Labshake search
Citations for GE Life Sciences :
751 - 800 of 1542 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Blots were then probed with horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, GE Healthcare, NA934, RRID: AB_772206) for 1h at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Gel transfers were either performed at 300A for one hour onto a 0.45 μm (0.2μm for LC3 blots) nitrocellulose membrane (GE Healthcare) or transferred using the Trans turbo transfer system (Bio-Rad) ...
-
bioRxiv - Immunology 2024Quote: ... blots were incubated at room temperature for 5 min in the dark with ECL detection reagent (GE Healthcare), made up in accordance with manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... All blots were probed with an HRP-conjugated donkey anti-rabbit secondary antibody (catalog number NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse antibody (catalog number NXA931 ...
-
bioRxiv - Biochemistry 2021Quote: ... using buffer B (GE Healthcare Biosciences) and a flow rate of 1 mL/min ...
-
bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
-
bioRxiv - Physiology 2019Quote: ... diluted 1:5,000 and developed using Amersham ECL Western Blotting Detection Kit system (GE Healthcare Life Sciences, PA, USA).
-
bioRxiv - Cell Biology 2019Quote: For Western blotting protein samples were resolved by 10% SDS-PAGE and transferred to a PVDF membrane (GE Healthcare) by semi-dry blotting ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
-
bioRxiv - Cell Biology 2021Quote: ... After addition of the substrate (Femtoglow by Michigan Diagnostics or ECL Prime Western Blotting Detection Reagent by GE Healthcare), chemiluminescent signals were documented using A BioRad Chemi Doc imaging system ...
-
bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
-
bioRxiv - Microbiology 2020Quote: ... which were transferred to nitrocellulose membranes (Amersham Protran 0.45 NC Western blotting membrane, GE Healthcare Life Sciences, Munich, Germany) using a semi-dry Western blot procedure ...
-
bioRxiv - Cell Biology 2020Quote: ... Chemiluminescent detection was completed with enhanced chemiluminescent (ECL) western blotting reagents (Catalog No. RPN2236, GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection was performed using Amersham™ ECL Select™ Western Blotting Detection Reagent (Art. No. RPN2235, GE Healthcare) and a ChemiDoc™ XRS+ System (Art ...
-
bioRxiv - Cell Biology 2021Quote: ... and developed using BIO-RAD Clarity western ECL substrate (#170-5060) using ImageQuant™ LAS 4000 from GE healthcare Life Sciences.
-
bioRxiv - Microbiology 2021Quote: ... and the proteins were visualized by enhanced chemiluminescence (ECL) using the ECL Prime Western blotting detection reagent (GE Healthcare) and the ChemiDoc™ Touch Imaging System (Bio-Rad) ...
-
bioRxiv - Plant Biology 2021Quote: ... followed by washing as described above and detection with ECL Select Western Blotting Reagent (GE Healthcare, Little Chalfont, England) as described above.
-
bioRxiv - Biochemistry 2021Quote: ... The signal produced by reaction between HRP and ECL (Kit ECL Plus Western Blotting Detection System, GE Healthcare®) was detected by chemiluminescence using imaging Chemidoc (Biorad®).
-
bioRxiv - Cancer Biology 2019Quote: ... detection was achieved with ECL™ or ECL™ prime western blotting detection reagents (GE Healthcare, Chicago, IL, USA). The antibodies used were ...
-
bioRxiv - Cell Biology 2019Quote: ... Detection of the signal was performed using FluorChem Imaging System (ProteinSimple) with ECL Prime Western Blotting substrate (GE Healthcare) or SuperSignal WestPico PLUS Chemiluminescent Substrate (ThermoScientific).
-
bioRxiv - Bioengineering 2020Quote: ... and the protein:antibody complexes were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA).
-
bioRxiv - Neuroscience 2020Quote: ... Visualization of proteins was done by using Amersham ECL Western Blotting Detection Reagent and Amersham Hyperfilm (both GE Healthcare). Densitometric analysis of proteins was performed by ImageJ software ...
-
bioRxiv - Cell Biology 2021Quote: ... Wt CFTR and calnexin bands were exposed using Amersham ECL Western Blotting Detection Reagent (GE Healthcare Life Sciences, Canada) for 1-5 min exposures on the Li-Cor Odyssey Fc (LI-COR Biosciences ...
-
bioRxiv - Cell Biology 2019Quote: ... The membrane was washed by PBS-T and then developed by Amersham ECL Western Blotting Detection Reagent (GE Healthcare) and imaged with AI600 Chemiluminescent Imager system (GE Life Sciences) ...
-
bioRxiv - Immunology 2021Quote: ... The protein bands were detected using the enhanced chemiluminescence (ECL) Prime Western blotting detection system (GE HealthCare, Piscataway, NJ). The images of the blot were acquired on the ChemiDoc XRS using Bio-Rad ImageLab software (Bio-Rad laboratories ...
-
bioRxiv - Cell Biology 2020Quote: ... Signals from HRP-conjugated secondary antibodies were visualized by chemiluminescence (Amersham ECL start Western Blotting Detection Reagent, GE Healthcare) and recorded using the ImageQuant LAS 4000 Imaging System (GE Healthcare) ...
-
bioRxiv - Cell Biology 2021Quote: ... Membrane development was performed by an enhanced chemiluminescencebased detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare) and observed using a ChemiDoc MP Imaging System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2022Quote: ... in Can Get Signal Solution 2 (Toyobo) and visualized with ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232). Chemiluminescent signals were detected using a LAS-3000 Imaging System (GE Healthcare).
-
bioRxiv - Plant Biology 2023Quote: ... Detection was performed by ECL using the Prime Western Blotting Detection Reagent SuperSignal West Dura system (GE Healthcare, Amersham).
-
bioRxiv - Cell Biology 2024Quote: ... Visualization of the antibody binding sites was achieved using Amersham ECL Prime Western Blotting (GE Healthcare UK Ltd, RPN2232) according to the manufacturer’s protocol.
-
bioRxiv - Biochemistry 2020Quote: ... Blots were then washed 3 times in TBS-T and bands visualized with Amersham ECL chemiluminescence reagent (GE Healthcare). Blots were stripped (62.5 mM Tris-HCl pH 6.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... Blots were imaged using an AI600 Imager (Amersham) and quantified by ImageQuant TL (GE Healthcare, Pittsburgh, PA, United States).
-
bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
-
bioRxiv - Developmental Biology 2020Quote: ... blots were incubated with SuperSignal West Pico reagent (Pierce) then exposed to film (Amersham Hyperfilm MP, GE Life Sciences). Developed films were scanned and assembled in Adobe Illustrator CS5.1.
-
bioRxiv - Molecular Biology 2020Quote: ... The blot membrane was subjected to densitometric scanning and the band intensities were quantified using ImageQuant TL (GE Healthcare). The primary antibodies used in this study are as follows ...
-
bioRxiv - Developmental Biology 2021Quote: ... Blots were washed six times with TBST and developed with the ECL system (RPN2232, GE Healthcare Life Sciences, USA) according to the manufacturer’s protocols.
-
bioRxiv - Genetics 2019Quote: ... Blots were then washed an additional three times and detection was carried out using ECL detection regents (GE Healthcare). When required ...
-
bioRxiv - Microbiology 2022Quote: Southern blots were accomplished following the specifications of ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, UK). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: ... in the range of 20 pg to 200 ng were spotted on nitrocellulose filters (Ref Protran 0.45μm NC) using a slot blot filtration apparatus (GE Healthcare). The filters were blocked with skimmed milk and incubated with 306C7B3 IgG at 0.1 μg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
-
bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...
-
bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
-
bioRxiv - Biochemistry 2022Quote: RIP2K1-317 buffer exchange in buffer F was done by using a PD-10 desalting column (GE healthcare). 5mM MgCL2 and 2 mM ATP were added to the kinase sample ...
-
bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
-
bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
-
bioRxiv - Neuroscience 2020Quote: ... Blots were incubated with HRP-conjugated secondary anitbodies at 1:3,000 for 1 h at room temperature and bands developed using ECL Prime Western Blotting Detection Reagent (GE Healthcare). Blots were subsequently incubated with 1:20,000 HRP-conjugated β-actin (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare). Where representative blots are shown ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membranes were washed five times and chemiluminescence was detected with ECL Prime Western Blotting Detection Reagents (Amersham, GE Healthcare). The signal was quantified with ImageJ software.
-
bioRxiv - Molecular Biology 2021Quote: ... the development was performed using Pierce ECL Western Blotting Substrate (Thermo) and result analyzed with Amersham Imager 600 (GE Healthcare).