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Citations for GE Life Sciences :
8901 - 8950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Microbiology 2022Quote: ... IgG was isolated using 5 ml HiTrap Protein G High Performance column (GE Healthcare), whereas IgM was isolated using POROS™ CaptureSelect™ IgM Affinity Matrix (ThermoScientific ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% (v/v) fetal calf serum (Hyclone, GE Healthcare), 1% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then loaded onto a Hybond-N+ membrane (GE Healthcare), allowed to air dry and crosslinked twice at 200000 μJ/cm2 UV ...
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bioRxiv - Molecular Biology 2022Quote: ... were purchased from GE Healthcare Dharmacon ...
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bioRxiv - Molecular Biology 2022Quote: ... separated by electrophoresis and transferred to a nylon membrane Hybond N+ (GE Healthcare). The probes for the upstream regions of the pks1 (oligonucleotides 9 and 10 ...
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bioRxiv - Genetics 2022Quote: ... 2 ul of Streptavidin Sepharose High Performance beads (GE Healthcare) and 35 ul of H2O ...
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bioRxiv - Genetics 2022Quote: ... or goat-developed anti-mouse immunoglobulin G (GE Healthcare, NA931). Membranes were developed with ECL+ and signals were obtained using ChemiDoc XRS+ and analyzed by Image Lab software (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... and the antibodies were purified by protein A affinity chromatography (GE Healthcare Life Sciences) as previously described31 ...
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bioRxiv - Immunology 2022Quote: ... respectively (both GE Healthcare, Chicago, IL), eluted with 100 mM glycine pH 2.2 ...
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bioRxiv - Immunology 2022Quote: ... the complex was purified by size exclusion chromatography (Superdex 200 Increase size exclusion column, GE Healthcare, Chicago, IL) in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... and cells were resuspended in PBS-EDTA (room temperature) and layered onto Ficoll Paque (1.077 g/mL; GE Healthcare Europe GmbH). After centrifugation (800 x g ...
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bioRxiv - Immunology 2022Quote: The peripheral blood mononuclear cells (PBMC) were extracted using Ficoll-Paque (GE Healthcare, Chicago, IL, USA) gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: ... and EPICERTIN were also determined by surface plasmon resonance (SPR) using the Biacore Gold Seal T200 (GE Healthcare) equipped with a CM5 sensor chip as previously described 19 ...
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bioRxiv - Immunology 2022Quote: ... bone marrow from the femurs and tibias was harvested and layered over Ficoll-Paque PLUS (GE Healthcare). Cells were harvested from the interface ...
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bioRxiv - Immunology 2022Quote: ... the target dsDNA from the PCR amplification was first concentrated by ethanol precipitation and then purified on an Akta Pure 150 FPLC instrument (GE Healthcare, Chicago, IL, USA) with a GE HiPrep 26/60 Sephacryl S-500 High Resolution size exclusion column and 0.3M NaCl running buffer ...
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bioRxiv - Biophysics 2022Quote: ... It was extruded through a 0.1 μm filter (Whatman Anotop, GE Healthcare, USA) to create unilamellar vesicles and stored at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Anion exchange chromatography was performed on an ÄKTA Pure system equipped with a 10/100 Tricorn column (Cytiva) packed with Source 15Q ion exchange resin (Amersham Biosciences, GE Healthcare) equilibrated in AEX buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and washed with 10 column volumes washing buffer (20 mM Tris, 500 mM NaCl, pH 7.8) using an AKTA explorer 100 (GE Healthcare). ARF-DBD proteins were eluted by 1 h incubation with 40 mM DTT in washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... with 10% fetal bovine serum (Hyclone, GE Healthcare, Boston, MA, USA), 100 U/ml penicillin ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant of cell lysate was purified over Glutathione-Sepharose 4B (GS4B) (GE Healthcare, Boston, MA, USA) or Ni-NTA resins (Qiagen) ...
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bioRxiv - Biochemistry 2022Quote: ... and gel filtration chromatography (Superdex 200, GE Healthcare). GST-tagged SYT16 peptide (132KLPHVLSSIAEEEHH147L) ...
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bioRxiv - Biochemistry 2022Quote: ... thrombin or PreScission protease and proteins were further purified by ion exchange chromatography (Source 15Q, GE Healthcare) and gel filtration chromatography (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... the labeled KRAS protein was purified by size-exclusion chromatography using 16/60 Superdex75 increase column (GE Healthcare) pre-equilibrated with 20 mM HEPES (pH 7.0) ...
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bioRxiv - Biochemistry 2022Quote: ... The diluted sample was loaded onto tandem HiTrap Q (5ml, GE Healthcare) and HiTrap Heparin (5 ml ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2022Quote: ... 10mM βME) and loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was passed over a 5 ml Ni-NTA Fast Flow column (GE Healthcare) that had been equilibrated with Ni wash buffer (20 mM Tris pH8 ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated at 18°C and submitted to size exclusion chromatography on a HiLoadR 16/600 SuperdexR 200pg or a HiLoadR 16/600 SuperdexR 75pg (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The substrates and products were resolved by 15% urea-denaturing PAGE gel and quantified with Typhoon (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Samples were injected onto either a HiLoad 16/600 Superdex 200 column (GE Healthcare) or a Superdex 200 10/300 GL size-exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... or a Superdex 200 10/300 GL size-exclusion column (GE Healthcare) and purified in SEC buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Samples collected from IMAC were exchanged into size exclusion chromatography (SEC) buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.4) using either a HiPrep 26/10 desalting column (GE Healthcare) or through multiple rounds of concentration and dilution using an Amicon Ultra-15 filter unit with a 10 kDa molecular weight cut-off ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Survivin was purified by immobilized metal ion chromatography (IMAC) with a 5 ml HisTrap FF (GE Healthcare) column followed by overnight dialysis in 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... each histone was subjected to Resource S column chromatography (GE Healthcare). The column was washed with buffer D (20 mM sodium acetate (pH 5.2) ...
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bioRxiv - Biophysics 2022Quote: ... After dialysis the supernatant containing folded tetramers and dimers were subjected to Superose 6 prep grade XK 16/70 size exclusion column (GE Healthcare) purification using HFB buffer ...
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bioRxiv - Biochemistry 2022Quote: ... using a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare Life Sciences, Pittsburg, PA). A 100 µl aliquot of purified atACS protein (4-10 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... using BIA evaluation software 1.0 (GE Healthcare) and plotted in GraphPad Prism 9.0.2 ...
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bioRxiv - Biochemistry 2022Quote: ... followed by GST column purification using the ÄKTA start chromatography system (GE Healthcare, Chicago, IL). Cell lysate supernatant was added to the column containing nickel resin and it was recirculated across the column by a peristaltic pump overnight at 0.5 ml/min ...
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bioRxiv - Biochemistry 2022Quote: Size exclusion chromatography was performed on a HiLoad 16/600 Superdex 200 pg (GE Life Sciences) connected to an AKTA FPLC (GE Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: Ni-NTA eluates were thawed on ice and desalted via the PD-10 desalting column system (GE Healthcare) following the manufacturer’s gravity flow protocol ...
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bioRxiv - Biochemistry 2022Quote: ... connected to an AKTA FPLC (GE Life Sciences) to further purify samples isolated via His-tag purification and buffer swap into a “high salt” buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting soluble fraction was then applied to a Ni-Sepharose column (GE Healthcare Life Sciences) preequilibrated in buffer B (50 mM 3-(N-morpholino ...
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bioRxiv - Biochemistry 2022Quote: ... a volume of 75 µL was injected onto a size exclusion column (Superdex 200-10/300, GE Healthcare) and eluted directly into the SAXS flow-through capillary cell with a flow rate of 0.5 ml.min-1 ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were concentrated and purified on a HiLoad 16/60 Superdex 75 pg (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... 3C protease was removed by Ni-chelated sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...