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Citations for GE Life Sciences :
8101 - 8150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and run on a 16/600 S200 column (GE healthcare) pre-equilibrated in 50 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed sample was then loaded onto a 10 mL Mono Q column (GE healthcare) pre-equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2022Quote: ... pooled and concentrated to 10 ml and applied to a 320 ml size exclusion column (HiLoad ® 26/600 Superdex ® 200, GE Healthcare). The column was equilibrated with 30 mM PIPES pH 7.4 buffer containing 100 mM NaCl + 1 mM TCEP+ 15 μM PLP and eluted with the same buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions of 8 ml were collected in an automated AKTA FPLC system (GE Healthcare) and purity was determined with SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was passed through a 5 ml Ni2+ affinity column (GE Healthcare) and eluted with the same lysis buffer containing imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions from the Streptactin column were applied to a Superdex 200 26/60 size exclusion column (GE Healthcare) and eluted in Streptactin binding buffer.
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bioRxiv - Biophysics 2022Quote: ... The collected peak fraction was further purified through a HiTrap desalting column (GE Healthcare) to exchange the buffer (20 mM potassium phosphate ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was purified with the His-tag affinity by chromatography with a HisTrap HP Ni2+sepharose column (GE Healthcare). The collected peak fraction was further purified through a HiTrap desalting column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... and data was developed using an Amersham Imager 600 (GE Healthcare) or standard film techniques ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant WT and mutant IN molecules were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... The homogenate was clarified by centrifugation and mixed with Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL). After extensive washing ...
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bioRxiv - Immunology 2022Quote: ... Buffer exchange to PBS was performed before size exclusion chromatography (Äkta Avant, GE Healthcare) with a SuperdexTM 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... and biotinylated proteins were immunopurified through Streptavidin-Mag Sepharose™ (GE Healthcare). After washing of the beads ...
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bioRxiv - Developmental Biology 2022Quote: ... in presence of 32P end-labeled telomeric primers that has been purified using a micro-spin G-25 column (GE healthcare). PCR reactions were resolved by 9% polyacrylamide gel electrophoresis at room temperature ...
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bioRxiv - Microbiology 2022Quote: GST-tagged protein was purified using Glutathione-based affinity chromatography (GSTrap FF GE Healthcare). Cells were thawed on ice and resuspended in Buffer F (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Hexa-histidine tagged protein was purified using Nickle-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta prime FPLC system ...
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bioRxiv - Microbiology 2022Quote: ... and further purified by size exclusion chromatography (HiLoad 16/600 Superdex 200 GE Healthcare), pre-equilibrated with buffer E ...
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bioRxiv - Microbiology 2022Quote: ... 1mM b-ME) and further purified by Size Exclusion Chromatography (HiLoad 26/600 Superdex 200, GE Healthcare), pre-equilibrated with buffer E (20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... K11-Ub4 was made from K11-Ub2 and purified by size exclusion chromatography using a Superdex 75 HiLoad 16/600 column (GE Healthcare). The yield for K11-Ub4 was about 10% ...
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bioRxiv - Biophysics 2022Quote: ... the protein was eluted by gravity flow using a PD MiniTrapTM G-25 (GE Healthcare) Sephadex column into 200 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysates were loaded first with 2 ml of DEAE Sepharose fast flow (GE Healthcare) and the elutes obtained by a gradient of 100-500 mM NaCl in 50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2022Quote: ... using Ni-IMAC (HisTrap HP, GE Healthcare) followed by gel filtration (HiLoad 16/60 Superdex 75 or HiLoad 16/60 Superdex 100 ...
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bioRxiv - Biochemistry 2022Quote: ... followed by gel filtration (HiLoad 16/60 Superdex 75 or HiLoad 16/60 Superdex 100, GE Healthcare) as previously described [38] ...
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bioRxiv - Biochemistry 2022Quote: ... Size-exclusion chromatography was performed on a 24 ml Superdex-75 gel filtration column on an ÄKTA Purifier System (GE Healthcare, Chicago, Illinois, USA) using the same buffer as during sedimentation velocity measurement ...
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bioRxiv - Biophysics 2022Quote: ... and the ÄKTAprime plus system (GE Healthcare). The bound proteins were eluted with 250 mM NaCL ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The sample was further purified via size-exclusion chromatography (SEC) with a HiLoad 16/600 75 pg (GE Healthcare) column in buffer containing 150 mM NaCL ...
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bioRxiv - Biophysics 2022Quote: ... supplemented with 10% (vol/vol) FBS (GE Healthcare Life Sciences), 1% nonessential amino acid and 10mM HEPES at 37 °C with 5% (vol/vol ...
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bioRxiv - Immunology 2022Quote: ... The complex was purified from excess/unassembled material using size-exclusion chromatography (HiLoad® 16/600 Superdex® pg200 (GE Healthcare) column running TBS buffer (Alfa Aesar)) ...
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bioRxiv - Immunology 2022Quote: ... using Vivaspin filters with a 50,000 kDa molecular weight cutoff (GE Healthcare). GPC-I53-50A for rabbit immunization studies were subjected to an additional SEC step using a Superose 6 increase 10/300 GL column in TBS ...
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bioRxiv - Immunology 2022Quote: ... GPC-I53-50A was concentrated to at least 2 µg/ml using Vivaspin centrifugal filters (GE Healthcare). To generate complexes ...
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bioRxiv - Immunology 2022Quote: ... Fractions between 8.5 and 10 mL were pooled and concentrated by centrifugation at 350 x g using Vivaspin filters with a 10,000 kDa molecular weight cutoff (GE Healthcare). Concentrated GPC-I53-50NPs were then diluted 1:1 with TBS ...
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bioRxiv - Immunology 2022Quote: ... pooled and concentrated using Vivaspin filters with a 50,000 kDa molecular weight cutoff (GE Healthcare). The Nanodrop was used to determine the concentrations of expressed proteins applying the proteins peptidic molecular weight and extinction coefficient as calculated by the online ExPASy software (ProtParam).
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bioRxiv - Immunology 2022Quote: ... Trimeric spike protein was further purified by Superose 6 size-exclusion chromatography (GE Healthcare) in 10 mM Tris pH 8 ...
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bioRxiv - Immunology 2022Quote: ... The antibodies were purified from Expi293 cell supernatant by high-performance liquid chromatography (HPLC) coupled with HiTrap Protein A HP columns (Cytiva, formerly GE Healthcare). Monoclonal antibodies were then dialyzed by SnakeSkin dialysis tubing (10K MWCO ...
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2022Quote: ... The complex was purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... and the resulting data were fitted to a 1:1 binding model using BIAcore 8K Evaluation Software (v3.0.12.15655; GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was filtered through a 0.2µm cellulose filter (GE Life Sciences). Lysate was applied to an equilibrated HisTrap (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... peroxidase-conjugated anti-mouse-IgG antibody (GE Healthcare, Chicago, IL, USA), diluted 1:5,000 in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... IBA) followed by size exclusion chromatography (SEC; HiLoad 26/600 Superdex 200 pg column, GE Healthcare). Both proteins were mixed in a molecular ratio 1:1 and the AR3C-HC84.1 Fab mixture was re-purified by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Both proteins were mixed in a molecular ratio 1:1 and the AR3C-HC84.1 Fab mixture was re-purified by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) with PBS as the eluent.
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bioRxiv - Microbiology 2022Quote: ... Plaque assay analysis was performed using ImageQuant TL 8.2 software (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
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bioRxiv - Microbiology 2022Quote: ... The histidine-tagged quail C8γ and OmCI were produced and purified by Ni-NTA affinity chromatography according to the manufacturer’s instructions (GE Healthcare, Piscataway, NJ). Antisera against quail C8γ were generated by immunizing four-week-old Swiss Webster mice with the recombinant quail C8γ as described (Benoit et al ...
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bioRxiv - Biophysics 2022Quote: ... and loaded onto a Superose 6 increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed thrice in PBS and plates were scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 25 μm; GE Healthcare). All staining steps were performed at room temperature for one hour ...
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bioRxiv - Biophysics 2022Quote: ... Cy5 (GE Healthcare) labeled microtubules ...
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bioRxiv - Biophysics 2022Quote: ... Ni-NTA affinity chromatography was performed using a 1mL Ni-NTA cartridge (GE Healthcare). The protein was eluted with buffer containing 20 mM Tris·HCl ...