Labshake search
Citations for GE Life Sciences :
751 - 800 of 1339 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Linker was purified by passing through a G-25 column (GE Healthcare, cat. # 27-5325-01) and stored at −20°C until use.
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bioRxiv - Cell Biology 2024Quote: ... SF650B-conjugated anti-rabbit IgG was then isolated using a Sephadex G-25 column (GE Healthcare). MRC-5 cells cultured on glass-based dishes for 2 days were subsequently incubated with 10 μg/ml anti-fibronectin IgG (F3648 ...
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bioRxiv - Immunology 2024Quote: ... then further purified using Gammabind Plus Sepharose Protein G 25 mL column (GE Healthcare, Life Sciences), with 20 min contact time ...
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bioRxiv - Developmental Biology 2024Quote: ... labeled antisense probes with RNA polymerases from Promega and RNA probes were purified with Illustra ProbeQuant G-50 microcolumns (GE Healthcare, cat. no. 28903408).
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bioRxiv - Microbiology 2024Quote: ... The cell lysates were incubated with FLAG-Trap Protein-G Sepharose (GE Healthcare, Milwaukee, WI, USA) solution for 4 h ...
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bioRxiv - Biochemistry 2024Quote: Anti-ASCT2 Ab was purified from the anti-sera using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... the reactions were purified by spin filtration using a Microspin Illustra G-25 column (GE Healthcare) and a Zeba spin desalting column (7K MWCO ...
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bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
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bioRxiv - Neuroscience 2020Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Neuroscience 2020Quote: Protein samples were separated by standard Laemli SDS-PAGE on 9% Tris-Glycine gels and transferred onto nitrocellulose membrane (GE Healthcare Bio-Sciences). PhosTag gels were prepared by adding 200 μM PhosTag™ (WAKO chemicals ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by standard ficoll density gradient centrifugation from 70mL heparinized blood immediately after blood withdrawal (GE Healthcare, Uppsala, Sweden). For the first cohort ...
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bioRxiv - Immunology 2024Quote: 1μg of purified fosmid/BAC DNA purified using standard alkaline lysis protocol was labelled with fluorescent dUTPs (SpectrumOrange and SpectrumGreen from Abott Molecular and Cy5-UTPs from GE HealthCare Life Science) in a 50μl nick-translation reaction for 3h at 15°C.
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples using standard Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated according to size using standard SDS- PAGE and transferred onto a nitrocellulose membrane (AmershamTM, GE Healthcare, Chalfont St Giles, UK). Membranes were blocked in 5 % skimmed milk/TBS-T and probed with primary antibodies (listed in supplementary table 1 ...
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bioRxiv - Biophysics 2021Quote: ... separated from free dye by gel filtration using a PD-10 column (GE Life Sciences, Chicago, IL), and diluted to a working lipid concentration of 144 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Genomics 2021Quote: ... Btbd7- and Zcchc8-knockout SIMS cells were maintained in phenol red-free DMEM (GE Healthcare/Cytiva, SH30284.01) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2021Quote: ... Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare, Marlborough, MA).
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bioRxiv - Microbiology 2020Quote: SPR spectroscopy and calibration free concentration (CFCA) assays were performed using a Biacore T200 device (GE Healthcare) and streptavidin-precoated Xantec SAD500-L carboxymethyl dextran sensor chips (XanTec Bioanalytics GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... The tag-free Fv fragment was concentrated and loaded onto a HiLoad16/60 Superdex75 column (GE Healthcare) equilibrated with TBS buffer ...
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bioRxiv - Microbiology 2022Quote: ... A488-Labelled phage was isolated from free dye by passing over two PD10 desalting columns (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: HESCs were grown in a six-well culture plate to 60%–70% confluence and transfected with 60 pmol of non-targeting siRNA (D-001810-10-05) or siRNAs targeting ACE2 (L-005755-00-0005) (GE Healthcare Dharmacon Inc., Lafayette, CO) in Lipofectamine 2000 reagent (Invitrogen Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... and 200ng Cas9 (PuroR) plasmid which express a puromycin resistant gene were co-transfected by 0.4 μL/well DharmaFECT Duo reagent (GE Healthcare Dharmacon, Cat #T-2010-03). After 48 hours of transfection ...
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bioRxiv - Microbiology 2023Quote: HeLa cells grown to 90% confluency were transfected with ON-TARGETplus human Skp1 SMARTpool siRNA or non-targeting siRNA (GE Healthcare Dharmacon, Inc., Lafayette, CO) at a final concentration appropriate to the vessel size ...
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bioRxiv - Molecular Biology 2020Quote: ... 200-300 µg of the indicated proteins were injected into a Superdex 200 10/300 GL (GE Healthcare) equilibrated at a flow rate of 0.3 ml per minute in 50 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples (20 µg) were separated on 15% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare). Membranes were blocked overnight at 4°C with 5% milk in TBS-T (20 mM Tris pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of proteins were resolved in Tris-acetate SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). For extracellular vesicle fractions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated in standard procedures using 12% polyacrylamide gel (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Life science, Germany) using TransBlot semi dry transfer cell (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: BM MNCs were isolated from each sample using a standard gradient separation approach with Ficoll-Paque PLUS (GE Healthcare Life Sciences, Pittsburgh, PA, USA). MNCs were enriched in CD34+ cells using magnetic separation with the CD34 Microbead Kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned with Sephadex G-50 fine medium (70g/L; GE Healthcare, Chicago, Illinois, USA), centrifuged through a 96-well filter plate (Phenix Research ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-incubated with 50 μl Protein A and Protein G Sepharose (GE Healthcare 17513801 and 17061801) mix for 3~4 h and then incubated with WCE overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected from selected hybridoma clones and purified through a protein G-sepharose column (GE Healthcare). mAbs were isotyped with Pierce rapid isotyping kit (Cat#26178) ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the purified protein were pooled and desalted with PD midi Trap G-25 (GE Healthcare) using the spin protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Unincorporated nucleotides were removed by passing the sample over two Illustra MicroSpin G-50 columns (GE Healthcare) and EDTA was added to a final concentration of 5 mM ...
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bioRxiv - Neuroscience 2020Quote: ... Immune complexes were isolated by the addition of 20 μL of protein G– Sepharose beads (GE Healthcare) followed by incubation for 2 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Microbiology 2021Quote: ... and purified from unincorporated nucleotides by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare, USA). Hybridisation was performed overnight at 50 °C in ExpressHyb Hybridisation Solution (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Microbiology 2021Quote: ... Unincorporated nucleotides were then removed by gel filtration on Illustra MicroSpin G-25 Columns (GE Healthcare, USA) and hybridisation was performed overnight at 40 °C in ExpressHyb Hybridisation Solution (Clontech ...
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bioRxiv - Immunology 2022Quote: ... followed by Ni-NTA purification and desalting by PD MiniTrap G-25 columns (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were either stained with Coomassie brilliant blue G-250 or transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in 0.05% Tween TBS at room temperature for 1 h and then incubated at 4 °C overnight with a specific polyclonal serum produced by immunization of mice with RBD produced in HEK-293T cells ...
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bioRxiv - Microbiology 2020Quote: ... The PhuS or PhuS H212R mutant lysate was applied to a Sepharose-G column (GE Life Sciences) equilibrated with 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).