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Citations for GE Life Sciences :
651 - 700 of 999 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The protein eluent was concentrated and further purified by size-exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with W1 buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was finally eluted by 230 mM imidazole and further purified through size exclusion chromatography (SEC, Superose 6, 10/300, GE Healthcare) with Lysis Buffer containing 0.001% LMNG ...
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bioRxiv - Microbiology 2021Quote: ... the clarified supernatant was purified on strep-tactin resin (IBA) followed by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare) equilibrated with 10 mM Tris-HCl pH 8.0 and 200 mM NaCl as the running buffer (SEC buffer) ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Cell Biology 2021Quote: ... Some proteins were further purified by size-exclusion chromatography on a Superose 6 10/300 or Superdex 200 Increase column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated in a vivaspin 100,000 MWCO and loaded onto a Superose 6 5/150 size exclusion chromatography column (GE Healthcare Lifescience) to remove thrombin and exchange the buffer to either 20 mm ...
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bioRxiv - Microbiology 2021Quote: ... the eluted proteins were concentrated to ∼200 µL and purified by size-exclusion chromatography on a Superose 6 10/300 GL column (GE Healthcare) in 10 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The cleaved protein was finally purified by size-exclusion chromatography (SEC) on a Superose 6 10/300 GL column (GE Healthcare) in 25 mM Tris (pH 8) ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 spikes were captured by StrepTactin beads (IBA) and further purified by gel-filtration chromatography using a Superose 6 column (GE Healthcare) with buffer containing 20mM Tris-HCl (pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... and the ones containing both His-SKA1/2+GFP-His-SKA2 subunits were pooled and further purified using size exclusion chromatography over a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated with gel filtration buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The separated species were concentrated to 1.3 - 2.5 mg/ml and 200 µl of each were applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer and connected to a miniDAWN TREOS II triple-angle light scattering detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P and ferritin) column (GE Healthcare) in 10 mM Tris pH=8 ...
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bioRxiv - Biochemistry 2022Quote: ... SEC was carried out on a Superose 6 Increase 10/300 GL column by fast protein liquid chromatography (ÄKTA; GE Healthcare); 0.25 mM fractions were collected ...
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bioRxiv - Microbiology 2021Quote: ... full-length gB-Fab complexes were evaluated by Native PAGE and purified on a Superose-6 column (GE Healthcare Life Sciences) into TBS pH7.4 to remove aggregates.
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and further purified with size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) in a buffer composed of 50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... and the elution was concentrated to 1mL and loaded onto the size exclusion chromatography using Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) pre-equilibrated with 50 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... followed by purification to remove unbound Fab via size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 column (GE Healthcare). Complexes were diluted to 30-50 µg/mL and immediately placed on 400-mesh Cu grids and stained with 2% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was concentrated using centrifugal concentrators (Amicon, 30 kDa molecular weight cut-off (MWCO)) and polished by size exclusion chromatography (SEC, Superose 6, GE Healthcare) in gel filtration buffer (30 mM HEPES/KOH pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... the NP and 1TD0-derived epitope probes were further purified by SEC on a Superose 6 10/300 GL column and a Superdex 75 10/300 GL column (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were further concentrated and purified by size-exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (GE Healthcare), pre-equilibrated with SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Active HAP fractions eluting from 0.2–0.3 M KPi were pooled and separated on a Superose 6 XK 16/70 gel filtration column (130 ml, GE Healthcare) equilibrated with buffer D + 0.1 mM EDTA at 0.15 M KCl ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was run over a 10/300 Superose 6 size exclusion column (GE Life Sciences, cat#17–5172–01). Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... concentrated using a 30 kDa molecular weight cut-off (MWCO) Ultra Centrifugal tube (Amicon) and loaded to a Superose 6-Increase 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: Analytical gel filtration was performed on a Superdex 200 Increase 10/300 GL column or Superose 6 Increase 10/300 GL (GE Healthcare) pre-equilibrated with 20 mM HEPES pH 7.5 ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... Cross-linked proteins were further purified from aggregation products by size exclusion chromatography on a Superose 6 increase column (GE Healthcare) equilibrated with size exclusion buffer (10 mM HEPES pH 7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
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bioRxiv - Immunology 2021Quote: ... The recombinant S-6P was purified by strep-tactin resin (IBA) following by size-exclusion chromatography on Superose 6 10/300 column (GE Healthcare) in 10 mM Tris pH 8.0 and 200 mM NaCl (SEC buffer) ...
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bioRxiv - Microbiology 2020Quote: Molecular mass determination was carried out using gel-filtration chromatography with a Superose 6 Increase 10/300 GL (GE Healthcare, USA) column ...
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bioRxiv - Cell Biology 2020Quote: Frozen aliquots of SARS-CoV-2 Spike trimer (Wuhan) and D614G mutant S trimer were thawed and purified by size exclusion chromatography (Superose 6 Increase 10/300, GE Healthcare) using 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The assembled capsids are subsequently purified from un-assembled dimer subunits using a Superose 6 10/300 GL size exclusion column (GE Healthcare). The small molecule allosteric modulators were purchased (Chem-Div ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... mosaic NP preps were stored for a month at 4°C and then analyzed via SEC using a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: P3 membranes were solubilized as described in the previous section and 200 μL of the detergent-solubilized supernatant was injected in a Superose 6 10/300 GL gel-filtration column (GE healthcare) equilibrated with 20 mM Tris-HCl pH 7.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved eluate was concentrated using a centrifugal filter (50 kDa cut-off, Amicon) and then loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare). The peak fraction containing recombinant SKI complex was collected ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was concentrated and purified by size exclusion chromatography (SEC) on a Superose 6 Increase 10/300 GL column (GE Healthcare) on ÄKTA™ Pure ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... at room temperature for 18 hours and subsequently the mAb-spike complexes were purified using SEC (Superose 6 Increase; GE Healthcare) with TBS as a running buffer and concentrated with 10 kDa cutoff Amicon ultrafiltration units ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted supercomplex solution was concentrated as described above and further purified by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare), preequilibrated with buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... using a Shimadzu HPLC LC-20AD/RF-20A (488 nmex, 512 nmem) instrument and Superose 6 10/300 column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: The GST-rNLRP1FIIND S969A-rDPP9 complex and rNLRP1FIIND proteins purified as described above were subjected to gel filtration (Superose 6, 10/30; GE Healthcare) in buffer containing 10 mM Tris pH 8.0 and 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Protein was purified from filtered cell culture supernatants by StrepTactin resin (IBA) and by size exclusion chromatography using Superose 6 column (GE Healthcare) in 10 mM Tris pH8,150 mM NaCl or 2 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was then mixed with equimolar KIFBP and subjected to size exclusion chromatography a second time on a Superose 6 column (GE Healthcare) equilibrated with gel filtration buffer ...
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bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...