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Citations for GE Life Sciences :
951 - 1000 of 1353 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...
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bioRxiv - Cell Biology 2023Quote: ... The VacA-containing soluble fraction was loaded onto an anion exchange column (DEAE Sephacel, GE healthcare, Chicago, IL) pre-equilibrated with wash buffer ...
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bioRxiv - Neuroscience 2023Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Molecular Biology 2023Quote: ... Body composition was measured with dual-energy X-ray absorptiometry (DXA, LUNAR Prodigy, GE Healthcare, Chicago, IL, USA). Measures of total (LBM) ...
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bioRxiv - Biochemistry 2023Quote: Obtained gels and membranes were analyzed in Image Master 2D Platinum 7 Software (GE Healthcare, Chicago, IL, USA) to look for qualitative and/or quantitative differences in abundance/antigenicity of some venom proteins between the samples ...
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bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated with primary antibodies followed by peroxidase-conjugated secondary antibodies (GE HealthCare, Chicago, IL, USA). The antibodies utilized were rabbit monoclonal anti-pMEK2 (1:2000 ...
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bioRxiv - Immunology 2023Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Immunology 2024Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
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bioRxiv - Plant Biology 2022Quote: ... lysed and the protein purified by nickel affinity chromatography using the His- Trap HP column coupled with size-exclusion chromatography (SEC) using the HiPrep 26/60 Sephacryl S-200 column (GE Healthcare Life Sciences, UK). The protein was eluted into a buffer containing 30 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with a linear gradient of imidazole (0–1,000 mM) in His-tag-binding buffer using the ÄKTA FPLC™ Fast Protein Liquid Chromatography system (GE Healthcare, Princeton, NJ, USA). The eluted protein peak was collected and dialyzed against buffer D containing 50 mM Tris–HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluate from the His purification was then further purified by size-exclusion chromatography using a Superdex 200 HiLoad 16/600 column (GE Healthcare: 28-9893-35) in CSF-XB buffer ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified using MabSelect Protein A followed by Capto Q according to manufacturer protocols (GE Healthcare, Chicago, IL). Purified antibodies were concentrated and formulated to ≥25 mg/mL in PBS.
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were loaded onto a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare, Chicago, IL) equilibrated with 50 mM TRIS·HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... The mRNAs were further purified by filtration using MicroSpin™ S-200 HR columns (GE Healthcare, Chicago, IL, USA) to remove unreacted substrates (RNA reaction intermediates ...
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bioRxiv - Biochemistry 2021Quote: ... and radioactivity in bound and free RNA was quantified using Phosphorimager (GE) and ImageQuant 5.2 software (GE Healthcare, IL). Plots of the fraction bound RNA vs ...
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bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescent detection was completed with enhanced chemiluminescent (ECL) western blotting reagents (Catalog No. RPN2236, GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2020Quote: ... LUVs were subjected to size exclusion chromatography on a sephadex G25 PD-10 column (GE Life Sciences, Chicago, Il). The concentration of the encapsulated Indo-1 was estimated using fluorescence and known amounts of free Indo-1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Body fat percentage (BF%) was measured via dual-energy X-ray absorptiometry scans (LUNAR Prodigy; GE Healthcare, Chicago, IL) after overnight fasting.
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bioRxiv - Plant Biology 2022Quote: ... The resulting luciferase signal was detected with the digital camera system ‘ImageQuant LAS 4000’ (GE Healthcare, Chicago, IL, USA) and visualized with the open access software suite ‘Image J’ (National Institutes of Health ...
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bioRxiv - Immunology 2022Quote: ... The complex was purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were subjected to size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare, IL). The peak fractions were directly used for grid preparation with the reconstituted samples at ∼0.5 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Extracts (15⍰μg protein) were separated by SDS–PAGE and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL, USA). Membranes were blocked in TBS (150⍰mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Twenty-five µL of Protein A Sepharose beads (50% slurry contained phosphate-buffered saline buffer) (GE healthcare, Chicago, IL) was added to the resultant supernatant ...
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: MRI scans were performed on each participant using a GE Discovery MR750 3T MRI scanner (GE Healthcare, Chicago, IL). No participant was in the scanner for longer than 120 minutes per session ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was separated using SEC on a Superose 6 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with buffer containing 50 mM Tris-HCl (pH = 7.8) ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with horseradish peroxidase-conjugated sheep anti-mouse IgG polyclonal antibody (GE Healthcare, Chicago, IL, USA). Antigen-antibody interactions were detected using o-phenylenediamine solution as the substrate (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrated CM was loaded onto a column packed with Sepharose 4 Fast Flow resin (GE Healthcare, IL, USA). Eluates were measured at 280 nm absorbance to monitor the protein content of each fraction ...
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bioRxiv - Cancer Biology 2019Quote: ... detection was achieved with ECL™ or ECL™ prime western blotting detection reagents (GE Healthcare, Chicago, IL, USA). The antibodies used were ...
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bioRxiv - Bioengineering 2019Quote: ... A 7 mm hole punch was used to cut pieces of blood separation membrane (MF1, GE Healthcare, Chicago, IL) and amplification membrane (0.22 µm PES ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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Structural integrity following focused ultrasound thalamotomy and its correlation with tremor reliefbioRxiv - Neuroscience 2019Quote: All MR imaging studies were performed using the same 3T system (Discovery™ MR750, GE Healthcare, Chicago, IL, USA). Patients were recruited between 2016– 2018 and scanned prior to the procedure and in four follow-up scans ...
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bioRxiv - Bioengineering 2020Quote: ... and the protein:antibody complexes were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2019Quote: ... Whatman® Nuclepore policarbonate filters with pore size of 100 nm were purchased from GE Healthcare (Chicago, IL, USA). Black 96-well plates with transparent bottom (lumox® ...
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bioRxiv - Cell Biology 2021Quote: ... 300 mM KCl was performed using a PD-10 desalting column (GE Healthcare, Chicago, IL, Catalog #17-0851-01). Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... AAV particles was extracted in PBS and purified with an AKTA prime plus chromatography system (GE Healthcare, Chicago, IL) on an AVB Separose HP column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... sdRNA mimics and scrambled controls were ordered as custom miRIDIAN mimics from Dharmacon (GE Healthcare Dharmacon, Inc, Chicago, IL). Mimic and inhibitor sequences are detailed in Supplementary File 5 ...
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bioRxiv - Cell Biology 2021Quote: ... and the signal was captured by a CCD camera (ImageQuant LAS 4000 mini®, GE Healthcare, Chicago, IL, USA). As controls ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...