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Citations for GE Life Sciences :
801 - 850 of 1306 citations for Goat Anti Mouse IgG Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... The Anti-hIgG Fc (AHC) capture biosensors were purchased from ForteBio (California, USA) and the protein A coated chips were purchased from GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Germany) were grown in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS, GE Healthcare, Freiburg, Germany or Merck) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble extract cleared of cellular debris was incubated with IgG sephorose (GE Healthcare) for 2h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2020Quote: ... The IP was conducted by adding 20µL of IgG sepharose beads (GE Healthcare) to each IP sample for a 4-hour incubation at 4◦C ...
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bioRxiv - Cell Biology 2019Quote: ... Augmin holocomplex was affinity-purified using IgG-Sepharose (GE Healthcare, 17-0969-01) and eluted via cleavage with 100–200 µg of GST-HRV3C protease ...
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... 500μL total protein was incubated with 20μL IgG-Sepharose 6 resin (GE healthcare) for 2 hrs at 4°C with rotation ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). For IgA1 or IgA2 antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with IgG Sepharose beads (GE Healthcare Life Sciences) for 1 hr ...
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified supernatant was incubated with IgG sepharose beads (GE Healthcare Life Sciences) for 1.5 hours at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated with 1 ml of IgG Sepharose resin (GE Healthcare) for 1 hr ...
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bioRxiv - Cell Biology 2022Quote: ... The IgG fractions of Rb80 or Rb54 were obtained by ProteinA (GE healthcare) affinity chromatography ...
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bioRxiv - Molecular Biology 2023Quote: ... along with corresponding species IgG secondary antibodies (1:5000, GE Healthcare, Fisher Scientific) were utilised ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). To produce antibody Fabs ...
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bioRxiv - Immunology 2024Quote: ... supernatants were harvested and IgG was purified on Protein G Sepharose (GE Healthcare)
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bioRxiv - Biophysics 2023Quote: ... and the supernatant was incubated with 1 mL IgG Sepharose beads (GE Healthcare) for 1 h at 4 ℃ ...
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bioRxiv - Microbiology 2023Quote: ... Cleared lysate was incubated with washed IgG Sepharose 6 Fast-Flow (GE Healthcare) for 3 hours at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-coupled α-mouse (GE Healthcare) 1:10000 ...
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bioRxiv - Microbiology 2020Quote: Binding kinetics and affinities for ACE2.Fc were assessed using surface plasmon resonance technology on a Biacore T200 instrument (GE Healthcare, Marlborough, MA) using a Series S CM5 sensor chip in filtered and degassed HBS-EP running buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked antirabbit immunoglobulin G (both Cytiva, formerly known as GE Healthcare Bio-Sciences NA931 and NA934), Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Bioengineering 2022Quote: ... and IgG was purified with Protein A sepharose beads (GE Healthcare 17-5280-04).
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bioRxiv - Bioengineering 2021Quote: ... and IgG was purified with Protein A sepharose beads (GE Healthcare 17-5280-04).
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bioRxiv - Physiology 2021Quote: ... probed with primary antibodies followed by secondary horseradish peroxidase (HRP)-conjugated IgG (GE Healthcare), and finally visualized by enhanced chemiluminescence (Western Lightning ECL-Pro ...
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bioRxiv - Microbiology 2019Quote: ... IgG antibodies were purified from the supernatant using a Protein G column (GE Healthcare). The subclass of each IgG antibody was determined using a human IgG subclass ELISA kit (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... Supernatants were concentrated and IgG was purified using Ab Spintrap columns (GE Healthcare/Cytiva).
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bioRxiv - Microbiology 2022Quote: ... IgG was isolated using 5 ml HiTrap Protein G High Performance column (GE Healthcare), whereas IgM was isolated using POROS™ CaptureSelect™ IgM Affinity Matrix (ThermoScientific ...
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bioRxiv - Immunology 2020Quote: ... IgG was purified with a HiTrap MabSelect SuRe protein A affinity column (GE Healthcare) on an AKTA Pure (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Fabs and IgGs were purified from transfected cell supernatants using Ni-NTA (GE Healthcare) (for Fabs ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare) except for Fig ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare). The concentration of purified IgG was measured at OD280.
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bioRxiv - Cell Biology 2021Quote: ... IgG purified protein was further subjected to affinity purification using Calmodulin Sepharose (GE Healthcare). Purified TRAPPII was further subjected to size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... IgG antibodies were purified from rabbit serum using protein A sepharose beads (GE Healthcare), and these IgG antibodies were administered to brain ischemia model mice as a control antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used were rabbit IgG HRP conjugated (GE Healthcare life sciences, Malborough, MA) or mouse IgG HRP conjugated (GE Healthcare life sciences ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were incubated with immunoglobulin G (IgG) Sepharose 6 Fast Flow beads (GE Healthcare) on a rotating wheel at 4 °C for 90 min ...
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bioRxiv - Biophysics 2022Quote: ... The elution was incubated with 1ml IgG Sepharose 6 Fast Flow resin (GE healthcare) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was incubated with 0.7 ml of IgG Sepharose beads (GE Healthcare) at 4°C for 3.5 hr ...