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Citations for GE Life Sciences :
1251 - 1300 of 3780 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The measurements were done at flowrate of 0.5 ml/min over an S-75 Superdex 10/300 column (GE Healthcare) in 20 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... the protein precipitate was resuspended in 50 mM MOPS buffer (pH 7.0) and then applied to a Sephacryl S-100 column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Free dye was removed by size exclusion chromatography using a HiPrep 16/60 Sephacryl S 100 HR column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Biophysics 2020Quote: ... S-Trimers were purified to homogeneity by consecutive chromatographic steps including Protein A affinity column using MabSelect PrismA (GE Healthcare) which was preloaded with Endo180-Fc at (3 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque Inc.) supplemented with 10% v/v fetal bovine serum (FBS; Hyclone, GE Healthcare) and penicillin/streptomycin (Nacalai Tesque Inc. ...
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bioRxiv - Biophysics 2022Quote: ... The refolding mixture was dialyzed at 4 °C against 20 mM MOPS-NaOH pH 6.7 to remove residual GdmCl and applied to a Resource S column (GE Healthcare) to separate FimCHisI from free FimCHis ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Genomics 2023Quote: ... 50 ml of sea water was filtered through a 1.2 μm pore-size acrodisc (FP 30/1.2 CA-S cat N° 10462260 Whatman GE Healthcare Sciences) and used to inoculate culture flasks with 10 ml of filtrate each ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Optitran BA-S 85, pore size 0.45 µm, GE Healthcare) using a semi-dry blotting chamber at a constant current of 140 mA/gel for 60 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved SipAC was then passed through a HiPrep 26/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) using actin polymerization buffer [F-buffer ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The eluted fractions containing Cas9-NLS-His were diluted 4-fold with 50 mM Tris-HCl (pH 7.5) and subjected to cation exchange chromatography (Hitrap S HP, GE healthcare) pre-equilibrated with 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved protein was diluted further to 0.2M NaCl concentration and applied to cation exchange 10/10 Mono S column (GE Healthcare). Small terminase was eluted in two peaks with 0.2- 1M NaCl gradient ...
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bioRxiv - Biochemistry 2023Quote: ... The respective reactions were stopped by 20-fold dilution in 50 mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600 mM NaCl.
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was then purified on a HiPrep™ 16/60 Sephacryl S-500 HR gel filtration column (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Microbiology 2024Quote: 0.5 ml of 10.6 mg/ml BoGH13BSus or 8.2 mg/ml SusA was applied to a HiPrep 16/60 Sephacryl S-200 HR (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... The TLC plates were revealed by phosphorimaging (GE Healthcare) and analysed using ImageQuant software (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were covered with phosphor imaging plate (GE Healthcare) and signals was detected with Typhoon FLA7000 (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... The TLC plates were revealed by phosphorimaging (GE Healthcare) and analysed using ImageQuant software (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Plates were exposed to a phosphor screen (GE Healthcare) for 4 h ...
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bioRxiv - Cell Biology 2019Quote: ... as described previously for the non-fluorescent version (39): i) affinity purification of filtered supernatant with 5 ml HisTrap FF column (GE Healthcare) and step elution with 300 mM imidazole ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... The outcome of the activity assay was visualized with a fluorescent scan of an SDS-PAGE gel with Typhoon imager (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... The amount of Aβ42 bound to the cells was measured as mean fluorescent intensity using In Cell 6000 microscopy (GE healthcare, USA). The specific binding of Aβ42 was calculated by subtracting the fluorescence of cells in the absence of Aβ42 from that of fluorescence in the presence of varying concentrations of Aβ42 ...
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bioRxiv - Microbiology 2022Quote: ... Cells that integrated the fluorescent marker were selected on TSA with 15 µg/mL gentamycin and confirmed for their ability to expression the mCherry fluorescent protein using a Typhoon FLA 9000 imaging system (GE Healthcare). The gentamicin resistance cassette was flipped by electrotransformation of the pFLP3a plasmid containing the FLP-recombinase into the newly produced fluorescent cells.
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bioRxiv - Microbiology 2020Quote: ... Super-resolved fluorescent images were reconstructed with the corresponding recorded optical transfer function (OTF) in the SoftWoRx 7.0.0 software (GE Healthcare, Amersham, UK) at a Wiener filter setting of 0.006.
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bioRxiv - Microbiology 2022Quote: ... The free fluorescent probe was removed by a step of gel filtration chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) in the reaction buffer 50mM Tris (pH7.5) ...
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bioRxiv - Physiology 2019Quote: ... Analysis of fluorescent signals was performed using the Typhoon Trio laser scanner (λem=526nm, λex= 488 nm) and the ImageQuant software (GE Healthcare). TLC plates were stained with 470 mM CuSO4 in 8.5% o-phosphoric acid and subsequently incubated for 10 min at 180°C.
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bioRxiv - Biochemistry 2021Quote: ... ubiquitylation of fluorescein-labelled substrates was visualized with a fluorescent scan of an SDS-PAGE gel with a Typhoon imager (GE Healthcare) and quantified with ImageQuant (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... fully complementary to the fluorescent strand to avoid it re-hybridizing to the quencher strand) and 5 mM ATP (GE Healthcare) in a 100 µL reaction solution containing 10 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed 3x for 8min with TBST and the fluorescent signal from the membranes was recorded with an Amersham Typhoon (GE Healthcare). For each sample ...
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bioRxiv - Immunology 2019Quote: ... rVACV-eGFP-OVA-Early and rVACV-eGFP-OVA-Late were generated by transfecting plasmid DNA into BSC-1 cells infected with VACV-vRB21 at an MOI of 1 using the CellPhect Transfection Kit (GE Healthcare). The resulting rVACVs were plaque-purified 3× prior to characterization ...
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bioRxiv - Biophysics 2021Quote: ... the sample was concentrated to 2 mL and loaded onto a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Plin4 4T>S and csw 12mer were then further purified in a single step by cation exchange chromatography on a 20 ml Hiprep S HP column (GE Healthcare), as described for Plin4 AHs (Copic et al. ...
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bioRxiv - Molecular Biology 2020Quote: Purified Affimer proteins were incubated with KRAS lysates overnight at 4°C and the complexes purified by Ni-NTA affinity chromatography and size exclusion chromatography using HiPrep 16/60 Sephacryl S-100 column (GE Healthcare). The complexes were concentrated to 24 mg/ml (KRAS:K3 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples of TRIM21 CC 235 (100uL) were resolved on a Superdex S-200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min in 20 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the mixture was centrifuged for 2 mins and the supernatant was desalted by filtration with a Sephacryl S-400 HR column (GE Healthcare), centrifuged at 16 krpm ...
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bioRxiv - Genomics 2020Quote: ... Ribosomes were treated with 60 U TruSeq Ribo Profile Nuclease to generate ribosome-protected fragments and isolated via size-exclusion with an Illustra MicroSpin S-400 HR column (GE healthcare). Ribosomal RNA was depleted from both ribosome-protected fragments and RNA-seq libraries using the Illumina Ribo-Zero Gold Kit (Human/Mouse/Rat ...
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bioRxiv - Cell Biology 2020Quote: ... before heating at 95°C for 5 min and samples were separated by SDS-PAGE using a 4-16% acrylamide gradient gel and blotted onto nitrocellulose membranes (Hybond-C Extra, Amersham Bioscience or Optitran BA-S 85, GE Healthcare). The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako ...
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bioRxiv - Biochemistry 2019Quote: ... the sample was concentrated and then purified by size exclusion chromatography on a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... the purified sample was concentrated to 2 mL and loaded onto a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein containing fractions were concentrated to 3 mL and further purified by size exclusion using a HiPrep 16/60 Sephacryl S-500 HR (GE Healthcare) in Buffer D (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... The eluted protein was diluted to 50 mM NaCl with Buffer A before being loaded onto a cationic exchange Capto S column (GE Healthcare) equilibrated with Buffer A1cex (50 mM HEPES (pH 7.4) ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were treated with 5 units RNase I (ART-Seq) per OD260 and monosomes were purified in MicroSpin S-400 columns (GE Healthcare). For both RPF- and RNA-seq ...
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bioRxiv - Molecular Biology 2020Quote: ... for 45 min on a nutator (25oC) and monosomes were isolated from the void volume of Illustra MicroSpin S-400 columns (GE Healthcare). Small RNAs were extracted using the miRNeasy kit (Qiagen ...
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bioRxiv - Bioengineering 2020Quote: ... The ligase was inactivated with 2 M NaCl and the sample was injected into a Sephacryl S-1000 size exclusion column (GE Healthcare) to remove excess oligos and DNA ligase.