Labshake search
Citations for GE Life Sciences :
7951 - 8000 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... equilibrated with buffer A using a ÄKTA Pure system (GE Healthcare). LF and its mutants were eluted by a linear NaCl gradient using buffer A and 20 mM HEPES pH 7.2 ...
-
bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein was loaded onto a Q column (GE Healthcare) pre-equilibrated with low salt buffer (25 mM K.HEPES pH 8 ...
-
bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (FBS) (HyClone, GE Life Sciences) and penicillin-streptomycin solution (P/S ...
-
bioRxiv - Biophysics 2023Quote: ... concentrated and subjected to size exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 (GE Healthcare) column ...
-
bioRxiv - Bioengineering 2023Quote: ... Further purification was done in 96 well microplates using Capto Q AEX-resin (GE Healthcare).
-
bioRxiv - Bioengineering 2023Quote: ... and ImageQuant LAS 4000 Mini (GE Healthcare). All probes were synthesized by PCR using DIG DNA Labeling Mix ...
-
bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
-
bioRxiv - Biochemistry 2023Quote: ... the lysate was incubated for 45 minutes with 20 µl of Ni Sepharose 6 Fast Flow beads (GE Healthcare), washed and resuspended in 100 µl of buffer A (30 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... then imaged on a Typhoon imager (GE Healthcare) with 495 excitation and 520 emission wavelengths ...
-
bioRxiv - Biochemistry 2023Quote: ... and after extensive washing in PBST the dried membrane was scanned with an imaging system (GE Healthcare Amersham Typhoon). For lectins ...
-
bioRxiv - Biochemistry 2023Quote: ... proteins were purified by nickel affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 20 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Biochemistry 2023Quote: ... The SEC analyses were performed with a Superose 6 increase 10/300 column (GE Healthcare, USA) or a Superdex 200 increase 10/300 column (GE Healthcare ...
-
Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with IgG Sepharose beads (GE Healthcare) on a rotary wheel for 1 h at 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... A MicroCal iTC200 system (GE Healthcare) was used to conduct the ITC measurements ...
-
bioRxiv - Bioengineering 2023Quote: ... All scans were acquired on a 1.5 T GE scanner (Signa HDxt, GE Healthcare, United States). Each subject underwent three scans in orthogonal planes using the single-shot Fast Spin-Echo (ssFSE ...
-
bioRxiv - Biochemistry 2023Quote: ... for the PWWP domain or a HiTrap Q HP column (GE Healthcare) for the ADD domain ...
-
bioRxiv - Biochemistry 2023Quote: ... and further purification through size-exclusion chromatography on a Hiload 16/600 Superdex 200 column (GE Healthcare) in buffer containing 20 mM Hepes-NaOH (pH 7.2) ...
-
bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
-
bioRxiv - Biochemistry 2023Quote: ... or a Superdex 200 increase 10/300 column (GE Healthcare, USA) in the buffer containing 25 mM MES pH 5.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Free copper was then removed by passing the protein through a HiTrap Desalting column (GE Healthcare). Prior to the SANS experiments ...
-
bioRxiv - Immunology 2023Quote: The binding kinetics and affinity of monoclonal antibodies to the SARS-CoV-2 spike trimer were analyzed by SPR (Biacore T100, GE Healthcare). Specifically ...
-
bioRxiv - Biochemistry 2023Quote: ... Desired fractions were incubated with 0.5 mM FeCl3 in presence of 200 mM NaHCO3 for 1h at room temperature and further purified SEC on a S200 16/600 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.2 ...
-
bioRxiv - Immunology 2023Quote: ... The sensograms were fit in a two-component model with BIA Evaluation software (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... 1.8 µl of nickel magnetic Sepharose beads (28-9799-17, GE Healthcare; pre-blocked with 25 mM Tris-HCL ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered at 0.8 µm and used for purification by nickel ion affinity chromatography (GE Healthcare). A washing step in the lysis buffer supplemented with 30 mM imidazole was followed by the elution in lysis buffer supplemented with 500 mM imidazole ...
-
bioRxiv - Biochemistry 2023Quote: ... loaded on a 1 ml heparin column (GE Healthcare) and eluted in the heparin buffer supplemented with 500 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was passed through a HiLoad® 26/600 Superdex® 75 pg (GE Healthcare) size exclusion chromatography column and the resulting pure protein was stored in gel filtration buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... a final gel filtration step was performed in GF buffer (30 mM HEPES pH 8, 250 mM NaCl, 10 mM TCEP) using a S200 size exclusion chromatography column (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated Hsp10 preps were first diluted tenfold with chromatography buffer before loading into a HiPrep SP XL 16/10 column (GE Healthcare). A sodium chloride linear gradient from 0 to 1 M was used to purify Hsp10 and it eluted at a conductivity of 13.2 mS/cm ...
-
bioRxiv - Biochemistry 2023Quote: ... Hsp10 fractions were pooled and concentrated using a Vivaspin 20 MWCO 10,000 spin column before purification on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
-
bioRxiv - Biochemistry 2023Quote: ... and deletion mutant preps were first diluted tenfold with chromatography buffer before being loaded through a Hi Trap Q XL column (GE Healthcare). A linear gradient of sodium chloride from 0 to 1 M was used to purify the chaperonins where each eluted at a conductivity of 5.5 mS/cm ...
-
bioRxiv - Biochemistry 2023Quote: ... The evaporated solution was centrifuged as described above and applied to a QAE Sephadex A25 column (GE Healthcare) equilibrated with 500 ml of 50 mM Tris/HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
-
bioRxiv - Biochemistry 2023Quote: ... ion exchange chromatography using a HiTrap Heparin HP column (GE Healthcare) and further purification through size-exclusion chromatography on a Hiload 16/600 Superdex 200 column (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... and size-exclusion chromatography using a Hiload 16/600 Superdex 75 column (GE Healthcare). The purified protein sample was stored at -80 °C before use.
-
Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superose 6 Increase size exclusion chromatography column (10/300 prep grade, GE Healthcare). Elution was carried out with 150 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... ion exchange chromatography using a HiTrap Heparin HP column (GE Healthcare) for the PWWP domain or a HiTrap Q HP column (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by size-exclusion chromatography to remove over-crosslinked aggregation on a Superose 6 increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM Tris-HCl (pH 7.2) ...
-
bioRxiv - Bioengineering 2023Quote: ... 13 fetuses at 23w to 37w GA scanned on a 1.5 T GE scanner (Signa HDxt, GE Healthcare, United States) were used for the following analysis ...
-
bioRxiv - Biochemistry 2023Quote: ... The purified EGFR recombinant extracellular domain was first coupled to the surface of a CM5 sensor chip (GE Healthcare Biosciences)) ...
-
bioRxiv - Biochemistry 2023Quote: ... except that purification by size exclusion was omitted and the solubilized inclusion bodies were pre-cleaned by passage over a Hi-Trap Q-Sepharose column (GE Healthcare) before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare) ...
-
bioRxiv - Cell Biology 2023Quote: ... The environmental controller of a DeltaVision OMX Flex imaging station (GE Healthcare-Applied Precision) was set to heat the stage and objective lens at 37°C and allowed to equilibrate for >30 mins ...
-
bioRxiv - Cell Biology 2023Quote: ... image stacks were collected at z-increments of 0.125 µm using a DeltaVision OMX Flex imaging station (GE Healthcare-Applied Precision). A 60x oil immersion lens (numerical aperture [NA] 1.42 ...
-
bioRxiv - Biophysics 2023Quote: ... supernatant was incubated with Ni Sepharose (GE Healthcare), and the bound protein was eluted with buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
-
bioRxiv - Biochemistry 2023Quote: ... Lysate was circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged with 100 mM CoCl2 for 2 hrs ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatant was circulated over 5 mL HiTrap chelating column (GE Healthcare, Cat# 17-0409-01) that was pre-incubated with 100 mM CoCl2 for 10 minutes ...