Labshake search
Citations for GE Life Sciences :
801 - 850 of 1542 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... and detection using Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, Cat. #45-002-401). The chemiluminescence signals were captured with a ChemiDoc™ Touch Imaging System (Bio-Rad ...
-
bioRxiv - Genetics 2019Quote: ... we used the anti-rabbit HRP-conjugated antibody (1/5000 dilutions) with the ECL Western Blotting Detection System (GE Healthcare) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2021Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
-
Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and X-ray film ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
-
bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with either 1 ml of Amersham ECL substrate western blotting detection reagent (GE Healthcare lot# 9622301) or for higher sensitivity ...
-
bioRxiv - Bioengineering 2020Quote: ... and developed using the Amersham ECL Prime Western Blotting Detection Kit (catalog no. RPN2232; GE Healthcare Australia, Parramatta, NSW, Australia).
-
bioRxiv - Systems Biology 2022Quote: ... Immunoblots were carried out with the antibodies listed above and visualized using the ECL Western Blotting Detection Reagents (GE Healthcare). In order to strip antibodies from the blots ...
-
bioRxiv - Physiology 2022Quote: ... Proteins were visualized by chemiluminescence (#RPN2232 ECL and #RPN2235 ECL select Western blotting detection reagent – GE Healthcare, Little Chalfont, U.K.) and quantified using ImageLab software v ...
-
bioRxiv - Cell Biology 2020Quote: Secondary antibodies used for western blotting were: Amersham ECL Donkey anti-Rabbit IgG HRP (GE Healthcare NA934V, RRID:AB_772211, lot 16921443) and Rabbit anti-guinea pig IgG (H+L ...
-
bioRxiv - Immunology 2020Quote: ... and transferred by western blotting (30V, 1 hour) to polyvinylidene fluoride membrane (P 0.45 μm; 10600023 Amersham Hybond, GE Healthcare). Blots were probed with biotinylated GNA lectin (40 μg/ml ...
-
bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
-
bioRxiv - Immunology 2020Quote: ... the presence of β-actin was revealed (clone 8H10D10) Development was performed with ECL Western blotting reagents (GE Healthcare, UK).
-
bioRxiv - Neuroscience 2022Quote: ... were applied for 1 h at room temperature and detected with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences) in Fusion FX7 image and analytics system (Vilber Lourmat).
-
bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualised using Amersham ECL Western Blotting Detection Reagent and the ImageQuant LAS4000 luminometer (GE Healthcare Life Sciences). βIII-tubulin relative band intensities were quantified using ImageStudio software (LI-COR Biosciences ...
-
bioRxiv - Plant Biology 2023Quote: ... was used as secondary antibody together with the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare; Cat. No. RPN2232) for signal detection.
-
bioRxiv - Cell Biology 2023Quote: ... HRP activity was detected using ECL Prime Western Blotting Detection Reagent (Cytiva, Tokyo, Japan) and ImageQuant LAS 500 (GE Healthcare). Levels of β-catenin proteins were normalized to that of GAPDH using ImageJ software (National Institutes of Health ...
-
bioRxiv - Biophysics 2023Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using CL-Xposure films (Cat.no ...
-
bioRxiv - Biophysics 2024Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using the Chemidoc XRS imaging system (Bio-Rad)..
-
bioRxiv - Cell Biology 2024Quote: ... The detection was visualized with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences, Baie d’Urfe, QC, Canada). Quantification of band intensities (where indicated ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed three times and the signal was resolved with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and imaged on a ChemiDoc MP Imaging System (Bio-Rad) ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes incubated with HRP-conjugated secondary antibodies were incubated with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare #RPN2232) for 5 mins ...
-
bioRxiv - Molecular Biology 2024Quote: ... The membranes were washed five times and chemiluminescence was detected with ECL Prime Western Blotting Detection Reagents (Amersham, GE Healthcare). The signal was quantified with ImageLab software.
-
bioRxiv - Cell Biology 2020Quote: ... The blots and images were developed with the ImageQuant™ LAS500 Imaging System (GE Healthcare Bio-Science AB, Uppsala, Sweden).
-
bioRxiv - Molecular Biology 2020Quote: ... were used at 1:10,000 and blots developed with ChemiGlow detection reagent and visualized using ImageQuant LAS 4000 chemiluminescent camera (GE Healthcare).
-
bioRxiv - Cell Biology 2022Quote: ... These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime, GE healthcare). Chemiluminescence signals were detected by a LAS 4000 (GE healthcare ...
-
bioRxiv - Molecular Biology 2022Quote: ... were used at 1:10,000 and blots developed with ChemiGlow detection reagent (ProteinSimple) and visualized using ImageQuant LAS 4000 chemiluminescent camera (GE Healthcare). For RNA-Immunoprecipitations FLAG-Immunoprecipitation from DHX34-GFP-FLAG A5 cells were performed with in NET2 buffer (50 mM Tris-HCl pH7.5 ...
-
bioRxiv - Neuroscience 2022Quote: ... or nitration/phosphorylation were loaded to a final concentration of 36 ng into the Slot Blot system (GE Healthcare, Switzerland). The system was assembled with a pre-wetting nitrocellulose membrane and two Whatman filters in PBS for 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... mRNA isoforms were visualised and quantified using an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Bio-Sciences AB). The RiboRuler High Range RNA Ladder (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... a semi-dry transfer was done to blot the proteins in a 0.45 μm nitrocellulose membrane (GE Healthcare, Chicago, IL). Proteins were transferred for 90 minutes at 65 mA ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
-
bioRxiv - Immunology 2021Quote: ... buffer-exchanged with DPBS buffer (pH 7) and purified over Superdex 200 Increase 10/300 GL column (GE Healthcare) in DPBS buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare).
-
bioRxiv - Cell Biology 2023Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer, 0.150 M NaCl) using PD-10 desalting columns (GE Healthcare). Purity of the proteins were evaluated using SDS-PAGE electrophoresis stained with Coomassie blue.
-
bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
-
bioRxiv - Microbiology 2020Quote: ... Membranes were then rinsed three times and treated with the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences), and blots were analyzed with the ChemiDoc XRS+ System (Bio-Rad).
-
bioRxiv - Developmental Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE and examined by Western blotting using an Amersham imager 600 detection system (GE Healthcare). The following antibodies were used for immunoblotting ...
-
bioRxiv - Molecular Biology 2021Quote: ... HRP activity was detected using the Amersham ™ ECL ™ Prime Western Blotting Detection Reagent (GE Healthcare, Chalfont St Giles, UK) and a LAS4000 chemiluminescence imager (GE Healthcare Life Sciences ...
-
bioRxiv - Plant Biology 2021Quote: ... linked to horseradish peroxidase was diluted 1:10000 in TBS and used for one hour at RT before detection by chemiluminescence using AmershamTM ECLTM Prime Western Blotting Detection Reagent (GE Healthcare) following manufactures instructions.
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were separated on SDS-PAGE and transferred onto PVDF membrane and visualized using ECL Western Blotting Detection Kit (GE Healthcare). The primary antibodies used in this study were mouse anti-HA (1:2000 ...
-
bioRxiv - Microbiology 2020Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare). The signal was visualized in a Chemidoc MP imaging system (BIO-RAD).
-
bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
-
bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected using Western Lightning Plus-ECL (Perkin-Elmer, NEL104001EA) and images were acquired on an ImageQuant LAS4000 system (GE Healthcare). Densitometry analysis was carried out using ImageQuant TL software.
-
bioRxiv - Immunology 2020Quote: ... Ab binding (protein expression) was visualized using Amersham ECL Prime Western blotting detection reagent (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The following Abs were used in this study ...
-
bioRxiv - Biochemistry 2021Quote: ... Bands were visualised by enhanced chemiluminescence using ECL Prime Western Blotting detection reagents and using an ImageQuant LAS4000 system (GE Healthcare). ImageQuant TL 8.1 software (GE Healthcare ...
-
bioRxiv - Cancer Biology 2021Quote: ... Detection of proteins was performed with the enhanced chemical luminescence (ECL) method using the ECL Prime Western Blotting Reagent and image were acquired on an ImageQuant LAS 4000 Mini (GE Healthcare).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were detected by fluorescence (Odyssey, Li-Cor) or revealed using the ECL Prime Western Blotting Detection Reagent kit (GE Healthcare) and images were acquired in a dark room using a G:Box (Syngene ...
-
bioRxiv - Microbiology 2022Quote: ... Visualization of the corresponding bands was performed using the ECL™ Select western blotting detection reagent (GE Healthcare, Chicago, IL, USA) in a LAS-3000 detection system (Fujifilm España ...
-
bioRxiv - Biochemistry 2019Quote: ... protein bands were detected by exposing Amersham Hyperfilm to the membranes using an ECL solution Amersham ECL Western Blotting Detection Reagents (GE Healthcare).
-
bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were detected with HRP-conjugated goat anti-mouse or anti-rabbit IgG antibody and visualized with the ECL Western blotting substrate (GE Healthcare), according to the provided protocol ...