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Citations for GE Life Sciences :
951 - 999 of 999 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... were acquired every 3 min for 12 h with a 40× oil-immersion 1.3 NA objective using an Olympus DeltaVision Elite microscope (Applied Precision, LLC) equipped with a CoolSNAP HQ2 camera (Roper Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... All products were resolved on 12% polyacrylamide gels containing 8 M urea and visualized using Typhoon Trio fluorescence imager (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The eluate was cleaved with 3C protease overnight and Nes2G-SR11/12 was further purified by cation exchange chromatography on a HiTrap SP-FF column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Cancer Biology 2021Quote: Approximately 500,000 cells were transfected using a final concentration of 20 nM EPSTI1 (J-015094-09-0020 and J-015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 µL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Microbiology 2020Quote: ... Lysate equivalent of approximately 2×105 cells per well were run on NuPage 4-12% Bis-Tris Gel (Novex) and transferred to Amersham Hybond-P PVDF membranes (GE Healthcare). Membranes were blocked with 2% nonfat dry milk (Lab Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... Cat# 015094-12-0020) or non-targeting (D-001810-01-20 and D-001810-02-20) ON-TARGETplus siRNAs from GE Healthcare Dharmacon using 20 μL of Lipofectamine RNAiMAX (ThermoFisher #13778-150 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 – 300 µg protein was loaded on 10 – 12% SDS-PAGE and transferred at 4°C overnight to a nitrocellulose blotting membrane (A29434119, GE Healthcare) in transfer buffer (25 mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of lysates containing Laemmli buffer were heated at 95°C and electrophoresed on 12% polyacrylamide gels under standard SDS-PAGE conditions before being transferred onto a polyvinylidene fluoride membranes (GE Healthcare). Protein loaded membranes were blocked with 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved by SDS-12% polyacrylamide gel electrophoresis under standard conditions and transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham Hybond, GE Healthcare) using the Trans-Blot turbo transfer system (Biorad) ...
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bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... total protein extracts (10 to 20 μg) were subjected to 12% or 15% SDS-PAGE and then transferred to 0.45 μm nitrocellulose membranes (GE Healthcare, 10600003). The anti-CrATG3 polyclonal antibody was produced by injecting the recombinant wild-type CrATG3 protein into a rabbit using standard immunization protocols at the Animal Resource facility from the University of Sevilla ...
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bioRxiv - Cancer Biology 2023Quote: ... and separated by 4-12% Bis-Tris SDS-PAGE electrophoresis (at 180 V, 300 W, 55 min) and transferred onto a nitrocellulose membrane (GE Healthcare). Membranes were blocked in 5% BSA(Sigma ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved on 12–15% SDS–PAGE gels and then proteins were wet transferred to Protran nitrocellulose membranes (GE Healthcare) at 400 mA at 4 °C for 1.5 h in 1x Transfer buffer (25 mM Tris base ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved by electrophoresis on 10 or 12% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare). Total protein labeling was performed using No Stain Labeling Reagent (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts of protein extracts were electrophoresed on 12% sodium dodecyl sulphate (SDS) polyacrylamide gel prior to transfer on Hybond ECL nitrocellulose membranes (GE Healthcare). Homogenous transfer was confirmed by Ponceau Red staining ...
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bioRxiv - Cell Biology 2023Quote: ... Reduced immunoprecipitated proteins were separated on a 10 or 12% SDS-polyacrylamide gel and transferred to a PVDF membrane (GE Healthcare). Details on the blocking of the membranes and detection of the proteins can be found in Table S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Molecular Biology 2024Quote: ... or PIG-B (53,54) and fractionated on 10-12% polyacrylamide gel electrophoresis (PAGE)-urea gel and transferred to Amersham Hybond-N+ membrane (GE Healthcare). Transferred membranes were crosslinked 2x with a UV crosslinker (UVP ...
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bioRxiv - Microbiology 2020Quote: ... 10 μl of samples were loaded on 12% SDS-PAGE and transferred to Amersham Protran premium 0.2 μm nitrocellulose membranes (GE Healthcare Life Sciences) for 1 hour in an ice-cold transfer buffer (25mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Biophysics 2021Quote: ... The TLC plate was exposed to Fuji film BAS-MS 2025 imaging plate for 12 hr and analyzed using Typhoon FLA 9500 (GE Healthcare Ltd). The background obtained from reaction buffer (having no EccCb1 ...
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bioRxiv - Plant Biology 2022Quote: ... or adjusted to Chl/protein content were fractionated by electrophoresis on SDS-polyacrylamide gels (12%) and blotted onto nitrocellulose membranes (Amersham Protran, GE Healthcare, UK). Membranes were stained with Ponceau S and probed with protein-specific antibodies according to (Sambrook ...
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bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
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bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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bioRxiv - Neuroscience 2021Quote: The SH-SY5Y cells were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) and maintained in MEM/F-12 mixture containing 10% fetal bovine serum (FBS) (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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bioRxiv - Neuroscience 2020Quote: The human neuroblastoma SH-SY5Y cells purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) were maintained in MEM/F-12 mixture containing 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... 20 μg RNA samples were separated on a denaturing 12% polyacrylamide gel and transferred to a membrane (Amersham™ Hybond™ -N+ Membrane, GE Healthcare). Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10–12 % SDS-polyacrylamide gel electrophoresis gel (SDS-PAGE; Carl Roth) and transferred to nitrocellulose membranes (GE Healthcare Life Science). Membranes were blocked for 1 h with 5 % non-fat milk in PBS-T (PBS with 0.1 % Tween-20 ...
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bioRxiv - Pathology 2021Quote: ... Excess reagents were removed by repeated diafiltration into fresh PBS buffer (140 mM sodium chloride and 12 mM sodium phosphates at pH 7.4) using VivaSpin sample concentrators (GE Healthcare, 10,000 Da MWCO), and the volume was corrected to 200 μL ...
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bioRxiv - Microbiology 2021Quote: Whole cells lysate (50 µg) was separated on 12% SDS-PAGE and then transferred onto a PVDF membrane (GE Healthcare, Piscataway, NJ, USA). Membrane were blocked in 5% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μg total protein for each condition was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences) at 350 mA for 3 h for blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... For immunoblotting the platelet lysates were separated by a SDS-polyacrylamide gel electrophoresis (12 % SDS-polyacrylamide gel) and electro-transferred onto a nitrocellulose blotting membrane (GE Healthcare Life sciences). After blotting ...
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bioRxiv - Physiology 2024Quote: ... and a multi-frequency linear-array probe (12 L-RS, 5 – 13 MHz, 38.4-mm field of view, GE Healthcare, Milwaukee, WI, USA) were used to obtain ultrasonography images of the dominant VL and RF (based on kicking preference ...
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bioRxiv - Neuroscience 2024Quote: ... 25 μg of protein was separated on a 12% SDS-PAGE gel and transferred to a Hybond-LFP PVDF membrane (GE Healthcare, Illinois, USA). The membrane was blocked in 5% skimmed milk in 1X Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated in standard procedures using 12% polyacrylamide gel (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Life science, Germany) using TransBlot semi dry transfer cell (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: We employed a protocol similar to that previously described.12 The broad-spectrum kinase inhibitor Ki-NET (CTx-0294885) was conjugated to NHS-activated Sepharose4 Fastflow resin (GE Healthcare, Little Chalfont, UK). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
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bioRxiv - Biophysics 2022Quote: ... resolved in polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate (SDS-PAGE, 12% acrylamide) gels and transferred to PVDF membranes (GE Healthcare, Chicago, IL, USA) using standard procedures ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were denatured for 2 minutes at 95 °C and 12 µL were subjected to denaturing gradient gel electrophoresis (NuPAGE™ 12 %, Bis-Tris gel.) and subsequently transferred onto nitrocellulose membranes (GE Healthcare Life Sciences) for Western blotting against H3K36me3 ...
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bioRxiv - Physiology 2022Quote: ... and with an iodine-containing non-ionic radiocontrast agent iodixanol (12 mg I/kg bodyweight, Visipaque 320 mg I/ml; GE Healthcare, Chicago, Illinois, United States). The contrast agent was injected in the scruff area ...
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bioRxiv - Cancer Biology 2024Quote: ... Plates were dried at room temperature for at least 12 hours before images were captured using an Amersham Imager 600 (GE Healthcare UK Limited, Buckinghamshire, UK). Analysis was performed using ImageJ.