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Citations for GE Life Sciences :
851 - 900 of 999 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was passed through a filter paper (Grade 1; Size: 185 mm; Whatman, GE Healthcare Live Sciences), 1 mL of the flow through was transferred into a 1.5 mL centrifuge tube ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Imidazole (pH 8.0)) using a Hi-Prep Desalting 26/10 column (GE Healthcare). To remove the HIS-myc affinity tag ...
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bioRxiv - Molecular Biology 2024Quote: ... using a multi-frequency linear array transducer (3-12 MHz, GE Healthcare). Participants were instructed to lay on an athletic training table in the supine position 5 minutes prior to imaging ...
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bioRxiv - Biochemistry 2024Quote: ... following which 12 µl of a 50 % Glutathione Sepharose resin (GE Healthcare) was added to each sample ...
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bioRxiv - Plant Biology 2021Quote: ... A final purification step was performed by using a HiLoad 16/60 Superdex 200 preparative grade column (GE Healthcare) equilibrated in their respective Gel Filtration buffer (see below) ...
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bioRxiv - Microbiology 2020Quote: ... Ten μl of each culture were spotted onto 15 mm grade 1 Whatman filter discs (GE Healthcare Life Sciences). After drying for 1 minute at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the bound ligands were filtrated and collected on glass fiber papers (Whatman grade 934-AH, GE Healthcare, Pittsburgh, PA) with a M-24 Brandel filtration system (Brandel ...
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bioRxiv - Microbiology 2023Quote: ... 1 strip of 1 x 10 cm Whatman Grade 1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA) was added to a test tube containing 5mL of M63 media ...
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bioRxiv - Microbiology 2023Quote: ... and a 1×10 cm strip of Whatman Grade 1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA). As the filter paper was the only carbon source ...
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bioRxiv - Microbiology 2022Quote: ... and loaded to a Hi-Load 16/600 Superdex-200 pg (preparation grade) size-exclusion chromatography column (GE Healthcare) equilibrated with the gel filtration buffer (100 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Paris) by first amplifying plasmid mini preps with the Illustra TempliPhi Large Construct Kit (GE Healthcare) and subsequently labelling 2 μg of the amplification product by nick translation using Green UTP or Red UTP (Abbott Molecular ...
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bioRxiv - Biochemistry 2020Quote: ... The excessive imidazole was removed using a desalting column (Hi-Prep™ 26/10, GE Healthcare). The 8 × His-SUMO was cleaved by TEV proteinase and the cleaved fragment was removed through the affinity of the His-tag to a Ni-NTA column ...
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bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture VL muscle cross-sectional area (mCSA) ...
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bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture right and left leg images to determine VL muscle cross-sectional area (mCSA) ...
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bioRxiv - Developmental Biology 2021Quote: ... pH 4.0 were immobilized on a Biacore CM5 sensor chip (research grade) with the amine coupling kit following the manufacturer’s instructions (GE Healthcare). 1700 resonance units of FBN2-Ct or ADAMTS6-Ct was coupled to the chip for analysis in a Biacore 3000 instrument (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... the viral lysate was filtered through a 1.2 µm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 µm pore size Nalgene Rapid-Flow filter unit (PES ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluate was concentrated and run through a Superdex 200 prep 16/60 column (S200p; GE Healthcare) equilibrated in 20 mM HEPES (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... following which 12 μL of a 50% slurry of Glutathione Sepharose resin (GE Healthcare) was added ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture medium was MEM/F-12 mixture (HyClone; GE Healthcare Life Sciences) containing 10% fetal bovine serum (HyClone ...
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bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
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bioRxiv - Microbiology 2021Quote: ... HisTRAP eluted peak fractions were pooled and applied to a Hi-Load 16/600 Superdex-200 (preparation grade) size-exclusion column (GE Healthcare) equilibrated with the gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of protein sample diluted to a concentration of between 0.02 mg/ml and 0.05 mg/ml was added to a grid and then manually blotted away with Whatman Grade 1 filter Paper (GE Healthcare). 3 µl of 0.75% Uranyl Formate was then added to the grid and blotted away ...
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bioRxiv - Molecular Biology 2023Quote: ... As done in our previous experiments (Cattaneo et al., 2022) stimuli aliquots were spread on grade 1 – 20 mm circles filter paper (GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was loaded on a HiLoad™ 16/600 Superdex 200 preparation grade gel filtration column and installed in a liquid chromatography system (Aekta Purifier) from GE Healthcare. A protein size standard was used as a reference ...
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bioRxiv - Biochemistry 2024Quote: Gel filtration was performed using concentrated IMAC purified proteins of 500µl in volume using a Superdex 75 GL column and Superdex-200 10/300 GL column preparative grade size exclusion chromatography column (GE Healthcare). The column was pre-equilibrated with SEC buffer composed of 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
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bioRxiv - Cell Biology 2022Quote: ... The peak fractions were pooled and loaded onto a Superdex 200 HiLoad 16/60 preparative-grade column (GE Healthcare, 17-1069-01) in 5 mL injections and developed at room temperature with the size exclusion buffer (20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
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bioRxiv - Genomics 2021Quote: ... G-25 sephadex™ G-50 DNA grade resin columns were used to remove unincorporated γ-32P ATP according to manufacturer’s instructions (illustra™ GE Healthcare). The purified strand was used for subsequent annealing with equal amount of complementary strand in 50 μL total volume ...
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bioRxiv - Microbiology 2023Quote: ... equivalent to ∼1-3×108 cells per sample) were collected by vacuum filtration onto glass microfiber filters (grade GF/A, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... were collected by gentle vacuum filtration onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by gentle vacuum filtration of 1-2 L onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 2.0 mL centrifuge tubes (SafeLock ...
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bioRxiv - Microbiology 2024Quote: ... using HisTrap FF columns (Cytiva, MA) followed by size exclusion chromatography (SEC) on a HiLoad 16/600 Superdex 200 (preparation grade) column (GE Healthcare, IL). The purified trimers were flash-frozen in liquid nitrogen and stored at -80°C.
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bioRxiv - Molecular Biology 2022Quote: ... and a multi-frequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare, USA) was utilized to capture a VL image the transverse plane at approximately 50% the distance between the mid-inguinal crease and the proximal patella ...
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bioRxiv - Microbiology 2023Quote: Protein samples were run on 12% polyacrylamide gels and transferred to nitrocellulose membranes (GE Healthcare) via semi-dry transfer in a Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Biophysics 2023Quote: Proteins were resolved on 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes (GE Healthcare). The primary antibodies used were mouse anti-EHD2 (L-05 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated on 7.5-12% SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Blots were blocked 1 h with 10% dry milk ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins separated by 12% Tris-glycine SDS–PAGE were transferred to nitrocellulose membranes (GE Healthcare) and processed for immunoblotting using an ECL Prime kit (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... resolved on a 12% SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were incubated with 1:100 of the primary mouse antibodies ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown in 1:1 DMEM high glucose: Ham’s F-12 (Hyclone, GE Healthcare) supplemented with 5% fetal bovine serum (Hyclone) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown in 1:1 DMEM high glucose: Ham’s F-12 (Hyclone, GE Healthcare) supplemented with 5% fetal bovine serum (Hyclone) ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... Peptides were purified through a C18 prep column against an acetonitrile gradient on an AKTApure 25 FPLC (GE Healthcare) and confirmed by MALDI-TOF-MS (Bruker) ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed trice with 1× DPBS for 5 min each and then washed thrice for 20 min using HyPureTM molecular biology grade deionized distilled (DDI) water (GE Healthcare Life Sciences). Next ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were separated in 12% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare, #GE10600008) using standard procedures ...
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bioRxiv - Genetics 2021Quote: ... Protein lysates were resolved by 12% SDS-PAGE and transferred to PVDF membrane (GE Healthcare AmershamTM) using a Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: ... HyClone™ DMEM/F-12 (1:1; w/o phenol red) was purchased from GE Healthcare Life Sciences (Logan ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated using 12 %SDS-PAGE and blotted onto a polyvinylidene difluoride membrane (GE Healthcare). The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Extract samples were separated on 12% SDS-PAGE gels before transfer to nitrocellulose membranes (GE Healthcare). Membranes were blocked in PBS containing 5% milk before probing with primary antibodies at concentrations listed in Table S2 ...