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Citations for GE Life Sciences :
9051 - 9100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies used in Western blotting and IF were HRP sheep anti-mouse IgG (GE Life Sciences NA931V), goat anti-mouse IgG IRDye800CW (LiCoR 926-32210) ...
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bioRxiv - Biophysics 2022Quote: ... the sample was passed through Superdex peptide 10/300GL column (GE healthcare); connected to Biologic Duoflow model Bio-Rad fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2022Quote: ... Eluted mAb92 was further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in phosphate buffered saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... The mAb was purified using five milliliter HiTrap Protein G HP columns (GE Healthcare) equilibrated in 20 mM sodium phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... followed by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was flowed over one milliliter HiTrap Protein G HP columns (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl using an AKTA Flux system (GE Healthcare). The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2022Quote: ... The complex was then purified and excess Fab92 removed by SEC using a Superose 6 Increase 10/300 column (GE Healthcare), equilibrated with 10 mM Tris pH 8 ...
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Immunology 2022Quote: ... The collected single cell suspension was enriched with 40% Percoll (GE Healthcare) plus centrifugation at 400 g for 20 min ...
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bioRxiv - Immunology 2022Quote: ... or a DeltaVision OMX SR Imaging System (GE Healthcare). Reconstruction of super-resolution images was done with softWoRx (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... Reconstruction of super-resolution images was done with softWoRx (GE Healthcare). Live-cell microscopy images were acquired using an environmental chamber (37°C ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2022Quote: ... Membranes were imaged using an Amersham Imager 600 (GE Life Sciences), and densitometry was performed using ImageJ software ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated from human CMV-seropositive buffy coats by density centrifugation over Ficoll Paque Plus (GE Healthcare) and screened for the presence of NKG2C+ NK cell expansions by flow cytometry 51 ...
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bioRxiv - Microbiology 2022Quote: ... Extracted DNA was quantified using a Nanovue Plus spectrophotometer (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2022Quote: ... and quantified using a Nanovue Plus spectrophotometer (GE Healthcare Life Sciences). cDNA was generated by retrotranscription of 1 g of total RNA using M-MLV reverse transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were loaded into a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated with binding buffer (50 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... Ten µg of a 1:1 mixture of hydrophilic and hydrophobic carboxyl-coated paramagnetic beads (SeraMag, #24152105050250 and #44152105050250, GE Healthcare) were added for each µg of protein ...
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bioRxiv - Immunology 2022Quote: The reagents used in this work were as indicated: CypHer5E (GE Life Sciences, PA15401); CellTrace Yellow (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... the target dsDNA from the PCR amplification was first concentrated by ethanol precipitation and then purified on an Akta Pure 150 FPLC instrument (GE Healthcare, USA) with a GE HiPrep 26/60 Sephacryl S-500 High Resolution size exclusion column and 0.3M NaCl running buffer ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... hematopoietic cells were enriched by density gradient centrifugation with 40/70 Percoll (GE Healthcare) for 20 min at 700xg.
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bioRxiv - Microbiology 2022Quote: ... size exclusion chromatography was performed using a Superdex 200 increase 10/300 column (GE Healthcare). The protein concentration was determined by the Bradford method using bovine serum albumin as the standard ...
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bioRxiv - Immunology 2022Quote: ... lymphocytes were isolated by a discontinuous 40/80% Percoll gradient (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... liver and enzyme treated lung were prepared by meshing organs through 70 μm nylon cell strainers in sRPMI-1640 with 8% FBS (HyClone Laboratories, GE Healthcare, Logan, Utah). After red blood cells lysis with NH4Cl (liver ...
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bioRxiv - Immunology 2022Quote: ... The resulting pellet was resuspended in 6 ml of 40% Percoll (GE Healthcare) and layered on top of 70% Percoll to isolate leukocytes ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% fetal bovine serum (FBS; HyClone, GE Healthcare, Chicago, IL), 40 μg/ml gentamicin and 0.01M Tricin (both from Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... The concentrated samples were loaded on a Superose 6 10/300GL column (GE Healthcare) and an isocratic run (1.2 column volume ...
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bioRxiv - Microbiology 2022Quote: Samples were run on a 10% SDS-PAGE gel and then either stained with Coomassie Brilliant Blue or transferred to a 0.45 µm-pore-size nitrocellulose membrane (GE Healthcare) for immunoblotting with mouse monoclonal anti-S-peptide antibody (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... were collected by gentle vacuum filtration onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by gentle vacuum filtration of 1-2 L onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 2.0 mL centrifuge tubes (SafeLock ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a last step in which the protein was dialyzed against buffer LS and then applied onto a HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Microbiology 2022Quote: The supernatant was then applied onto a 5 ml HisTrap HP column (GE Healthcare, USA) equilibrated with buffer BZ3 ...
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bioRxiv - Microbiology 2022Quote: ... flow-through fractions from the second Ni-NTA affinity purification step containing the protein at high purity were concentrated and then applied onto a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare, USA) equilibrated with a buffer containing 50 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... and the eluted fraction was desalted on a Sephadex G-25 column (PD-10, GE Healthcare) equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... adjusted to pH 8 with HCl) and applied to an HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... and exposed on X-ray films (Kodak Biomax MR-1) or visualized using GE ImageQuant LAS4000 camera (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2022Quote: ... Primary antibody labelling was done for 1 hour using mouse anti-BrdU (GE Healthcare) and rat anti-HA (Roche ...
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bioRxiv - Microbiology 2022Quote: ... and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were then blocked with 5% skim milk in 50 mM Tris-HCl [pH 7.6] ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluant was concentrated and further purified using a Superdex-200 (GE Healthcare) column equilibrated with a buffer containing 10 mM Tris–HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... and the Csx30–Csx31–RpoE complex was purified by Ni-NTA Superflow resin and a HiLoad 16/600 Superdex 200 column (GE Healthcare). The target RNA (25 nucleotides plus 5′ GG ...
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bioRxiv - Microbiology 2022Quote: ... MV-containing fractions of size exclusion chromatography (SEC; sepharose CL-2B, ref.:GE healthcare, Little Chalfont, UK) were pooled and concentrations were measured by tunable resistive pulse sensing using qNano Gold (Izon Science Ltd. ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Microbiology 2022Quote: ... Both the 6His- and Strep-tagged proteins were then further purified by SEC on an ÄKTA Purifier using a Superdex 200 16/600 GL column (GE Healthcare), and simultaneously buffer-exchanged into 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... they were transferred onto Amersham Hybond ECL nitrocellulose membrane (GE Healthcare). The wet transfer was carried out for 1 h at 100 V in ice-cold buffer (39 mM glycine ...
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bioRxiv - Microbiology 2022Quote: SEC-MALS was performed using an ÄKTA Prime system with a S200 10/300 GL column (GE Healthcare) and a MALS detector (Wyatt) ...