Labshake search
Citations for GE Life Sciences :
751 - 800 of 1424 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... RNA was transferred onto Hybond N+ positive charged nylon membranes (Cat# RPN303B; GE Healthcare, Little Chalfont, UK) using semi-dry transfer and cross-linked using ultraviolet light ...
-
bioRxiv - Genomics 2021Quote: ... Gels were rinsed in 0.5x TBE and RNA was transferred onto a Hybond N+ membrane (GE Healthcare) in 0.5x TBE by semi-dry transfer (Bio-Rad Transblot ...
-
bioRxiv - Biochemistry 2024Quote: ... consisting of a Protran nitrocellulose membrane (Whatman) followed by a Hybond N+ nylon membrane (GE Healthcare Bioscience). Subsequently ...
-
bioRxiv - Genetics 2023Quote: ... The DNA fragments were UV-nicked prior to transfer to Hybond N + Nylon membrane (GE Healthcare, RPN203B) as described in the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... separated on 1.2 or 1.8% formaldehyde-MOPS agarose gels prior transfer onto Hybond-N+ membranes (GE Healthcare) overnight ...
-
bioRxiv - Genetics 2023Quote: ... The RNA was transferred overnight by capillarity to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) and covalently bound to the membrane using a Stratalinker UV crosslinker ...
-
bioRxiv - Genetics 2022Quote: ... The RNA was transferred overnight by capillarity to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) and covalently bound to the membrane using a Stratalinker UV crosslinker ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 μL of total RNA was dropped onto an Amersham Hybond-N+ membrane (GE Healthcare, cat# RPN203B), then the membrane was briefly air dried and crosslinked using Stratalinker 2400 UV Crosslinker twice using Autocrosslink mode ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was resolved through denaturing polyacrylamide/urea gels and transferred to Hybond N+ membranes (GE Healthcare, UK) using standard protocols ...
-
bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
-
bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
-
bioRxiv - Molecular Biology 2023Quote: ... Eluate from the His purification was then further purified by size-exclusion chromatography using a Superdex 200 HiLoad 16/600 column (GE Healthcare: 28-9893-35) in CSF-XB buffer ...
-
bioRxiv - Plant Biology 2022Quote: ... lysed and the protein purified by nickel affinity chromatography using the His- Trap HP column coupled with size-exclusion chromatography (SEC) using the HiPrep 26/60 Sephacryl S-200 column (GE Healthcare Life Sciences, UK). The protein was eluted into a buffer containing 30 mM Tris ...
-
bioRxiv - Plant Biology 2024Quote: ... The fusion protein His-SUMO-ApcI was purified by loading the cell lysate supernatant to a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
-
bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were purified via a two-step chromatography process involving nickel-affinity chromatography via the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S200 10/300 GL column ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
-
bioRxiv - Neuroscience 2023Quote: ... The remaining tag and protease were removed by reverse Ni-NTA purification in batch mode (Ni-Sepharose High Performance; GE Healthcare, 17-5268-02). Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare ...
-
bioRxiv - Genomics 2020Quote: ... cDNA was exchanged into water using G-50 columns (GE Life Sciences) the volume increased to 68 μl using nuclease free water ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reaction products were dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) followed by hybridized at 50 °C with end-labeled (CCCTAA)4 to detect C-circles or (TTAGGG)4 to detect G-circles.
-
bioRxiv - Molecular Biology 2021Quote: ... The eluted DNA was dot-blotted and UV cross-linked onto a Hybond N nylon membrane (GE Healthcare) and hybridized with a telomere (or a tubulin ...
-
bioRxiv - Immunology 2021Quote: ... each ectodomain variant was chemically cross-linked to N-hydroxysuccinimide (NHS)-activated Sepharose beads (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... and UNC- 16(1-506) was covalently coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare).
-
bioRxiv - Molecular Biology 2022Quote: RNA was separated on a 16% polyacrylamide gel and transferred to the Amersham Hybond-N+ membrane (GE Healthcare). Probes tRNA-Glu-TTC ...
-
bioRxiv - Plant Biology 2021Quote: ... The RNA gel was electroblotted onto an Amersham Hybond-N neutral membrane (GE Healthcare UK Limited, Buckinghamshire, UK) at 180 mA for 1 h ...
-
bioRxiv - Biochemistry 2020Quote: ... Sera-Mag Oligo(dT)-coated magnetic particles and Hybond-N+ membranes were obtained from GE Healthcare (Chicago, IL). GENEzol™ reagent was purchased from Geneaid Biotech (New Taipei ...
-
bioRxiv - Immunology 2021Quote: ... mRNA was mixed with RNA loading buffer and then carefully spotted onto a Hybond-N+ membrane (GE Healthcare), followed by UV crosslinking ...
-
bioRxiv - Molecular Biology 2023Quote: ... resolved on a 0.8% agarose gel and transferred onto a Hybond-N positively charged membrane (GE Healthcare, UK). Oligonucleotide primers were used to amplify a Maxi-probe (SMD359/6 ...
-
bioRxiv - Microbiology 2022Quote: ... Separated DNA fragments were transferred to the nitrocellulose membranes (Amersham Hybond-N+; GE Healthcare UK Ltd., Buckinghamshire, UK) by capillary transfer ...
-
bioRxiv - Microbiology 2022Quote: ... 0.3 M sodium citrate pH 7.0) and transferred to an Amersham Hybond-N+ membrane (GE Healthcare Life Sciences) by capillary action overnight ...
-
bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
-
bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
-
bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
-
bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare, Little Chalfont, UK) passing the mixture of His-SUMO-ApcI with ULP through the HisTrap column retaining His-SUMO as well as ULP and collecting the flowthrough containing tagless ApcI ...
-
bioRxiv - Cell Biology 2022Quote: ... or anti-mouse (NA931V, GE Healthcare). These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime ...
-
bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution and anti-rabbit secondary antibody (Cell Signaling Technology ...
-
bioRxiv - Immunology 2020Quote: ... anti-mouse IgG-HRP (GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: ... ECLTM anti-mouse IgG (GE Healthcare), and ECLTM anti-rabbit IgG (GE Healthcare ...
-
bioRxiv - Systems Biology 2022Quote: ... or anti-mouse (GE Healthcare NXA9311ML) peroxidase-linked secondary antibody (1:6000 dilution) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and goat-anti-mouse (GE Healthcare) secondary antibodies were used at concentrations of 1:10,000.
-
bioRxiv - Cell Biology 2022Quote: ... HRP-coupled α-mouse (GE Healthcare) 1:10000 ...
-
bioRxiv - Plant Biology 2022Quote: ... sheep anti-mouse (NA931, GE Healthcare) was used at a dilution of 1:50,000 ...
-
bioRxiv - Cell Biology 2024Quote: ... and anti-mouse IgG (GE Healthcare), and reacted with chemiluminescent HRP substrate (Millipore).
-
bioRxiv - Biophysics 2020Quote: ... thrombin cleavage of the His6-tag overnight and final ion exchange chromatography with a MonoQ or MonoS column (Äkta purifying system, GE Healthcare, Little Chalfont, United Kingdom). Synaptobrevin 2 49-96 S79C was labeled with Atto488 maleimide ...
-
bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
-
bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using the First-Strand cDNA Synthesis Kit (GE Healthcare). Quantitative RT–PCR was performed using qPCR MasterMix Plus Low ROX (Eurogentec ...
-
bioRxiv - Biochemistry 2020Quote: ... RT cDNA products were then purified (Illustra G-50 microspin columns, GE Healthcare).
-
bioRxiv - Neuroscience 2020Quote: ... βSyn or γSyn cDNA was inserted into modified pGEX-KG vectors (GE Healthcare), containing an N-terminal TEV protease recognition site ...