultrasound guidance it was inserted into the nerve ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of each sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), and the dry membrane was incubated in NRP solution (0.01 g/ml KMnO4 in ddH2O ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by the appropriate horseradish peroxidase–conjugated anti-mouse (NA931V) or anti-rabbit (NA934V) secondary antibodies (GE healthcare, Chicago, IL, USA) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (AmershamTM Protran® 0.45 µm, GE Healthcare Chicago, IL, USA). Membranes were blocked for 1 hour with 5% BSA in TTBS (15 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Neuroscience 2024Quote: Quantitative analysis of the sagittal sections was performed using the automated microscope IN Cell Analyzer 6000 (GE Healthcare, Chicago, IL, USA). Four microscope slides were loaded into the system ...
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bioRxiv - Biophysics 2019Quote: ... The protein bands were subsequently imaged using an Amersham Imager 600 instrument and its accompanying software (v0.9.8) (GE Healthcare, Chicago, IL, USA) with a 1 s exposure time ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... The Nitrocellulose membrane was rinsed in phosphate buffered saline and placed in a phosphorimage cassette and a film (GE Healthcare, Chicago, IL) exposed for 24 hours ...
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bioRxiv - Microbiology 2019Quote: ... Affinity-purified proteins were polished by size-exclusion chromatography (SEC) on Superdex 75 or Superdex 200 columns (GE Healthcare, Chicago, IL, USA) equilibrated with buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... iPSC-moDC and MDM were washed with T cell expansion medium (RPMI supplemented with 10% HyClone FBS (GE Healthcare, Chicago, IL, USA), 1000 U/mL Penicillin/streptomycin ...
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bioRxiv - Biophysics 2021Quote: ... The column eluate containing cyt c was further purified through a gel filtration Superdex 75 16/600 column (GE Healthcare, Chicago, IL) phosphate buffer (25 mM sodium phosphate ...
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bioRxiv - Bioengineering 2020Quote: ... Small- (Superose 6 10/300 GL) or large-scale (Superose 6 pre-packed XK 26/70) SEC columns (GE Healthcare, Chicago, IL) were run with 500 µL or 15 mL of supernatant ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting sample was loaded onto a size exclusion column (HiPrep 16/60 Sephacryl S400 High Resolution, GE Healthcare, Chicago, IL, USA) equilibrated with filtered (0.2 μm ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were eluted from the column with a 10–200 mM imidazole gradient (100 mM Tris-HCl, pH 8.0, 200 mM NaCl) using an ÄKTA Prime Plus chromatography system (GE Healthcare, Chicago, IL). Fractions were collected and stored at 4°C and the presence of GSTU7-His protein was confirmed by analyzing the collected fractions by SDS-PAGE.
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bioRxiv - Plant Biology 2020Quote: ... two bead-based clean-up steps were carried out using SPRI beads (Magnetic SpeedBeads™, GE Healthcare No.:65152105050250, Chicago, IL, USA). The first PCR product was cleaned using a ratio of 1:0.9 and resuspended in 17 μL of ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... and immediately processed for isolation of peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation using Ficoll-Paque Premium 1.077 g/mL (GE Healthcare, IL; 17544202) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 10 μg of total protein was separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane with either 0.4 μm pores (GE Healthcare, Chicago, IL) or 0.2 μm pores (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were prepared from healthy individuals by centrifugation using Ficoll-Paque™ PLUS (GE Healthcare, Chicago, IL, USA). CD14+ cells were isolated by positive selection using anti-CD14 conjugated microbeads (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... The relative band intensities of the small RNA products were quantified by a Typhoon FLA 7000 image analyzer (GE Healthcare, Chicago, IL). The dsRNA-cleaving activity was calculated as the relative band intensity corresponding to the small RNAs in comparison with the total intensity of all bands in each lane ...
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bioRxiv - Molecular Biology 2020Quote: ... Flow rate was 0.75 ml/min and protein elution was monitored with the U9-L UV monitor (GE Healthcare, Chicago, IL, USA) at 280 nm.
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bioRxiv - Microbiology 2021Quote: ... Concentrated protein was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare, Chicago, IL, USA) column containing 0.003% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The FITC-TB_KKG6K bound to specific phosphoinositides was detected fluorometrically with a Typhoon FLA 9500 biomolecular imager (GE Healthcare, Chicago, IL, USA) equipped with a 473 nm laser and filters for excitation/emission wavelengths of 494/520 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... or normal rabbit serum (Abclon, Seoul, Republic of Korea) was cross-linked with Protein G Mag Sepharose beads (GE Healthcare, IL, USA), according to the manufacturer’s instructions ...