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Citations for GE Life Sciences :
901 - 950 of 1803 citations for IL 1R1 CD121a Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Protein purification was either carried out by gravity flow Ni NTA Superflow columns (Iba) or FPLC using a His-TrapFF 1ml column (GE Healthcare, Munich, Germany). Concentrated protein eluates were stored at 4°C.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... the MvaT 1-62-His and MvaT 79-124-His were loaded on a HiTrap Q HP 5ml and HiTrap SP HP 5ml column (GE healthcare Life sciences) respectively and eluted by applying a NaCl gradient (from 0.1 to 1 M) ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Biochemistry 2019Quote: ... adjusted to 0.15 M NaCl and 1 % (v/v) Triton X-100 and were applied to a 1 mL Hi-Trap heparin column (GE Healthcare Life Sciences) equilibrated with a modified phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
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bioRxiv - Synthetic Biology 2019Quote: ... Clarified supernatants were then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was then collected for purification using a 5 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). The column was first equilibrated using five volumes of His-tag Buffer + 10 mM imidazole and the supernatant was loaded into de column ...
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bioRxiv - Genetics 2019Quote: ... The final antisera were purified separately using His-DBN-1(1-132) bound to NHS-activated Sepharose High Performance (GE Healthcare Life Science). Both purified antibodies were tested in western blot ...
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bioRxiv - Synthetic Biology 2024Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5 ml column, GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Synthetic Biology 2024Quote: ... Clarified supernatant containing LbuCas13a was purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Developmental Biology 2019Quote: ... normal mouse IgG (GE Healthcare)
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG (GE Healthcare) or anti-rat IgG (Thermo Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... anti-mouse IgG (GE Healthcare, distributed by VWR in Ismaning ...
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bioRxiv - Developmental Biology 2020Quote: ... or anti-mouse (GE Healthcare) antibodies linked with horseradish peroxidase (HRP ...
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bioRxiv - Biochemistry 2021Quote: ... The reducing agent was removed by passing twice through a NAP-5 desalting column (GE Healthcare, Chicago, IL) equilibrated with G-buffer lacking β-ME ...
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bioRxiv - Microbiology 2020Quote: ... Gels were washed in distilled water and imaged using an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA).
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bioRxiv - Developmental Biology 2020Quote: ... The membrane was washed again prior to exposure to ECL Western blot detection reagent (GE Healthcare, Chicago, IL) and GeneMate Blue Autoradiography Film (VWR ...
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bioRxiv - Genomics 2020Quote: ... PBMCs were isolated from blood samples by Ficoll-Paque™ PLUS density gradient centrifugation (GE Healthcare, Chicago, IL) and frozen in fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2019Quote: ... The eluted protein was concentrated and loaded on a Superdex 200 16/60 column (GE Healthcare, Chicago, IL) pre-equilibrated with PBS ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
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bioRxiv - Molecular Biology 2019Quote: ... we performed Western blotting per the protocol provided with the ECL (Enhanced Chemiluminescence) Detection System (GE Healthcare, IL). Furthermore ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
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bioRxiv - Biochemistry 2020Quote: ... were subjected to the short column with 2 ml Sephacryl S-500 resin (GE Healthcare Life Sciences, IL) equilibrated with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Analyses were performed using Amersham ECL Prime Western Blotting Detection Reagent (RPN2232) (GE Healthcare Life Sciences, Chicago, IL), and then the membranes were scanned using Image Quant LAS4010 (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were isolated from whole blood by density-gradient centrifugation using Ficoll-Paque™ (GE Healthcare, Chicago, IL). Total cellular DNA was extracted and used in subsequent PCR experiments.
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The specific band was detected using the ECL Prime Western Blotting Detection Reagent (GE Healthcare, Chicago, IL, USA) with the ImageQuant LAS 4000 digital imaging system (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were purified from the remaining cells by overlaying on a Ficoll density gradient (GE Healthcare, Chicago, IL) following centrifugation in Ficoll-Paque Centrifugation Media (GE Healthcare ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... The homogenate was clarified by centrifugation and mixed with Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL). After extensive washing ...
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bioRxiv - Biochemistry 2020Quote: ... Sera-Mag Oligo(dT)-coated magnetic particles and Hybond-N+ membranes were obtained from GE Healthcare (Chicago, IL). GENEzol™ reagent was purchased from Geneaid Biotech (New Taipei ...
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bioRxiv - Immunology 2022Quote: ... the complex was purified by size exclusion chromatography (Superdex 200 Increase size exclusion column, GE Healthcare, Chicago, IL) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2019Quote: ... and 0.1 mM EDTA] was further purified by using Hitrap Q HP column (GE Healthcare, Chicago, IL, USA) and AKTA start (GE Healthcare) ...
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bioRxiv - Zoology 2020Quote: ... for 1 h at room temperature and developed in ECL (Amersham ECL Select; GE Healthcare, Chicago, IL, USA). Bands were visualized with the MultiImage III FluorChem Q (Alpha Innotech ...
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bioRxiv - Biochemistry 2020Quote: ... This protein was applied to a 16/600 Superdex 75 preparative size exclusion column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A ...
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bioRxiv - Molecular Biology 2020Quote: ... The NPC suspension was subjected to a density gradient centrifugation using Percoll (Lot 10221921, GE Healthcare, Chicago, IL) at 900 xg for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
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bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
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bioRxiv - Molecular Biology 2019Quote: Imaging was performed on the high-resolution GE Discovery 690 ADC PET/CT System (GE Healthcare, Chicago, IL) at 6 ...
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bioRxiv - Genetics 2019Quote: ... Antibody signals were developed using ECL Western Blotting Detection Reagent (GE Healthcare, IL, USA Catalog number: 95038-560) and bands were quantified with Gel Doc system (Bio Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare, Chicago, IL, USA) using 25 mM NaPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... the elution was desalted into 50 mM ABC buffer using HiPrep Desalting column (GE Healthcare: Chicago, IL, USA) and lyophilized into 100 nmol per aliquot.
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... Hybridising bands were visualized using the ECL™ Select western blotting detection reagent (GE Healthcare, Chicago, IL, USA) in a LAS-3000 detection system (Fujifilm España ...