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Citations for GE Life Sciences :
651 - 700 of 1756 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: The purified rhGFAT2-his protein was subjected to a size exclusion chromatography using a Superdex 200 column (GE Healthcare, USA). The column was equilibrated with 1 CV of 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was filtered through a 0.22-μm filter and loaded onto a 5 mL His Trap HP column (GE Healthcare) pre-equilibrated with buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by size exclusion chromatography (Hi-load 16/60 Superdex 200 column, GE Healthcare Life Sciences) in a buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was first subjected to immobilized metal affinity chromatography (IMAC) using a 5mL Hi-Trap TALON column (GE healthcare sciences). The running buffer for IMAC was buffer TA ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... Recombinant His-tagged proteins (MA4376, MA4376D54N and MA4375) were purified with the ÄKTAprime plus system using HisTrap HP (GE healthcare) columns ...
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bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
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bioRxiv - Microbiology 2022Quote: ... We pelleted and lysed bacteria by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 0.5 M imidazole ...
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bioRxiv - Microbiology 2023Quote: ... Pooled His-A51R factions were run over a Superdex 200 Increase 10/300 size-exclusion chromatography (SEC) column (GE Healthcare), exchanged into Storage Buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The myosin was purified using His-tag affinity purification and anion-exchange purification by the AKTA purify system (GE Healthcare). Oligonucleotide labeling reactions were performed just after anion-exchange purification ...
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bioRxiv - Biochemistry 2023Quote: ... Both anti-His and anti-Strep immunoblots were developed using Amersham ECL immunoblotting reagents (GE Healthcare Life Sciences, Buckinghamshire, UK).
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bioRxiv - Cell Biology 2022Quote: ... the lysate was centrifuged at 45,000 g for 30 min and His-(MBP-) ZNF524 further purified on a HisTrapTM HP (GE Healthcare) via the Akta Prime Plus System ...
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bioRxiv - Cell Biology 2023Quote: Both the GST-bait and His-prey lysates were incubated with Glutathione Sepharose 4B (GE Healthcare/Cytiva, 17-0756-01) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... The elutate was further gel filtered over a Hi-Load 16/60 Superdex-200 pg column (GE Life Sciences, Cytiva) in buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the lysate was ultracentrifuged at 22 000 g for 35 min at 4°C and the supernatant was filtered on 0.22 mm filters and then loaded on a His-Trap 5mL column (GE Healthcare) equilibrated with 50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Developmental Biology 2021Quote: ... Chemiluminescence was detected by ECL Prime Western Blotting Detection Reagents (RPN2232, GE Healthcare, Chicago, IL, USA) using the Image Quant LAS 4000 mini (GE Healthcare) ...
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bioRxiv - Developmental Biology 2021Quote: ... Chemiluminescence was detected by ECL Prime Western Blotting Detection Reagents (RPN2232, GE Healthcare, Chicago, IL, USA) using the Image Quant LAS 4000 mini (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... the cell pellets were resuspended in 10 mL of 40% Percoll gradient (GE Healthcare, Chicago, IL) for centrifugation at 700 g for 20 min at room temperature without brake ...
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bioRxiv - Molecular Biology 2020Quote: ... Mononuclear cells were enriched using a Ficoll gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, IL) for 25min at 2,000g at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... The SpaO/SpaOC/OrgB complex was immobilized on HisTrap HP columns (GE Healthcare, Chicago, IL, USA), washed with buffer B3 containing 3 mM ATP and 10 mM MgCl2 and eluted with buffer B3 containing 400 mM imidazole ...
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bioRxiv - Cancer Biology 2019Quote: ... Magnetic resonance imaging (MRI) was conducted by using Signa HDxt 3.0T (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2019Quote: ... 2% Dimethyldichlorosilane in octamethylcyclooctasilane (PlusOne Repel-Silane ES) was purchased by GE Healthcare (Chicago, IL, US). Optical adhesive Norland 65 was purchased from Norland Products ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared supernatant was loaded onto two HisTrap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer A ...
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bioRxiv - Immunology 2021Quote: ... The homogenate was fractioned on a discontinuous 30-70 % Percoll gradient (GE Healthcare, Chicago, IL, USA). Immune cells were collected from the 30/70 % Percoll interphase ...
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bioRxiv - Immunology 2021Quote: ... 25mL of the dilution was overlaid on 15mL of Ficoll-Paque (GE Healthcare, Chicago, IL, USA) and centrifuged at 1200xg for 20 minutes at RT in a swinging-bucket rotor without brake ...
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bioRxiv - Biophysics 2021Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... The Enhanced chemiluminescence (ECL) solutions for the Western blotting system were from GE Healthcare (Chicago, IL). The protease and phosphatase inhibitor cocktails were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2020Quote: ... The supernatant from pDNA transfected cells was purified using Protein G GraviTrap (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and then injected onto a Superdex 200 10/300 FPLC column (GE Healthcare, Chicago, IL, USA) equilibrated with buffer K-phosphate 0,1M pH 7,5 and eluted with a flow rate of 0,9 mL/min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA quality and quantity were assessed by spectrometry in a Nanovue system (GE Healthcare, Chicago, IL). First strand cDNA was produced using 1 μg of RNA ...
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bioRxiv - Immunology 2022Quote: The peripheral blood mononuclear cells (PBMC) were extracted using Ficoll-Paque (GE Healthcare, Chicago, IL, USA) gradient centrifugation ...
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bioRxiv - Genomics 2019Quote: ... and developed using GE Healthcare AmershamTM ECLTM Western Blotting Detection Reagents (6883S, GE Healthcare, Chicago, IL). Image Lab Software (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... The signals were visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA). Image data were acquired with an ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Bioengineering 2019Quote: ... 21th and 42th days using a digital GE model E7843X system (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Pathology 2020Quote: ... Immunoreactive bands were detected using Amersham ECL prime immunoblotting detection reagents (GE Healthcare, Chicago, IL, USA) on an Azure c600 Chemiluminescent imager (Azure Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were then sealed and imaged on the IN Cell 2500 HS (GE Healthcare, Chicago, IL) automated high-content imaging system ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were blotted on a 0.45 μM pore size nitrocellulose membrane (GE Healthcare, Chicago, IL, USA) using a Transblot SD semi-dry transfer system (Bio-rad ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from peripheral blood using a commercial column-based solution (GE Healthcare, Chicago, IL). DNA quantifications were performed in a Qubit dsDNA HS Assay (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Buffer exchange was performed by running extracts through Sephadex resin columns (GE Healthcare, Chicago, IL, USA) and eluting with HEPES buffer (pH 7.4) ...