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Citations for GE Life Sciences :
701 - 750 of 3680 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with either Glutathione Sepharose 4B (GE Healthcare, Chicago, IL, USA), Complete His-Tag Purification Resin (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare) for 2 h at 4°C with gentle shaking to bind GST-4×Ub ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads.
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare) for 2 h at 4°C with gentle shaking to bind GST-TEV-NAP1 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare) for 2 h at 4°C with gentle shaking to bind GST-NDP52 ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant was incubated with glutathione-sepharose (0.5 ml per liter culture; GE Healthcare 17513201) for 3 h before elution with lysis buffer + 50 mM glutathione (Applichem A2084,0025) ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences; Table S4).
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bioRxiv - Microbiology 2024Quote: ... The glutathione beads were finally treated with benzamidine-coated beads (GE Healthcare Life Science, 17512301) to remove the factor Xa and dialyzed for the final purification in a 75 mM KAc ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Biophysics 2021Quote: ... GST eluates were further purified with an anion exchange column (HiTrap Q HP, GE Healthcare) and the peak fractions were pooled for co-immunoprecipitation experiments ...
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bioRxiv - Plant Biology 2020Quote: ... The bound HY5 was detected by anti-GST-HRP conjugate (RPN1236; GE Healthcare Bio-Sciences). Membranes were developed and visualized as described above ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved GST was separated by anion exchange chromatography (HiTrap Q HP 5 mL (GE Healthcare)) ...
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bioRxiv - Biochemistry 2022Quote: ... followed by GST column purification using the ÄKTA start chromatography system (GE Healthcare, Chicago, IL). Cell lysate supernatant was added to the column containing nickel resin and it was recirculated across the column by a peristaltic pump overnight at 0.5 ml/min ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion V5-Ydj1 was subjected to site-specific cleavage with PreScission Protease (GE Healthcare) to remove the GST tag ...
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bioRxiv - Microbiology 2019Quote: ... Purifications of the GST-tagged recombinants were performed as described by the manufacturer (GE Healthcare). For expression in mammalian cells ...
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bioRxiv - Cell Biology 2020Quote: ... while Dcp1a was eluted by excision of the GST moiety trough PreScission protease (GE Healthcare) incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was cleaved from the GST tag by incubation with PreScission protease (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 and affinity-purified with GST beads (#17-0756-01, GE Healthcare Life Sciences). 293T cells overexpressing indicated proteins were treated with DNA damage reagents and lysed ...
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bioRxiv - Biochemistry 2020Quote: ... and cation exchange (Source S, GE healthcare) chromatography followed by gel filtration (Superdex200 ...
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bioRxiv - Molecular Biology 2023Quote: ... Series S SA sensor chips (GE Healthcare) were used in all experiments ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs of full-length TPR-CHAT were synthesized using the bacterial expression vector pGEX-6P-1 (GE Healthcare, with an N-terminal GST tag). Point mutations were introduced using Fast Site-Directed Mutagenesis Kit in the Sb-gRAMP gene and verified by DNA sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was incubated with 10 ml of Glutathione Sepharose 4B resin from GE Healthcare, equilibrated with the same buffer for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was first purified by affinity chromatography using Glutathione Sepharose 4B resin (GE Healthcare) and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The soluble fraction was first purified by affinity chromatography using Glutathione Sepharose 4B resin (GE Healthcare) and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... the purified elution from Talon® resin was further passed through the glutathione sepharose (GE healthcare). Removal of the GST-tag from human and zebrafish Vps29 was performed using on-column cleavage with thrombin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatants were loaded on the columns filled with 0.25 mL of Glutathione Sepharose 4B (GE healthcare) at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The cleared cell lysates were incubated overnight with glutathione-Sepharose 4B beads (GE Healthcare Life Sciences) in the presence of 5 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants from each sample were then incubated with glutathione-sepharose beads (GE Healthcare Life Sciences) to immunoprecipitate the GST tagged proteins ...
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bioRxiv - Immunology 2023Quote: ... Protein purification was performed using glutathione sepharose 4B resin according to the manufacturer’s instructions (GE Healthcare). Eluted proteins were then dialyzed and concentrated using an Amicon Ultra-15 10 KDa cutoff (Millipore).
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bioRxiv - Molecular Biology 2023Quote: ... The input was collected from the mix and 50μl of equilibrated glutathione sepharose 4B (GE healthcare) were added to the reaction mix ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
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bioRxiv - Biochemistry 2023Quote: ... the cleared lysate was incubated with 500 µL glutathione agarose slurry (GE Healthcare, #17-0756-01) (resuspended 1:1 in lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1000 μg of cell lysates were incubated overnight with Glutathione Sepharose™ beads (GE Healthcare, UK) at 4 °C on a rotator ...
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bioRxiv - Biochemistry 2024Quote: ... the soluble fraction was incubated in batch for 2 h with glutathione Sepharose 4B (GE Healthcare), previously equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... HCoV-HKU1 S-2P and HCoV-OC43 S-2P were purified over a Superose6 Increase column (GE Life Sciences). HCoV-NL63 and HCoV-229E alpha coronavirus spike proteins were purchased from Sino Biological ...
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bioRxiv - Immunology 2021Quote: ... HCoV-HKU1 S-2P and HCoV-OC43 S-2P were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Immunology 2020Quote: ... HCoV-HKU1 S-2P and HCoV-OC43 S-2P were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Neuroscience 2021Quote: ... Supernatant was filtered with a 0.45 µm filter then left to equilibrate overnight with 1 mL of Glutathione Sepharose 4B affinity resin (GE Healthcare, Pittsburgh, PA, USA, #17-0756-01). Glutathione Sepharose 4B resin and bound protein was applied to a 10 mL disposable plastic column and washed thrice with 3 mL of wash buffer (GST Buffer + 0.1% Triton X-100) ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescent signals were developed using Typhoon (GE Healthcare). The intensity of the bands was quantified by densitometry using ImageQuant software.
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bioRxiv - Biochemistry 2019Quote: ... The synthesized ltgA deletion gene was cloned into a GST-fusion pGEX-4T1 (GE Life Sciences) plasmid as described above ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was cleared by ultracentrifugation and supernatant was incubated with GST-4B beads (GE Life Sciences) for 1H ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... GST-SENP was captured and removed from the eluate by incubation with GSH beads (GE Healthcare). Purified PLpro was further concentrated using an Amicon 5 kDa spin filter to a final concentration of 1 mg/ml and stored at −80 °C (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: TFEB fragments were cloned in frame with GST in pGEX-2T (GE Healthcare, 27-1542-01). Overnight bacterial culture (E ...
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bioRxiv - Molecular Biology 2021Quote: ... the cleared supernatant was collected and incubated with 100 μl of GST Sepharose beads (GE Healthcare) for 1-2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-A3C fusion enzyme containing slurry was treated with 0.02 units/μl thrombin (GE Healthcare) for a minimum of 4 hours at room temperature to release A3C from the GST tag ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was released from the GST beads by thrombin cleavage and purified by MonoQ (GE Healthcare) ion-exchange chromatography ...