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Citations for GE Life Sciences :
6301 - 6350 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The solution was neutralized by adding 1 M HCl and the cDNA was purified with the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). The 5’-3’ junction sequence was amplified by PCR with cox1 specific primers Atcox1-5’(−176..-196 ...
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bioRxiv - Plant Biology 2021Quote: ... 1470 bp) were purified and restricted with BamH1 and EcoR1 prior to subcloning into pGEX-6P-1 (GE Healthcare, Uppsala, Sweden) digested with the same enzymes to produce plasmids to produced N-terminally GST-tagged versions of NoAUREO 2 ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Plant Biology 2021Quote: ... using an AKTA Pure 25 system equipped with HisTrap HP 1 mL column and submitted to gel filtration using a Superdex 200 increase 10/300 GL column (GE healthcare). Purified recombinant proteins were conserved at −80°C in 1x PBS solution containing 10% glycerol.
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bioRxiv - Microbiology 2021Quote: ... The buffer was then adjusted to contain 40 mM imidazole and the rNAs were purified with a HisTrapTM FF 1 ml column (GE Healthcare) using an Akta Start protein purification system (Cytiva) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The sample (100 µL of 1 mg/mL UL21-H6) was injected onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in 20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... and fractionated by linear gradient with end buffer (10 mM Tris-HCl, 1 M NaCl) using an AKTAprime plus (GE Healthcare) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: Yeast Cka1 and Tda1 genes were amplified by PCR from yeast genomic DNA and then cloned into pGEX4T-1 (GE healthcare) vector ...
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer F containing 200 mM imidazole and further purified over a 1 ml ResourceS cation exchange chromatography column (GE Healthcare) using a 20 ml gradient from 100 mM to 500 mM NaCl in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in HRP conjugated secondary antibodies detailed in Table 4 for 1 h at room temperature and developed using Pierce SuperSignal Pico Plus (Pierce) or ECL (GE Healthcare) reagent and imaged on ImageQuant.
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The coding sequences for the N-terminal (63-139 aa) and C-terminal (221-307 aa) domain of OR were cloned to the pGEX-4T-1 vector (GE healthcare) for prokaryotic expression ...
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bioRxiv - Molecular Biology 2022Quote: ... 80 µg of a 5' amino-modified DNA oligonucleotide (Table S1) complementary to target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 ml, GE Healthcare). Binding to the NHS column was performed by recirculation of the small RNA fraction over the complementary NHS columun for 3 hours at 65°C ...
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Cell Biology 2022Quote: Figure 8 Supplement 1 was imaged using a DeltaVision Elite high-resolution imaging system equipped with a sCMOS 2048 × 2048 pixel camera (GE Healthcare). Z-stacks (0.2 μm step ...
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bioRxiv - Cell Biology 2022Quote: ... the codon-optimized sequence was synthesized (BioCat) and inserted into the BamHI and SalI sites of pGEX-6P-1 (GE Healthcare). For expression of ForB-N (aa 1-406 ...
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bioRxiv - Microbiology 2020Quote: ... put immediately on ice and 1 μL spotted on the positively charged nylon membrane (Amersham Hybon™-XL, GE Healthcare, France), which was exposed to UV at 254 nm for 10 min to fix DNA ...
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bioRxiv - Biochemistry 2021Quote: ... Immobilized metal ion affinity chromatography of the His-tagged Pdr5 was performed with a Zn2+ loaded 1 ml HiTrap Chelating column (GE Healthcare) and elution was carried out with a step gradient using low and high histidine buffers (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured according to the ATCC guidelines in 4:1 mixture of Dulbecco’s medium and Medium 1999 supplemented with 10% Hyclone™ FBS (GE Healthcare) and 50μg/ml Gentimicin (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM MgCl2 was added and periplasmic extracts were harvested by centrifugation and the supernatant was loaded onto a 1 ml HisTrap FF column (GE Healthcare). The nanobody was eluted via a linear gradient into 400 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 1 ml overnight bacterial culture using the Illustra bacteria genomicPrep Mini Spin Kit (GE Healthcare, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The mix was allowed to incubate for 1 hour over ice and loaded to a Superdex 200 Increase 10/300 GL size exclusion column (GE healthcare) pre-equilibrated in buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: Fragment screening of MsbA embedded in nanodiscs was performed with the Discovery-ZEN-LIBRARY 1 (Zenobia) in a Biacore 8K instrument (GE Healthcare) using an NTA Series S sensor chip (Cytiva ...
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bioRxiv - Biophysics 2020Quote: ... including an N term extension containing His9-HRV 3C protease site was cloned into the BamHI EcoRI sites in the MCS of pGEX-6P-1 vector (GE Healthcare). Site-directed mutagenesis was performed on the His9-SARS-CoV-2 Nucleocapsid pGEX vector to create the N protein constructs (SI Table XX and sequences were verified using Sanger sequencing ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Cancer Biology 2022Quote: ... Blood was diluted 1:2 with 1× phosphate-buffered saline (PBS) and transferred to a 50 ml falcon tube with Ficoll-Paque PLUS (GE Healthcare) at a 1:2 ratio ...
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bioRxiv - Cell Biology 2022Quote: GST or GST-tagged β’-COP (residues 1-604) or Glo3 constructs (GAP domain, BoCCS region, GRM region) were immobilized on glutathione sepharose resin (GE Healthcare) for one hour on ice ...
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant protein in the soluble cell fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating HP 1-ml column (GE Healthcare) with an ÄKTA purifier FPLC system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Human CFP1 and truncation constructs were subcloned into the pGEX-6P-1 vector encoding an N-terminal glutathione S-transferase (GST) tag (GE Healthcare) prior to expression in Origami cells ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Plant Biology 2019Quote: ... Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA) of biotinylated/streptavidin/horseradish peroxidase complex (GE Healthcare) before a final PBS/BSA rinse ...
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were eluted using a linear gradient (10 column volumes) from 0 to 1 M NaCl in buffer A using an Äkta Purifier (GE Healthcare). Elution fractions (1.6 ml ...
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bioRxiv - Biochemistry 2019Quote: ... 0.1 ml of this solution was subjected to SEC at 293 K on a Superdex 200 10/300 column (GE Healthcare) preequilibrated in 10 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... concentrated using centrifugal filters to bring down the volume to ∼1 mL and injected onto a Superdex 200 16/60 size exclusion column (GE Healthcare) previously equilibrated in HEPES 50 mM ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...