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Citations for GE Life Sciences :
651 - 700 of 1424 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The enriched DNA was spotted onto an Amersham Hybond-N+ membrane (GE Healthcare, Cat# RPN203B), UV cross- linked for 10 min and incubated with streptavidin-HRP (Beyotime Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... RNA (2 μg) of each group was spotted on a Hybond-N+ membrane (GE Healthcare), followed by UV cross-linking (120 mJ/cm2 ...
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bioRxiv - Biophysics 2020Quote: ... Kif14 constructs were finally separated from the MBP tag by size-exclusion chromatography on a superpose 6 10/300 (GE Healthcare) column equilibrated with BRB80 buffer (80 mM Pipes (pH 6.8) ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare). The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were isolated from the medium exploiting the affinity of the 6xHis tag for the HisTrap Excel (GE Healthcare, USA) affinity column ...
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bioRxiv - Biochemistry 2020Quote: ... The 8 × His tag was removed by TEV protease and tag-free WDR522-334 was further purified by gel filtration using a Superdex 75 (10/300) increase column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... It was first purified by a glutathione-affinity step and then subsequent to tag removal following incubation with 3C protease further purified on a MonoS ion-exchange (GE Healthcare) column equilibrated into 25 mM MES pH 6.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Cell Biology 2021Quote: Full-length Pak1 constructs (wild type and catalytically inactive) and full-length Sts5 constructs (wild type and phosphomutant) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare) for expression in BL21 DE3 Escherichia coli ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by the addition of PreScission Protease (~100 μL at 76 mg/mL) (GE Healthcare) and the eluted ATAD2B bromodomain protein was concentrated to a total volume of approximately 3 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2021Quote: ... The polyhistidine tag was removed by overnight incubation with TEV protease and further purified by Superdex-75 size-exclusion column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Further polishing of the complex prior SEC and after tag cleavage with PreScission protease was carried out by loading onto a cation-exchange (CE) Resource-S column (GE Healthcare) equilibrated in 10mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was passed through the HisPur resin to remove eGFP-His8 tag and TEV protease and further purified using Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) in SEC buffer containing 0.06% UDM /0.2% DM ...
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bioRxiv - Immunology 2022Quote: ... the proteins were treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by a combination of chromatography using HiTrap Heparin (GE Healthcare), Hitrip SP (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... the proteins were treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by size-exclusion chromatography on Superdex 75 Increase 10/300 (GE Healthcare) column ...
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...
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bioRxiv - Immunology 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin followed by gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... TEV and residual affinity tag was removed by passing the protein through a Superdex 200 Increase 10/300 GL (GE Healthcare) in SEC buffer (30 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare) in NMR buffer (20 mM sodium phosphate pH 6.5 ...
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bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... The cleaved fused 6His-SUMO-tag was removed from the FLAG-MCP by reloading the sample onto the second HisTrap column (GE Healthcare). The FLAG-MCP was further purified by chromatography using a HiTrap Heparin SP column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Microbiology 2023Quote: ... the negatively charged N-terminal His- and BAP-tag fused MBP protein was separated from HphA by ion-exchange chromatography with HiTrap Q HP 5ml column (GE Healthcare). The flow-through ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Plant Biology 2024Quote: ... the cleaved tag from the 6xHis-MBP-Pwl2 purification was removed from the solution using a MBPTrap HP dextrin sepharose column (GE Healthcare) and subsequently eluted using SEC buffer supplemented with 10mM maltose.
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Biochemistry 2023Quote: ... the His6-NusA-tag was removed using the HRV3C protease and was further purified by SEC on Superdex 75 Increase 10/300 (GE Healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Biochemistry 2023Quote: ... the protein was treated with HRV3C protease to cleave the His6-NusA-tag and were further purified by a series of chromatography purification using HiTrap Heparin (GE Healthcare), Hitrip SP (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Separation of cleavage tags and aggregated proteins was performed using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20□mM citric acid pH 5.0 ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
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bioRxiv - Microbiology 2024Quote: ... The 6ξ-His tag was cleaved for 16 h at 4 °C using 1U of thrombin per 100 µg of total protein (GE Healthcare). The cleaved proteins were then passed through a Ni-NTA column to remove the cleaved 6ξ-His tag and any uncleaved protein ...
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bioRxiv - Biochemistry 2024Quote: ... after affinity purification and tag removal was performed using a HiLoad Superdex 75 pg 16/60 or Superdex 75 10/300GL column (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... GST-tag removed fraction was further purified by size-exclusion chromatography (SEC) using a Superdex 200 (16/600) column (GE Healthcare) equilibrated with a buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... The resultant cDNA was purified using G50 column (GE healthcare) and subjected to second strand synthesis (NEBNext Second Strand Synthesis Module) ...
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bioRxiv - Genomics 2021Quote: ... The resultant cDNA was purified using G50 column (GE healthcare) and subjected to second strand synthesis (NEBNext Second Strand Synthesis Module) ...
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bioRxiv - Molecular Biology 2024Quote: ... The generated cDNA was purified using G50 column (GE healthcare) and subjected to second strand synthesis (NEBNext Second Strand Synthesis Module) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant cDNA was purified using G50 column (GE healthcare) and subjected to second strand synthesis (NEBNext Second Strand Synthesis Module) ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA generated was further purified using G50 column (GE healthcare) and subjected to second strand synthesis (NEBNext Second Strand Synthesis Module) ...
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bioRxiv - Molecular Biology 2022Quote: ... Hexahistidine-tagged proteins in the soluble fraction were affinity purified using a nickel-charged His-trap immobilized metal affinity chromatography (IMAC) column (GE Healthcare, UK). Washing was carried out with lysis buffer and recombinant proteins were eluted with the same buffer containing 500 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... histones were further purified by tandem ion exchange chromatography using HiTrap Q and Hi Trap SP anion and cation exchange columns (GE Life Sciences). Histone-containing fractions were pooled ...
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bioRxiv - Immunology 2020Quote: ... Protein containing cell culture supernatants were harvested and clarified media was purified by Immobilized Metal Affinity Chromatography (IMAC) using His Trap™ Excel (GE Healthcare) using PBS buffer plus 15mM Imidazole ...