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Citations for GE Life Sciences :
751 - 800 of 1306 citations for Goat Anti Mouse IgG Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Neuroscience 2019Quote: ... and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... The detection of tubulin using mouse anti-tubulin KMX-1 primary (1:100; Gift from Keith Gull, Oxford) and anti-mouse-HRP secondary (1:20,000, GE Healthcare) was used to verify equal sample loading ...
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bioRxiv - Cell Biology 2019Quote: ... we coupled 130 μg of a mouse monoclonal anti-H4K12Ac (gift from Hiroshi Kimura) to 12.5 μl rProtein A Sepharose (GE Healthcare) slurry in antibody coupling buffer (10 mM K-HEPES pH=8 ...
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bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was incubated with pre-washed IgG sepharose (GE Healthcare) and incubated at 4 °C for 3 hours ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Biochemistry 2021Quote: ... The resuspended supernatant was incubated with IgG Sepharose beads (GE healthcare) for binding and followed by incubation with a TEV protease treatment mixture ...
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bioRxiv - Biochemistry 2020Quote: ... 600 μl of IgG Sepharose® 6 Fast Flow (GE healthcare) bead suspension was taken into a column and washed once with 10 ml of IP binding buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Immunology 2021Quote: ... for IgG antibody or LambdaFabSelect Agarose Beads (GE Healthcare Life Sciences) for Fab at 4°C on a rotating shaker overnight ...
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bioRxiv - Immunology 2021Quote: ... specimens were depleted of IgG using Protein G Sepharose (GE Healthcare) as described (41) ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was incubated with IgG Sepharose 6 Fast Flow (GE Healthcare) for 2 h at 4 °C on a nutator ...
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bioRxiv - Biophysics 2020Quote: ... 0.25 mL of IgG sepharose 6 fast flow beads (GE Healthcare) were added to the supernatant and incubated for 1 hour at 4°C while rotating ...
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bioRxiv - Microbiology 2020Quote: ... IgG was purified using a HiTrap Protein A column (GE Healthcare) and Äkta Pure (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Total IgG was purified using Protein G gravitrap kit (GE healthcare). Eluted total IgG was concentrated by centrifugation at 4°C for 30 mins using 100000MWCO vivaspin (Sartorius) ...
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bioRxiv - Immunology 2021Quote: ... and the IgG was purified using protein A Sepharose (GE Healthcare) and eluted with gentle Ag/Ab elution buffer (21027 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL IgG bead suspension (GE Healthcare, cat 17- 0969-01) was washed three times with RP buffer and added to the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... IgG fraction was purified using Protein G Sepharose columns (GE Healthcare) and part of it was labelled with AlexaFluor568 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... and IgG was captured using protein-A affinity chromatography (GE Healthcare). For obtaining the Fab ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL IgG bead suspension (GE Healthcare, cat 17-0969-01) was washed three times with RP buffer and added to the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Neuroscience 2020Quote: ... and developed using an anti-rabbit HRP secondary antibody (1:5000, GE Lifesciences, NA931) and an anti-mouse HRP secondary antibody (1:10,000, GE Healthcare, NA935). Blots were imaged using an Amersham Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Aldolase was detected using the above protocol with 1:10,000 mouse anti-aldolase antiserum (a kind gift from David Sullivan) and 1:2,500 sheep anti-mouse HRP-linked secondary antibodies (GE healthcare, NA931).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Cell Biology 2022Quote: Goat GST antibody (27457701V, GE Healthcare, used at 1:1,000), NeutrAvidin-DyLight 800 (22853 ...
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bioRxiv - Genetics 2020Quote: ... the protein extract was incubated with IgG Fast Flow Sepharose (GE Healthcare) and beads were washed with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was mixed with 10 µL of IgG-agarose (GE Healthcare) and incubated at 4°C overnight on a rotating mixer in the presence of Protease Inhibitor Cocktail (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... IgGs were purified from supernatants by protein A chromatography (GE Life Sciences) followed by SEC using a Superdex 200 Increase 10/300 GL column (GE Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... IgGs were purified by protein A affinity chromatography (MabSelect SURE, GE Healthcare) [18].
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bioRxiv - Cell Biology 2022Quote: ... Proteins were immunoprecipitated by IgG Sepharose beads (GE Healthcare; 17-0969-01) (for Lid1-TAP ...
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bioRxiv - Microbiology 2020Quote: ... 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934), 1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... Sepharose 6B purified cell lysate was incubated with IgG Sepharose (GE Healthcare) followed by buffer wash steps to remove non-specifically binding proteins ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Polyclonal IgG was purified from plasma using Protein A resin (GE Healthcare) and digested into Fabs using immobilized papain resin as described previously46 ...
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bioRxiv - Immunology 2023Quote: ... IgGs were purified by Protein G Sepharose 4 Fast Flow (GE Healthcare) and concentrated by Amicon Ultra 4 ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were bound to IgG Sepharose 6 Fast-Flow (GE Healthcare) via the ZZ tag on the heavy chain ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were incubated with IgG Sepharose 6 Fast-Flow (GE Healthcare) for four hours at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were immunoprecipitated by IgG Sepharose beads (GE Healthcare; 17-0969-01) (for Lid1-TAP ...