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Citations for GE Life Sciences :
8201 - 8250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed proteins were loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare) and eluted with a linear gradient of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted fraction was concentrated and further purified by size exclusion chromatography using an S200 increase (10/300 GL) column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT in the presence of TEV protease and subsequently purified using a 5 mL HiTrap HP Heparin column (GE Healthcare), eluting with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted fraction was concentrated and further purified by size exclusion chromatography using a Superdex (16/600) column (GE Healthcare) in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... and further purified by size exclusion chromatography using an Superdex (16/600) column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved CHD4-C1bC2a was further purified by size exclusion chromatography on a Superdex 75 10/300GL column (GE Healthcare) and eluted with 20 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... Dialysed protein was passed through a 5 mL MBPTrap column (GE Healthcare). The flow-through fraction was concentrated and further purified by size exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified using a 5 mL HiTrap HP Heparin column (GE Healthcare) and eluted with a buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and further purified by size exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was applied to a HisTrap affinity column (GE Healthcare) equilibrated in lysate buffer and eluted using a gradient against lysate buffer containing 500 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: Purified protein or protein complex (>1.5 mg/mL) was subject to a 24 mL Superdex 200 analytical gel filtrating column 10/300 GL (GE healthcare) at a rate of 0.5 mL/min at 20 °C by HPLC (Varian) ...
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bioRxiv - Biochemistry 2022Quote: ... The biotinylated PCR products were purified on a MiniQ 4.6/50 ion exchange column (GE Healthcare Life Sciences) and loaded into a HiTrap Streptavidin HP column (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2022Quote: ... the complex was centrifuged at 17,000 xg for 10 minutes and then loaded onto a Superose 6 (GE Healthcare) column equilibrated in wash buffer containing 25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... RAD51 without the his-SUMO tag was then recovered into the flow thru and directly loaded onto a HiTrap heparin column (GE Healthcare). The column was washed with W2 buffer and then a 0.1-1M NaCl gradient was applied ...
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bioRxiv - Biochemistry 2022Quote: ... and a RESOURCE™ Q column (GE Healthcare). GST-tagged GTPases were purified on high affinity GST resin (GenScript) ...
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bioRxiv - Biochemistry 2022Quote: ... SEC was carried out on a Superose 6 Increase 10/300 GL column by fast protein liquid chromatography (ÄKTA; GE Healthcare); 0.25 mM fractions were collected ...
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bioRxiv - Biochemistry 2022Quote: ... DOCKDHR2 domains were further purified by a HiLoad 26/60 Superdex 75 gel filtration column (GE Healthcare) and a RESOURCE™ Q column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2022Quote: ... donkey anti-rabbit IgG-HRP (1:5000, #NA9340, GE Healthcare). Detection ...
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bioRxiv - Cancer Biology 2022Quote: ... and the resultant [18F]RL-III-5F7 conjugate was isolated by gel filtration over a PD10 column (GE Healthcare, Piscataway, NJ) eluted with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... and then separating the complex from free GTPase by a HiLoad 26/60 Superdex 200 gel filtration column (GE Healthcare), followed by a RESOURCE™ Q column ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies anti-IgG (anti-rabbit or anti-mouse, GE Healthcare, 1:5,000) conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Bioengineering 2022Quote: Equilibrium dissociation constants were determined by surface plasmon resonance using a Biacore T200 instrument (GE Healthcare). Approximately 100 resonance units (RU ...
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bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
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bioRxiv - Cancer Biology 2022Quote: ... and electrotransferred to 0.2 µm nitrocellulose membranes (GE Healthcare). After blocking in Tris-buffered saline with 0.2% Tween and 5% bovine serum albumin ...
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bioRxiv - Bioengineering 2022Quote: ... and then by size exclusion chromatography (SEC) (Superdex S-200preparatory-grade column (GE Healthcare)) ...
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bioRxiv - Bioengineering 2022Quote: ... pH 7.4) onto a Superdex 200 column (GE Healthcare) at a flow rate of 0.5 mL/min using an AKTA Pure FPLC machine ...
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bioRxiv - Bioengineering 2022Quote: ... we loaded the supernatants onto a 5 mL HisTrap HP column (GE Healthcare) using an AKTA Pure FPLC machine ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Bioengineering 2022Quote: ... We further purified the proteins through a 25 mL Superdex 200 preparatory-grade column (GE Healthcare) with 20 mM Na2HPO4 and 100 mM NaCl (pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... The OD600 was measured periodically using the spectrophotometer Ultrospec 500pro (GE Healthcare Life Sciences, USA).
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Bioengineering 2022Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated with Ficoll-Paque Plus (GE Healthcare, Chicago, IL) from human blood ...
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bioRxiv - Bioengineering 2022Quote: ... a 30% isotonic Percoll (GE Healthcare), underlaid with 70% isotonic Percoll and spun at 650 G for 25 minutes with no brake separated red blood cells ...
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bioRxiv - Bioengineering 2022Quote: ... Protein purification was either carried out by gravity flow Ni NTA Superflow columns (Iba) or FPLC using a His-TrapFF 1ml column (GE Healthcare, Munich, Germany). Concentrated protein eluates were stored at 4°C.
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bioRxiv - Bioengineering 2022Quote: ... and the supernatant containing the soluble fraction was collected and filtered sterilized before purification in the ÄKTA Pure chromatograph (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... and loaded on a preparative Superdex200 increase 10/300 GL (GE Healthcare) column for size exclusion chromatography using a running buffer containing 20 mM Tris-HCl ...
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bioRxiv - Bioengineering 2022Quote: ... and Neq2X7 were purified by affinity chromatography using a 1ml HisTrap™HP on an ÄKTA Pure chromatography system (GE Healthcare). After washing of the column ...
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bioRxiv - Bioengineering 2022Quote: ... followed by in-gel fluorescent scanning using the Typhoon FLA 9500 (GE Healthcare) and then the Coomassie brilliant blue staining ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was cleared by centrifugation and the supernatant (SN) incubated for 1.5 h with Glutathione Sepharose 4B (GE Healthcare), equilibrated in buffer A (25 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... and then loaded onto a Superose 6 increase column (GE Healthcare) on an NGC system (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was loaded onto a 5mL His Trap HP column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biophysics 2022Quote: ... Protein concentration of the extracts were determined by SDS-PAGE and densitometry analysis using the LMW-SDS Marker Kit (GE Healthcare) as standard.
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bioRxiv - Cancer Biology 2022Quote: ... was used for separation of proteins which were transferred to nitrocellulose blotting membrane (GE Healthcare, #10600002). Following washing with 0.05% Tris buffered saline-Tween (TBST) ...
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bioRxiv - Cancer Biology 2022Quote: ... ovalbumin (44 kD) and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were photographed with the IN Cell Analyzer 2200 high content analyzer (GE Healthcare), using a 10x objective ...
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bioRxiv - Cancer Biology 2022Quote: ... For BrdU staining (1:500, #RPN20AB, GE Healthcare) cells were fixed with ice-cold methanol (40 sec ...
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bioRxiv - Cell Biology 2022Quote: ... before subjecting it to SEC (Superose 6 column, GE healthcare). For labeling purified KASH5 used in TIRF experiments ...
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bioRxiv - Cell Biology 2022Quote: ... 500 µg of the purified protein or the complex was loaded on a Superdex 200 10/300 size exclusion column (GE Healthcare) in line with both a DAWN HELIOS II MALS detector (Wyatt Technology ...
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bioRxiv - Bioengineering 2022Quote: ... cultured cells were fixed with 4% PFA in PBS for 20 min at room temperature (RT) and permeabilized and blocked with 0.1% Triton X-100 and 10% fetal bovine (FBS; GE Healthcare Life Science, Marlborough, MA) in PBS for 30 min at RT ...