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Citations for GE Life Sciences :
1001 - 1050 of 3780 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The protein was further purified with Sephacryl S-300 HR (GE Healthcare) with PBS ...
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bioRxiv - Genomics 2022Quote: ... ribosome complexes were enriched using MicroSpin S-400 HR Columns (GE Healthcare) and RNA extracted using the Direct-zol RNA Miniprep Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2022Quote: ... were captured on a Streptavidin (SA) Series S Sensor Chip (GE Healthcare). Chip capture was performed in HBS-P+ buffer (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded onto a size exclusion chromatography column (S-100, GE Healthcare). Protein purity was checked by SDS-PAGE and the identity of the purified proteins was confirmed by MALDI-TOF mass spectrometry.
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bioRxiv - Biophysics 2023Quote: ... Anion-exchange chromatography with a HiTrap S column (GE Healthcare Life Sciences) was further used to remove potent DNA/RNA contamination ...
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was further purified through negative chromatography by passing through a GST-affinity column (GSTrap FF Columns, GE Healthcare Life Sciences) to remove free GST ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Biochemistry 2019Quote: ... 0.0027M KCl pH 8) and they were bound to a GST affinity column (GSTrap™ Fast Flow, GE Healthcare: 17-5131-02). The column was washed (0.02M NaPO4 ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α strain and purification of GST fusion proteins was achieved by incubation with Gluthatione Sepharose 4B matrix (GE Healthcare, Buckinghamshire, UK). Sumo proteins were kept bound to matrix ...
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bioRxiv - Cell Biology 2024Quote: ... The strips were then washed three times with 5 mL of TBST-BSA and incubated with anti-GST horseradish peroxidase–conjugated antibody (GE Healthcare, RRID:AB_771429) diluted in TBST-BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... and fluorescent images were acquired using the InCell Analyser 2200 system (GE Healthcare). Images then were processed in Fiji (Schindelin et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... and fluorescent size-exclusion chromatography (FSEC) (Superpose-6 10/300 GL, GE Healthcare) using buffer C (50mM Na-PIPES ...
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bioRxiv - Biophysics 2019Quote: ... Fluorescent micrographs were acquired using a DeltaVision widefield microscope (Applied Precision/GE Healthcare) equipped with UPlanSApo 60x (1.64 NA ...
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bioRxiv - Biophysics 2019Quote: ... Fluorescent micrographs were acquired using a DeltaVision widefield microscope (Applied Precision/GE Healthcare) equipped with UPlanSApo 60x (1.64 NA ...
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bioRxiv - Cancer Biology 2022Quote: ... Fluorescence imaging was carried out on DeltaVision high resolution fluorescent microscope (GE Healthcare) equipped with SoftWork software ...
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bioRxiv - Biophysics 2022Quote: ... Excess fluorescent dyes were removed by a PD-10 desalting column (GE Healthcare) with buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... Excess fluorescent dyes were removed by a PD-10 desalting column (GE Healthcare) in buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... followed by in-gel fluorescent scanning using the Typhoon FLA 9500 (GE Healthcare) and then the Coomassie brilliant blue staining ...
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bioRxiv - Biophysics 2020Quote: ... separated by SDS-PAGE and fluorescent imaged by Typhoon FLA 9500 (GE Healthcare). For the retrieved smFRET experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... and fluorescent images were taken using the InCell Analyser 2500 system (GE Healthcare). Chick embryos were fixed in 4% PFA for 2-3 hours at 40C and left in 30% sucrose solution overnight at 40C ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescent lipids were visualized on an image analysis system (STORM 860; GE Healthcare) and quantified with Quantity One software (Bio-Rad Laboratories).
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bioRxiv - Developmental Biology 2023Quote: ... and fluorescent images were acquired using the InCell Analyser 2200 system (GE Healthcare). Images then were processed in Fiji (Schindelin et al. ...
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bioRxiv - Cell Biology 2023Quote: Fluorescent microscopy was performed on an Olympus inverted microscope supported by Applied Precision and CellSense deconvolution systems ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified using a NiNTA purification system (HisTrap™ excel 1 mL) and size-exclusion chromatography using HiPrep™ 26/60 Sephacryl™ S-100 HR (GE Healthcare) in 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Biochemistry 2019Quote: Recombinant GST and GST-HIT proteins were produced by IPTG induction of transformed BL21 Rosetta strains and purified on 100 μl of Glutathione Sepharose 4B beads (17075601, GE Healthcare Life Sciences), for 4-5 hours at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... GST-fusions were purified from extracts of BL21 (DE3)/pLysS cells using Glutathione Sepharose 4B beads as recommended by the manufacturer (GE Healthcare Life Sciences). The kinase reaction was conducted at 30°C for 20 minutes and stopped by adding 2X Laemmli loading buffer ...
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2020Quote: ... of FZD7 containing the epitope for the F7-Ab (Glycine 168 to Serine 209) was cloned into the bacterial GST expression vector pGEX-4T3 (GE Healthcare Life Sciences). The epitope was shortened from either end through sequential cloning to produce 10 different length epitopes ranging from the original 42 aa to 8 aa ...
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...
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bioRxiv - Plant Biology 2019Quote: ... 6His–GST–CRK2cyto (WT, K353E and D450N) were expressed in Escherichia coli Lemo21 and purified by Glutathion Sepharose 4B (GE Healthcare, Chicago, USA). 6His–MBP–PLDα1 and 6His–MBP–CALS1_N were expressed in E ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was incubated for 4 hours at 4°C using a rotating wheel with 3 mL of a GST bead slurry (GE Healthcare Life Sciences) pre-washed 3x with wash buffer (WB ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... were amine coupled on to a Series S CM5 sensor chip (GE Healthcare) using 2 adjacent flow cells (i.e ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Cell Biology 2020Quote: ... bound to a cation-exchange column (Mono S 10/100 GL, GE Healthcare) with 50mM MES ...
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bioRxiv - Immunology 2020Quote: ... with either a research grade Series S Sensor Chip Protein G (GE healthcare) or Series S Sensor Chip CM5 (GE healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... All experiments were performed using an SA Series S Sensor Chip (GE Healthcare), which has four flow cells each containing streptavidin pre-immobilised to a carboxymethylated dextran matrix ...
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bioRxiv - Biochemistry 2020Quote: ... The Fab fragment was purified using ion exchange chromatography (Mono S, GE Healthcare; using a gradient of 10-500 mM NaCl in 20 mM sodium acetate ...
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bioRxiv - Biophysics 2020Quote: ... this dialysate was loaded onto a Resource-S cation exchange column (GE Healthcare) equilibrated in 50 mM NaCH3CO2 pH 5 and eluted with a 0-50% gradient of elution buffer (50 mM NaCH3CO2 pH 5 ...
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bioRxiv - Microbiology 2020Quote: ... the S glycoprotein was captured by StrepTactin Sepharose High Performance resin (GE Healthcare) and eluted with 10 mM D-desthiobiotin in the binding buffer (20) ...
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bioRxiv - Biochemistry 2021Quote: ... After Ub2 was purified by cation-exchange on a Mono S (GE Healthcare) column ...
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bioRxiv - Synthetic Biology 2019Quote: ... loaded with 750 μl of Sephacryl™ S-300 HR beads (GE Healthcare), which had an exclusion limit of 300 kDa ...
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bioRxiv - Biophysics 2022Quote: ... samples were loaded at 4 °C onto a Resource S column (GE Healthcare) equilibrated in 20 mM MES-NaOH pH 6.0 (for reactions containing FimCHisI or FimCHisIA) ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2,000 response units (RU) ...
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bioRxiv - Plant Biology 2022Quote: ... the culture filtrate was loaded onto HiTrap SP FF column(s) (GE Healthcare) equilibrated in buffer A (50 mM MES ...