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Citations for GE Life Sciences :
951 - 1000 of 1542 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Dot blots were developed using chemiluminescence (ECL BPS-Bioscience®) and visualized with a LAS Fujifilm 4000 system (GE Healthcare Life Sciences®).
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bioRxiv - Biophysics 2019Quote: ... and subsequently desalted and buffer exchanged (in to 20 mM phosphate buffer) using a HiPrep 26/10 Desalting column (GE Healthcare, Piscataway, NJ). The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Cancer Biology 2021Quote: ... Chemiluminescence detection was performed using the ECL Prime Western Blotting kit (GE-healthcare, RPN2232) and developed on a ImageQuant LAS 4000 (GE healthcare, 28-9558-10). Secondary antibodies for infrared fluorescence detection were donkey anti-rabbit-IRDye® 800CW (Rockland ...
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bioRxiv - Pathology 2019Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Plant Biology 2020Quote: ... A peroxidase-conjugated secondary antibody was used at a dilution of 1:200 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, USA; https://www.gelifesciences.com). Rubisco protein content was quantified from SDS-page gels using ALPHA-Ease FC software (Alpha Innotech ...
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bioRxiv - Cell Biology 2020Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL™ Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were HRP-conjugated and bands were visualized by the ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038–560) and bands were quantified with Gel Doc system (Bio Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to nitrocellulose membranes and visualized by enhanced chemiluminescence using ECL Prime Western Blotting Detection Reagent (#RPN2232, GE Healthcare; Chicago, IL, USA) and imager ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h incubated with the appropriate antibodies and then visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Microbiology 2020Quote: ... Rv1339 protein was visualized with a FujiFilm LAS-3000 Image Reader and the Amersham™ ECL™ Western Blotting Analysis system (GE Healthcare, UK).
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bioRxiv - Biochemistry 2023Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Physiology 2023Quote: ... Immunoreactive bands were detected using a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after the membranes were incubated with ECL Prime Western Blotting Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analysis of the immunoreactive bands was performed using the FluorChem FC3 Analysis Software.
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bioRxiv - Evolutionary Biology 2019Quote: ... ligation mix was diluted to 50µl and used 0.583:1 volume of home-brewed SPRI beads (SPRI binding buffer: 2.5M NaCL, 20mM PEG 8000, 10mM Tric-HCL, 1mM EDTA, oh=8, 1mL TE-washed SpeedMag beads GE Healthcare, 65152105050250 per 100mL buffer) for purification ...
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bioRxiv - Bioengineering 2019Quote: The TetR-family protein protein CAETHG_0459 was eluted in the same wash buffer containing a stepwise imidazole gradient (50-500 mM) following a buffer exchange performed using a HiTrap Desalting column (GE Healthcare #17-1408-01). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were developed using the WesternBright™ Quantum kit (K-12042-D10, Avansta) and visualized using an Amersham Imager 680 (GE Healthcare Life Sciences) system.
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bioRxiv - Genomics 2020Quote: ... Samples of equal volume from each fraction were loaded to a positively charged nylon membrane using a Slot Blot Filtration Manifold (PR648, GE Healthcare Life Sciences, PR648). The presence of BrdU on the nylon membrane was detected with an anti-BrdU antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoreactive bands were determined by exposing nitrocellulose blots to a chemiluminescence solution (Denville Scientific Inc., Metuchen, NJ) followed by exposure to Amersham Hyperfilm ECL (GE Healthcare Limited, Buckinghamshire, UK). To determine the relative amounts of protein densitometry Image J version 1.48v (National Institutes of Health ...
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bioRxiv - Synthetic Biology 2019Quote: ... A Bio-Rad ChemiDoc XRS imaging system was used for visualizing the protein blot after incubations with ECL plus reagent (GE Healthcare, catalog no. RPN2132).
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bioRxiv - Microbiology 2022Quote: ... The samples were transferred to a positively charged membrane using southern blot protocol from the Whatman Nytran SuPerCharge TurboBlotter kit (Whatman GE Healthcare, Little Chalfont, UK). Air dry the membrane ...
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bioRxiv - Microbiology 2022Quote: ... The denatured DNA was then blotted onto a Hybond LFP membrane using a Minifold I dot blot system (both from GE Healthcare, Piscataway, NJ, USA) followed by a wash with 400 µL TE buffer per well ...
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bioRxiv - Neuroscience 2021Quote: ... RNF5 injections were performed using HPS-EP+ buffer (GE Healthcare) as a running buffer at a flow rate of 30 µL/min for 180 s followed by a dissociation time of 1,200 s in 3-fold increasing concentration steps (0.5-44.3 nM) ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.0 buffer using a PD10 desalting column (GE Healthcare). The sample was collected into exchange buffer containing ten-fold molar excess of MTSL and stirred at room temperature overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Chip capture was performed in HBS-P+ buffer (GE Healthcare) to aim for ∼100-200 ligand response units (RU) ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with Lysis Buffer on an AktaPure FPLC (GE Healthcare). The column was washed with 15 column volumes (CV ...
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bioRxiv - Bioengineering 2021Quote: ... in binding buffer on an Äkta Pure FPLC (GE healthcare) with sample pump ...
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bioRxiv - Microbiology 2021Quote: ... and eluted with TN buffer using ÄKTA explorer (GE Healthcare). The ZomBC protein-containing fractions were collected and concentrated using an Amicon Ultra 10 K device ...
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bioRxiv - Bioengineering 2022Quote: ... Chip capture was performed in HBS-P+ buffer (GE Healthcare) to aim for ~100-200 ligand response units (RU) ...
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bioRxiv - Molecular Biology 2022Quote: ... buffer-exchanged by NAP-5 or PD-10 (GE Healthcare) into the storage buffer [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... After buffer exchange using a HiTrap Desalting column (GE Healthcare), the N-terminal polyhistidine-GST tag was cleaved by incubation with TEV protease at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200-1500 response units (RUs) ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200 -1500 response units (RUs) ...
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bioRxiv - Immunology 2023Quote: ... To the 20μL lysis buffer prewashed streptavidin beads (GE Healthcare) were added per sample and rotated for 2h ...
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bioRxiv - Genomics 2023Quote: ... and 25 ul Amersham™ Rapid-hyb Buffer (GE Healthcare) in a final volume of 50 μl using thermocycler (28 ...
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bioRxiv - Microbiology 2023Quote: ... equilibrated with buffer A2 using an AKTA system (GE Healthcare). Column was washed using buffer A2 and a linear gradient of glutathione (0-0.25M ...
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1 mL with buffer B and added immediately onto an anion exchange resin (Q resin; GE Healthcare; 0.2 mL; equilibrated in buffer H) and incubated (5 min) ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed as previously described using Horseradish Peroxidase (HRP)-conjugated secondary antibody followed by addition of ECL (GE Healthcare Bio-Sciences Corp., USA) to reveal chemiluminescence71 ...
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bioRxiv - Cancer Biology 2019Quote: ... washed three times for 15min using 1x TBS-T and developed using ECL Prime western blotting detection reagent (GE Healthcare Life Sciences, Pittsburgh, PA, USA). Each membrane was imaged using BioRad ChemiDoc and images analyzed using BioRad Image Lab software (BioRad) ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
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bioRxiv - Neuroscience 2021Quote: ... and subjected to single-step buffer exchange into SEC running buffer (see SAXS method) over a PD10 desalting column (GE Healthcare, Sephadex G-25 M matrix) under gravity flow ...
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bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged using a PD-10 column (GE Healthcare). Budding yeast Kip3 was purified following Kip3 purification (Gupta et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and buffer exchanged on a PD-10 desalting column (GE Healthcare) equilibrated in storage buffer (80 mM PIPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Pre-hybridization was carried out in Rapid-hyb Buffer (GE Healthcare) at 42° ...
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bioRxiv - Developmental Biology 2019Quote: ... The protein bands were developed by the ECL buffer (GE Healthcare, ECL Prime Western Blotting Detection Reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... equilibrated in buffer A connected to an FPLC-Prime (GE Healthcare). Following extensive washing with buffer A supplemented with 34 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... The protein was buffer exchanged using PD10 desalting column (GE Healthcare) prior to loading onto the anion exchange resin.
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bioRxiv - Cell Biology 2022Quote: ... FPLC-purified biotinylated proteins (ligands) in HBS-P+ Buffer (GE Healthcare) were captured on a Streptavidin (SA ...
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bioRxiv - Microbiology 2022Quote: ... with MOPS running buffer and transferred to cellulose membranes (GE Healthcare) with the Criterion Blotter system (Biorad) ...