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Citations for GE Life Sciences :
851 - 900 of 1306 citations for Goat Anti Mouse IgG Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The IgG fraction was isolated on Protein G Sepharose (GE Healthcare 17-0618-02) placed in columns (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... IgGs were isolated through an overnight incubation with Protein G Sepharose (GE Life Sciences) at 4°C ...
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bioRxiv - Immunology 2023Quote: ... or polyclonal IgG affinity-purified with Protein G Sepharose 4 Fast Flow (GE Healthcare) from heat-inactivated and filtered plasma were mixed with 10 TCID50 of SIVmac239 at a 1:1 ratio (5 μl:5 μl ...
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bioRxiv - Immunology 2023Quote: IgG avidity was measured by surface plasmon resonance (SPR; BIAcore 3000, BIAcore/GE Healthcare) to a panel of HIV-1 antigens ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Secondary peroxidase labeled α-mouse (GE Healthcare), α-rabbit (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution ...
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bioRxiv - Immunology 2019Quote: IgG purification from individual rabbit serum samples was performed using Protein G columns (GE Healthcare) according to the manufacturer instructions and adjusted to a final concentration of 20 mg/ml in PBS ...
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bioRxiv - Immunology 2019Quote: ... was filtered and loaded into a HiTrap protein A column (GE Healthcare 17040301, for IgG) or HiTrap KappaSelect column (GE Healthcare Life Sciences – 17545812 ...
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bioRxiv - Cancer Biology 2019Quote: ... IgGs in supernatants were purified through a HiTrap protein A column (GE Healthcare, Chicago, IL). Antibodies were eluted with 0.1M glycine pH 2.7 ...
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bioRxiv - Biochemistry 2022Quote: ... The Fab and IgG proteins were purified from cell supernatants by Ni2+-NTA (GE Healthcare) and protein A affinity chromatography (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared lysate was incubated with 5 ml IgG Sepharose 6 Fast Flow (GE Healthcare) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... IgG was purified from hybridoma culture supernatant through a Protein A-Sepharose column (GE Healthcare), digested with agarose beads conjugated with Ficin (for IgG1 ...
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bioRxiv - Immunology 2020Quote: IgG purification was performed using HiTrap™ Protein G HP (GE Healthcare #17-0404-01) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... α-rabbit IgG secondary antibody conjugated to HRP (from donkey) was purchased from GE Healthcare and used at 1:5,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: Cell lysates were mixed with 400 µl IgG SepharoseTM 6 Fast Flow slurry (GE Healthcare) pre-equilibrated with TNM100 buffer (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Cell Biology 2023Quote: ... in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare). Arrays were hybridized overnight at 37° C at 300 rpm (8 g) ...
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bioRxiv - Biophysics 2020Quote: ... Clarified supernatant was then incubated with 0.5 ml IgG Sepharose 6 FastFlow beads (#17096901, GE Healthcare) for 4 hours ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was poured into a column containing IgG beads (Sepharose 6 Fast Flow, GE Healthcare) and allowed to bind to the beads by gravity-flow ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... The following secondary antibodies were used for immunoblotting: ECL rabbit HRP-linked IgG (GE Healthcare, NA934) and ECL mouse HRP-linked IgG (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Recombinant IgG mAbs were purified using HiTrap Protein A columns (Cytiva/GE Healthcare Life Sciences, 17040303).
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bioRxiv - Immunology 2020Quote: ... The IgG was then purified using protein G affinity chromatography (HiTrap Protein G HP, GE Healthcare) and size-exclusion chromatography (Superdex 200 ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length IgG was obtained by affinity purification utilizing a Protein A chromatography column (GE Healthcare) in AKTA avant (Cytiva) ...
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bioRxiv - Microbiology 2019Quote: ... and IgG was purified on MabSelect SuRe resin (Cat# 17-5438-01; GE Healthcare, Chicago, IL). Other control mAbs used in this study were obtained commercially ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting clarified lysate was batch-bound to ~1 mL of settled IgG sepharose (GE Healthcare) equilibrated in lysis buffer and allowed to incubate on a roller for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Pre-ribosomal particles were purified via immunoglobulin G (IgG) Sepharose 6 Fast Flow beads (GE Healthcare) as described above ...
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bioRxiv - Immunology 2022Quote: ... IgG fractions were further purified by size exclusion chromatography (Superdex 200 Increase, GE Healthcare, Chicago, IL), and Fab fractions were further purified by cation exchange chromatography (MonoS ...
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bioRxiv - Microbiology 2022Quote: ... M28 IgG was then purified from the supernatant using Protein A affinity chromatography (GE Healthcare #17549851). IgG was then dialyzed overnight in TBS (150mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Dynein complexes were captured from Sf9 cell lysates using IgG Sepharose 6 FastFlow beads (GE Healthcare), labeled with SNAP-Cell-TMR-Star ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 3 ml of IgG Sepharose Fast Flow beads (GE Healthcare, 17096901) equilibrated with CMG buffer/300 mM KCl and incubated for 2 hours at 4°C on a rotating wheel ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was harvested and purified after 6 days with protein G sepharose beads (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Fifty microliters of a 50/50 slurry of IgG-Sepharose 6 Fast Flow beads (GE Healthcare) in tandem affinity purification buffer A was added to the supernatant ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary antibody only negative controls established gating for each sample and NeuN labelled nuclei were then sorted and collected in tubes pre-coated with foetal calf serum (FCS; #SH30084.02, Hyclone, GE Healthcare Life Sciences, South Logan, Utah, USA). Purity checks were performed and all samples were validated at >97% purity.
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was then subjected to first round of affinity purification with IgG-SepharoseTM (GE healthcare, Germany) for 1 hour at 4°C on the rotating wheel ...
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bioRxiv - Microbiology 2020Quote: ... while the secondary antibody (1/10,000) was a commercial Amersham ECL-HRP linked rabbit IgG (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified supernatants were then incubated with 150 mL slurry of IgG beads (GE Healthcare, 17-0969-02) for 2h at 4 °C on a turning wheel ...
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Immunology 2023Quote: ... bNAb affinity columns were made by cross-linking IgG to activated CNBr-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were: α-Mouse-HRP (GE Healthcare), α-Rabbit-HRP (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... A plasmid coding for mouse JNK3 cDNA (GE Healthcare) was used as a PCR template and the resultant product was subcloned into mammalian expression vector pRK5 downstream of a myc epitope tag ...