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Citations for GE Life Sciences :
951 - 1000 of 3780 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... plate 2 - 1.6 μg/μL carboxylate beads (1:1 mixture of hydrophilic and hydrophobic beads [mass/mass], GE Healthcare Cat ...
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bioRxiv - Cell Biology 2020Quote: Images were acquired on a Fluorescent microscope DeltaVision Core system (Applied Precision) with 100X Olympus UPlanSApo 100 oil-immersion objective (NA 1.4) ...
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bioRxiv - Cell Biology 2020Quote: ... Images were acquired on a Fluorescent microscope DeltaVision Core system (Applied Precision) with 100X Olympus UPlanSApo 100 oil-immersion objective (NA 1.4) ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... Fluorescent bands were detected on a Typhoon FLA 7000 (GE Life Sciences) imaging system ...
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bioRxiv - Cancer Biology 2022Quote: ... Images were acquired on the DeltaVision high resolution fluorescent microscope (GE Healthcare) equipped with SoftWork software ...
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bioRxiv - Biophysics 2020Quote: ... a cAMP to which the fluorescent dye Cy3B (GE Healthcare, Frankfurt, Germany) was linked via an aminohexylthio spacer to position 8 of the adenosine moiety (64 ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent signals on the gel were visualized with Typhoon 9400 (GE Healthcare).
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bioRxiv - Genomics 2024Quote: ... The fluorescent signals were detected using a DeltaVision deconvolution microscope (GE Healthcare). IF experiments were repeated at least three times ...
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bioRxiv - Cell Biology 2019Quote: ... One column contained total E.coli proteins covalently coupled to cyanogen bromide beads and the second column contained purified GST protein coupled to cyanogen bromide beads (GE Healthcare). Serum was cleaned by sequential incubations of 3 – 5 hours at room temperature with each column after which it was analyzed on an SDS page gel for lack of cross reactivity with GST and E.coli proteins from a total cell extract ...
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bioRxiv - Biochemistry 2019Quote: ... All constructs used for protein expression and purification in this study were GST fusions expressed from pGEX-4T1 (GE Life Sciences). The native proteins without signal peptides were expressed in BL21(DE3 ...
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bioRxiv - Biochemistry 2020Quote: ... Both the His-tagged and GST-tagged variants of PrgA were subsequently loaded on a Superdex- 200 16/600 size exclusion chromatography column (GE Healthcare) in 10 mM HEPES/NaOH (pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Following 3C protease treatment CDK2-cyclin E was buffer exchanged using SEC (Superdex 200 10/300) and then loaded onto a GST-trap column (5ml, GE Healthcare) and then onto an amylose resin (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... His- or GST-tagged proteins were captured from the lysate by the addition of 100 μL Ni Sepharose High Performance beads (GE Healthcare) or Glutathione Sepharose High Performance beads (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: Full-length Pak1 constructs (wild type and catalytically inactive) and full-length Sts5 constructs (wild type and phosphomutant) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare) for expression in BL21 DE3 Escherichia coli ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biochemistry 2019Quote: ... with expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved TPR-CHAT protein was eluted from GST beads and further fractionated by a Resource Q (AKTA Pure, GE Healthcare) in buffer ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant GST-RAPSYN was purified from the supernatant by GST-affinity chromatography (SA010010, Smart-Lifescience) and size-exclusion chromatography (17-0060-01, GE Healthcare). Purified GST-RAPSYN protein was digested with thrombin (T8021 ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... Cleaved GST-tag and bZip construct were separated by gel filtration chromatography using Hi-Load 16/600 Superdex 200 pg (GE Healthcare) on AKTA pure in a 20 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2024Quote: ... affinity columns of GST-fused Asct2(NT)-antigen and GST-fused Asct2(CT)-antigen were produced by conjugating the antigens with HiTrap NHS-activated HP (GE Healthcare). To purify anti-Asct2(NT ...
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bioRxiv - Immunology 2024Quote: ... An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... plates were imaged with a plate scanner (GE Healthcare Typhoon) in DsRed and GFP channels ...
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bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
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bioRxiv - Molecular Biology 2024Quote: ... Digested samples were passed through pre-washed (4x with 700 μl polysome buffer for 1 min at 450 x g) Sephacryl S-400 HR spin columns (GE healthcare, currently Cytiva), and centrifuged for 2 min at 450 x g at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were clarified by centrifugation (18,000×g for 60 min at 4°C) and incubated with glutathione Sepharose 4B (GE Healthcare, Uppsala, Sweden), applied to a gravity column ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant was transferred to a 50 mL tube containing 250 µl of Glutathione Sepharose 4B beads (GE Healthcare, 17075601) washed twice beforehand in 50 mL of PBS ...
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bioRxiv - Cell Biology 2021Quote: ... A volume of 2.5 ml of soluble fraction was incubated with 100 μl of Glutathione Sepharose beads (GE Healthcare, 17-0756-01) for 1 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed using an M-110S Microfluidizer (Microfluidics) and proteins were purified using glutathione chromatography according to the manufacturer’s instructions (GE Healthcare Life Sciences). To confirm the size and purity of purified recombinant protein ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was transferred to a new 50 ml falcon tube and 250 µl bed volume of PBS-washed glutathione Sepharose beads (GE Healthcare Australia) was added to the lysate ...
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bioRxiv - Cell Biology 2024Quote: ... the lysate was clarified by centrifugation and the supernatant was loaded onto either Talon® resin (Clonetech) or glutathione sepharose (GE healthcare) for initial purification ...
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bioRxiv - Cell Biology 2024Quote: ... 4°C for 30 min and added to a 50-mL Falcon tube holding 6mL of Glutathione-Sepharose® 4B resin (GE Healthcare) that was pre-equilibrated in the same buffer ...
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bioRxiv - Biophysics 2019Quote: ... to maximum of 10 mg/ml and further purified by size exclusion chromatography on a Superdex 200 HiLoad column (GE Healthcare; PfELC, MTIP, MTIP-S, MLC1, MLC1-S) or on a Superdex 75 HiLoad column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was gel filtrated on Sephacryl S-200 HR (GE Healthcare) equilibrated with NH100 buffer ...
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bioRxiv - Molecular Biology 2020Quote: Ni-NTA purified S proteins were further loaded into Superdex200 (GE Healthcare), the fractions were harvested and analyzed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM DTT) and loaded onto a Mono S column (GE Healthcare). Bound proteins were eluted by linear NaCl gradient with buffer CL and buffer CH (20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... at 25 °C using a Series S Sensor Chip NTA (GE Healthcare). CSP27 and CSP9 were immobilized on separate channels on the sensor chip surface as per the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... loaded onto a mono-Q or a mono-S column (GE Healthcare), equilibrated in buffer D (buffer C containing 100 mM NaCl) ...
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bioRxiv - Biochemistry 2020Quote: ... and by gel filtration on a Superdex S-20 column (GE Healthcare). Proteins were flash-frozen in 50mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... HCoV-OC43 S was purified using a StrepTrap HP column (GE healthcare). Purified proteins were concentrated ...
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bioRxiv - Systems Biology 2021Quote: ... footprint-containing monosomes were purified via MicroSpin S-400 columns (GE Healthcare) and footprints were purified with miRNeasy Mini kit (217004 Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... were captured on a Streptavidin (SA) Series S Sensor Chip (GE Healthcare). Chip capture was performed in HBS-P+ buffer (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein L sensor chip (GE Healthcare) at a density of 400 RU and experiments were performed in HBS-EP+ buffer at pH 6.0 following the method described above ...
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bioRxiv - Biochemistry 2020Quote: ... reactions were put over a MicroSpin S-400 HR column (GE Healthcare), which was equilibrated three times with 250 ml buffer containing 25 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Genetics 2019Quote: ... and loaded onto a Mono S PC 1.6/5 column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was gel filtrated on Sephacryl S-300 HR (GE Healthcare) equilibrated with NT350 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 150 s intervals using a MicroCal PEAQ-ITC instrument (GE Healthcare). Buffer was exchanged into 25 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2000 RU ...