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Citations for GE Life Sciences :
451 - 500 of 1424 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare) in 20 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The tag cleaved Ub was further purified by size exclusion chromatography through a Superdex 75 column (GE Healthcare) in buffer A.
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Plant Biology 2024Quote: ... The tag-free CMT2 proteins were further purified through ion-exchange chromatography on a Heparin column (GE Healthcare) and size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Biochemistry 2020Quote: ... The concentrated sample was further purified over a Superdex 200 Hi-load 16/60 gel filtration column (GE Healthcare) pre-equilibrated with 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: The interaction between PttP1 and PttP2 was studied by a pulldown assay using His SpinTrap™ columns (GE Healthcare) with a bed volume of 200 μl ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... and loaded to a Hi-Load 16/600 Superdex-200 pg (preparation grade) size-exclusion chromatography column (GE Healthcare) equilibrated with the gel filtration buffer (100 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... most of the proteins were loaded onto a Hi-trap Q HP anion exchange column (GE Healthcare, cat. 17115401). The final purification step involved using a size exclusion column ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant containing the solubilised membrane proteins was pooled and applied to a His-TRAP nickel affinity column (GE Healthcare) using an ÄKTA Protein Purification System (Cytiva) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs were then transferred over night to Amersham Hybond N+ membranes (GE Healthcare) by capillarity with 10X SSC transfer buffer ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then electroblotted onto Amersham Hybond-N+ nylon membrane (GE Healthcare, USA) for 90 min at 35V in 0.5x TBE using the TransBlot Mini transfer apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was transferred overnight to a Hybond N+ nylon membrane (GE Healthcare) by upward capillary transfer ...
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bioRxiv - Genomics 2021Quote: ... After capillary transfer in 10× SSC onto a Hybond N+ membrane (GE Healthcare), RNA was UV-crosslinked and stained with methylene blue to visualise ribosomal RNA bands ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then electroblotted onto Amersham Hybond-N+ nylon membrane (GE Healthcare, USA) for 90 min at 35V in 0.5x TBE using the TransBlot Mini transfer module (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... and transferred onto Hybond-N+ charged nylon membranes (GE Healthcare, Chicago, Illinois, USA) by capillary transfer in 20×SSC ...
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bioRxiv - Molecular Biology 2022Quote: ... separated by electrophoresis and transferred to a nylon membrane Hybond N+ (GE Healthcare). The probes for the upstream regions of the pks1 (oligonucleotides 9 and 10 ...
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bioRxiv - Immunology 2020Quote: ... N-hydroxysuccinimide (NHS) and ethanolamine (H2N(CH2)2OH) were purchased from GE Healthcare. HpARI ...
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bioRxiv - Molecular Biology 2020Quote: ... 5.55 % formaldehyde and transferred onto a nylon membrane (Amersham’s Hybond N+, GE Healthcare). After transfer ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was transferred to a positively charged nylon membrane (Hybond N+, GE Healthcare) through semi-dry electrophoretic transfer cell (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA samples were loaded onto an Amersham Hybond-N+ membrane (RPN119B, GE Healthcare) using a Bio-Dot apparatus (#170-6545 ...
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bioRxiv - Genetics 2022Quote: ... Digested DNA was transferred by capillarity to a Hybond-N membrane (GE Healthcare) and hybridized with 32P-labeled probe which is specific to CaLCuV DNAA ...
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bioRxiv - Molecular Biology 2022Quote: ... the agarose gel was transferred to a Hybond-N+ nylon membrane (GE Healthcare). An 819-bp fragment containing the full length sZF cDNA was labeled by random priming with [α-32P] dCTP and used as a probe.
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bioRxiv - Molecular Biology 2022Quote: ... 300 V and transferred onto a Hybond-N+ membrane (GE Healthcare Life Sciences) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2022Quote: ... followed by semi-dry transferring onto Hybond-N+ hybridization membranes (GE Healthcare, RPN303B). The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer) ...
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bioRxiv - Molecular Biology 2024Quote: ... the gel was photographed and RNA was transferred to Hybond-N+ (GE Healthcare) by standard capillary transfer.
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... Electroblotting of the RNA onto a Hybond-N+ membrane (GE Healthcare Life Sciences) was performed at 50 V ...
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bioRxiv - Plant Biology 2023Quote: ... the RNA was blotted onto the HybondTM-N+ nylon transfer membrane (GE Healthcare) by soaking in 20× SSC (3.0 M NaCl and 0.3 M sodium citrate ...
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bioRxiv - Immunology 2024Quote: ... An N-hydroxysuccinimide ester was formed on a CM5 sensor chip (GE Healthcare) surface according to a procedure recommended by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... The N protein was then run over a HisTrap FF column (GE Healthcare) in Buffer A (20 mM Tris pH 7 ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA was transferred overnight to a Hybond-N+ nylon membrane (GE Healthcare) by upward capillary transfer with 20x SSC buffer ...
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bioRxiv - Microbiology 2024Quote: ... RNAs were then transferred on Hybond N+ nitrocellulose membrane (GE Healthcare Life Sciences). Membranes were hybridized with specific digoxygenin (DIG)-labelled probes targeting RNA of interest or 5S rRNA (loading control) ...
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bioRxiv - Neuroscience 2024Quote: ... The extracted mRNA was denatured and spotted onto Hybond N+ membrane (GE Healthcare), then cross-linked using a Stratalinker UV Crosslinker 2400 (Stratagene) ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 μg of total RNA was used to synthesize the first-strand cDNA using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (5 μg) was used to synthesize the first-strand complementary DNA (cDNA) using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer following the manufacturer’s indications ...
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bioRxiv - Cell Biology 2021Quote: Tag-free syndapin I and III were generated by expressing both proteins in the pGEX-6P vector (GE Healthcare) and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...