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Citations for GE Life Sciences :
701 - 750 of 1756 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Biotinylated membrane proteins were purified using Streptavidin High Performance Spintrap columns (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Mononuclear cells from bone marrow samples were isolated using Ficoll-Paque (GE Healthcare, Chicago, IL, USA) density gradient centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Il USA). Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... and imaged by a GE IN Cell Analyzer 2000 imaging system (GE Healthcare, Chicago, IL, USA). For the staining ...
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bioRxiv - Microbiology 2019Quote: ... and IgG was purified on MabSelect SuRe resin (Cat# 17-5438-01; GE Healthcare, Chicago, IL). Other control mAbs used in this study were obtained commercially ...
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bioRxiv - Immunology 2021Quote: ... which was underlayed with 4 ml 80% percoll solution (GE Healthcare-Life Sciences, Chicago, IL, USA). Tubes were centrifuged (1600 G ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was collected and fractionated on a HiTrap Q column (GE Healthcare; Chicago, IL) equilibrated in 20 mM Tris ...
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bioRxiv - Biophysics 2022Quote: SEC analysis was performed with Superose 6 increase 10/300GL column (GE Healthcare, Chicago, IL, USA). Flow rate was set at 0.75 mL/min ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were immediately placed on the platen of a DeltaVision Elite microscope (GE Healthcare, Chicago, IL). Time-lapse videos were taken every 30 sec for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... the cell suspension was loaded on a Percoll (GE Healthcare, Chicago, IL, cat#: 17-0891-01,) gradient of 35% and 65% and centrifuged at 1800×g for 10min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... IgG fractions were further purified by size exclusion chromatography (Superdex 200 Increase, GE Healthcare, Chicago, IL), and Fab fractions were further purified by cation exchange chromatography (MonoS ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified from cell extracts using MBPTrap™ HP column (GE Healthcare, Chicago, IL, USA) with ÄKTA FPLC system (Amersham Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Purification was performed using a size-exclusion chromatography column (PD-10; GE Healthcare, Chicago, IL, USA) and eluted with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were further purified via gel filtration (Superdex 200, GE Healthcare Chicago, IL, USA). In all cases ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were further purified via gel filtration (Superdex 200, GE Healthcare Chicago, IL, USA). In all cases ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Neuroscience 2023Quote: ... and detected with the Amersham Imager 600 imaging system (GE Healthcare Life Sciences, Chicago, IL, USA). The images were analyzed with Image Lab software (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were concentrated using the 2-D Clean-Up Kit (80648451, GE Healthcare, Chicago, IL), reduced in 1 × Laemmli’s sample buffer (32.9 mM Tris HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatants were initially applied to a HisGraviTrap column (GE Healthcare Life Sciences, Chicago, IL, USA), which was pre-equilibrated with HisTrap binding buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA was blotted onto a nylon membrane (Amersham Hybond-N+; GE Healthcare, Chicago, IL, USA) and hybridized with DIG-labeled probe using DIG Easy Hyb (Roche Diagnostics ...
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bioRxiv - Microbiology 2023Quote: The concentration of lung RNA was quantified using a Nanodrop (NanoVue Plus, GE Healthcare, Chicago, IL). 400ng of RNA was converted to cDNA using the Qiagen RT2 First Strand Kit (330404 ...
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bioRxiv - Biochemistry 2024Quote: ... The Enhanced chemiluminescence (ECL) solutions for the Western blotting system were from GE Healthcare (Chicago, IL). The protease and phosphatase inhibitor cocktails were purchased from Sigma-Aldrich.
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bioRxiv - Biochemistry 2023Quote: ... The supernatants were initially applied to a HisGraviTrap column (GE Healthcare Life Sciences, Chicago, IL, USA), which was pre-equilibrated with HisTrap binding buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal was developed using Amersham ECL Prime Western blotting Detection reagent (GE Healthcare, Chicago, IL, USA), and images taken using a G:BOX gel imaging system (Syngene ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantified by Amersham Imager 600 imaging system (AI600, GE Healthcare Life Sciences, Chicago, IL, USA).
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The clear supernatant layer was collected and run over five serially connected Hi-Trap desalting columns (GE healthcare, 17-1408-01) in buffer A supplemented with 2 mM DTT and 14% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... Further purification was carried out by size exclusion chromatography using a Superdex™ 200 Hi Load 16/60 column (GE Healthcare). Proteins were eluted with SEC buffer (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysed cells were centrifuged 20,000 xg for 30 minutes at 4C and soluble lysate was filtered prior to IMAC purification using 2×5mL His-Trap columns (GE Healthcare) affixed to an Akta Pure ...
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bioRxiv - Molecular Biology 2020Quote: ... the buffer was supplemented with 30 mM imidazole and an additional 100 mM NaCl and passed through a 1 ml Hi-Trap Chelating column (GE Healthcare) to capture the N-terminal fragment that was cleaved by the TEV protease ...
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bioRxiv - Biophysics 2019Quote: ... Sample was further purified from free labelled peptide and Sortase by size-exclusion chromatography using a Hi/Load 16/600 Superdex 75 column (GE Healthcare) previously equilibrated with ZapA storage buffer ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... After cell lysis by sonication cell debris was removed by centrifugation and the lysate was passed through a His-Trap column (GE Healthcare). Proteins were eluted with a gradient of buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... except that purification by size exclusion was omitted and the solubilized inclusion bodies were pre-cleaned by passage over a Hi-Trap Q-Sepharose column (GE healthcare) before loading onto a Hi-Trap SP-Sepharose column (GE healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 concentrators and further separated by HiTrap Heparin HP column and Hi-load 16/600 superdex 200 column (GE Healthcare). Fractions from the column were pooled and subjected for SEC-MALS analysis to determine their oligomeric status ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted proteins were then treated overnight with recombinant HRV 3C protease to remove the N-terminal His-tag and further purified via size exclusion chromatography on a HiLoad 26/600 Superdex 75 column (GE Healthcare) operating with 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... The liposomes were then purified by gel filtration using a Hi Prep 16/60 Sephacryl S-300 HR column (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed 6×His tagged ARL3 and its variants were purified with Ni SepharoseTM 6 Fast Flow beads (GE Healthcare) and cleaved with thrombin (Solarbio ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare) again ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...