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Citations for GE Life Sciences :
551 - 600 of 3680 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
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bioRxiv - Biophysics 2022Quote: ... A Resource S (GE Healthcare) cation exchange column was equilibrated with resuspension buffer with 1% n-octyl-β-D-glucoside and the sample was injected and washed with the same buffer ...
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bioRxiv - Cell Biology 2020Quote: ... backside blotted for 5-10 s with Whatman filter paper grade 1 (GE Healthcare) and vitrified in liquid ethane using a home-made manual plunger.
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bioRxiv - Molecular Biology 2021Quote: ... The Flow-Through was loaded in a 1 ml RESOURCE S column (GE Healthcare), and eluted through a 25 ml gradient (100 mM-1 M NaCl) ...
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bioRxiv - Biochemistry 2020Quote: ... The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare) at 95% humidity ...
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bioRxiv - Immunology 2022Quote: ... HCoV-OC43 S ectodomain was purified using a 1-ml StrepTrap column (GE Healthcare). All purified proteins were then concentrated using a 100 kDa centrifugal filter (Amicon Ultra 0.5 mL centrifugal filters ...
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bioRxiv - Cell Biology 2019Quote: ... After rotation with Glutathione Sepharose 4B beads (GE Healthcare, 0.5 mL bed volume) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was mixed with 50% slurry of glutathione sepharose 4B (GE healthcare) with gentle agitation at RT for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto 5 mL of glutathione sepharose 4B (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... the supernatant was bound to a glutathione affinity column (GSTrap FF, GE Healthcare) in buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2019Quote: ... strain BL21 and purified using Glutathione-Sepharose 4B beads (71024800-GE, GE Healthcare). GST-Myc-CDK4 ...
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bioRxiv - Cell Biology 2021Quote: ... and the resulting supernatant was incubated with Glutathione Sepharose 4B beads (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21(DE3) pLysS cells and purified using glutathione agarose beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of Glutathione Sepharose 4 Fast Flow (GE Healthcare, #GE17-5132-01) were packed onto Poly-Prep chromatography columns (Biorad ...
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bioRxiv - Cell Biology 2022Quote: ... and added to 0.625 mL of glutathione-sepharose beads (GE Healthcare, GE17-0756), which were pre- equilibrated in equilibration buffer (1X PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was then incubated with 20 μL Glutathione Sepharose 4B (GE Healthcare), Amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by addition of 10 µl glutathione-agarose beads (GE Healthcare) and further incubation for 1 hr with rotation at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysates were then incubated with Glutathione Sepharose 4 Fast Flow (GE Healthcare) to immobilize GST-fusion proteins.
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were incubated with 30 μl prewashed Glutathione sepharose 4B slurry (GE Healthcare) per 1 ml lysate ...
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bioRxiv - Cancer Biology 2019Quote: ... for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for GST-tagged proteins at 4 °C following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences) for the GST-tagged proteins ...
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bioRxiv - Immunology 2019Quote: ... Clarified lysates were incubated with Glutathione Sepharose 4B resin (GE Healthcare Life Sciences) at a ratio of 1:40 ...
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bioRxiv - Molecular Biology 2020Quote: ... HDM2 containing supernatant was loaded onto a Glutathione Sepharose 4B resin (GE Healthcare) column that had been already equilibrated with GST binding buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... The samples were then incubated with Glutathione-Sepharose 4B (GE Healthcare Life sciences) for 1 h at 4°C and the beads were washed with lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... and then purified on glutathione-Sepharose4B according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... and the soluble lysate was purified over a glutathione affinity column (GE Healthcare). Bound complex was eluted via overnight on-column TEV cleavage to remove the GST-and His-tags ...
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bioRxiv - Biochemistry 2021Quote: ... the expressed proteins were first purified by Glutathione Sepharose 4B beads (GE Healthcare), and then further purified with a HiLoad 16/600 Superdex 200 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... A 20,000 × g supernatant was incubated with glutathione Sepharose 4B resin (GE Healthcare) for 3 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with pre-equilibrated glutathione sepharose 4B (GE Healthcare) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was then incubated with 20 μL Glutathione Sepharose 4B (GE Healthcare) or Amylose resin (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a glutathione-Sepharose-4B affinity column (GE Healthcare) equilibrated with lysis buffer and washed until a stable baseline was obtained ...
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bioRxiv - Plant Biology 2023Quote: ... then 10 μL of Glutathione Sepharose 4B beads (17-0756-01, GE Healthcare) was added to the mixture sample and rotated at 4°C for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The PreScission protease was removed by incubation with Glutathione Sepharose beads (GE Healthcare) at 4 °C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2 mM MgCl2) with Glutathione Sepharose™ 4B (GE healthcare 17-0756-01) or Amylose Resin (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was added to 200 μl Glutathione-Sepharose 4B-beads (GE Healthcare) and incubated for 2 h at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysate supernatant was loaded on a Glutathione Sepharose 4B column (GE Healthcare). The column was washed with TBS (20 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from the lysate using Glutathione-sepharose 4B (GE Healthcare). His-tagged protein expression was induced by shifting to 15°C and adding 0.1 mM IPTG ...
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bioRxiv - Plant Biology 2023Quote: ... After the proteins were purified by Glutathione Sepharose 4 Fast Flow (GE Healthcare) and Amylose Resin (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... coli Rosetta/DE3 and the proteins purified using glutathione resin (GE Healthcare, USA) as described (Fliegmann et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... which was then incubated with Glutathione Sepharose 4B beads (Cytiva – formerly GE Healthcare) (5ml per L culture ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with glutathione (GSH) beads (GE Healthcare, #17-0756-01) and incubated for 20 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Microbiology 2020Quote: ... 1/10th of Cy5-CTP (Amersham CyDye Fluorescent Nucleotides Cy5-CTP, GE Healthcare Life sciences) was included in the IVT reaction ...
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bioRxiv - Cell Biology 2020Quote: ... freshly purified GST-RILP protein bound to GSH-Sepharose 4B beads (GE Healthcare) was incubated with extracts from transfected HEK293T cells (one 10-cm diameter dish per assay ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... GST tags were cleaved with PreScission protease per the manufacturer’s instructions (GE Healthcare). Isolated CFP1 fragments were dialyzed in 25% glycerol ...