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Citations for GE Life Sciences :
6101 - 6150 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... washed in TBST and detected with anti-Mouse IgG-Horseradish peroxidase (GE Healthcare Life Science, Cat# NA931, 1:5000). For streptavidin staining ...
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bioRxiv - Biochemistry 2023Quote: ... to <1 mL and injected onto a Superdex™ 75 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20mM Tris pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with Cy5-probe ([23]) ...
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bioRxiv - Biochemistry 2023Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted protein was concentrated and incubated with the antibody fragment scFv16 for 2 h on ice at a molar ratio of 1:1.5.48 A Superdex 200 Increase 10/300 column (GE Healthcare) was pre-equilibrated with buffer [20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel was soaked in 1×TBE for 20 min and transferred to a Hybond-N+ membrane (GE Healthcare) for 1 h (15 V ...
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bioRxiv - Biophysics 2023Quote: ... the supernatant was filtered (0.22 μm pore size) and applied to a HisTrap HP 1 mL column (GE Healthcare) for affinity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... PTENα-NTE (residues 1-173) was further purified by ion exchange chromatography (HiTrap SP HP column, GE Healthcare, 17115201) using buffers with 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... TbSmee1(1-400) concentrates were applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare) pre-equilibrated in dialysis buffer ...
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bioRxiv - Genomics 2024Quote: Ten μl of PCR product were then mixed with 1 μl of Streptavidin sepharose™ high performance (GE Healthcare) and 40 μl of PyroMark binding buffer (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant for each nanobody was then applied to one 1 ml Ni-NTA pre-packed column (GE healthcare) pre-equilibrated with base buffer + 30 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... using 10/12% acrylamide/bisacrylamide (29:1) 0.1% SDS gels and transferred to a 0.2 μm PVDF Hybond-P membranes (Amersham/GE healthcare) at 20V for 1h in a semi-dry Trans-Blot SD cell system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 h with HRP-streptavidin in TBS/10% FBS (1:200; GE Healthcare Life Sciences). After a final wash in TBS (3 x 10 min) ...
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bioRxiv - Plant Biology 2020Quote: ... 1 mM EDTA) affinity chromatography followed by size-exclusion chromatography on a HiLoad 16/600 Superdex 200pg column (GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biophysics 2021Quote: ... Cell lysates were centrifuged at 75,000 x g for 25 min and supernatants were loaded on a GSTrap FF 1 mL column (GE Healthcare) using an AKTA Start purification system (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was then supplemented with 5 mM imidazole prior to loading onto a 1-ml HisTrap column (GE Healthcare) attached to an AKTA system at 1 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... GST-PTP1B was purified with a 1 ml GSTrap FF Column on an ÄKTA pure purification system (GE Healthcare GmbH.) using a binary buffer system (buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged to 1xSAB supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... The diluted eluate was then loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, Chalfont St Giles, UK). After loading ...
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bioRxiv - Genomics 2020Quote: ... The membrane was incubated with 1:10,000 dilution of anti-rabbit IgG conjugated to horse radish peroxidase (HRP; GE Healthcare) diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 3.0 (pH neutralization to 7.8 with 1 M Tris-HCl, pH 9.0) using ÄKTA FPLC system (GE Healthcare Life Sciences). The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... were used at 1:10,000 and blots developed with ChemiGlow detection reagent and visualized using ImageQuant LAS 4000 chemiluminescent camera (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime, GE healthcare). Chemiluminescence signals were detected by a LAS 4000 (GE healthcare ...
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bioRxiv - Genomics 2020Quote: ... Mouse Anti-Rabbit IgG (Light Chain Specific) (CST, #93702S) (1:5000) or Rabbit IgG HRP Linked Whole (GE Healthcare, NA9340), respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from donkey) (1:10,000 in 3% skim milk/TBST, NA9340, GE Healthcare) Mouse TrueBlot ULTRA ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from sheep) (1:10,000 in 3% skim milk/TBST, NA9310, GE Healthcare) ECL anti-rabbit IgG ...
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bioRxiv - Cell Biology 2019Quote: ... adjusted to 100 mM Tris-HCl pH 8.0 and 1 mM EDTA and loaded onto a StrepTrap HP column (GE Healthcare) pre-equilibrated in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 1 h at 95,000g and the supernatant was loaded onto a Glutathione Sepharose HP (GE Healthcare) column ...
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bioRxiv - Cell Biology 2019Quote: ... The extract was pre-cleared by centrifugation at 20.000 x g for 45 minutes and the supernatant was incubated for 1 hr with Streptactin Sepharose beads (GE Healthcare) which had been washed 3 times in the lysis buffer ...
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bioRxiv - Biophysics 2019Quote: ... the modified f(Ab)1 fragment was purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05, GE Healthcare Dharmacon) or with siRNAs targeting PDK2 and PYCR1 (Dharmacon ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 35,000g for 20 min and the supernatant from 1 l of cell culture was incubated with 2.5 ml of Glutathione Sepharose beads (GE Healthcare) at 4°C for 6 h The Glutathione Sepharose beads were then collected in a PD10 column and washed with 50 ml of PBS ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was washed in 0.1% SDS/0.1X SSC and hybridized DNA was visualized and quantitated by phosphor-imaging on a Typhoon Scanner (GE Healthcare). The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a 1-ml HisTrap column connected to an ÄKTA Explorer 100 purification system (GE Healthcare), previously equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting eluate was manually applied at room temperature to a 1 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour at 30V onto Amersham Hybond P 0.45 PVDF membranes (GE Life Sciences, Pittsburgh, PA; cat # GE 10600029). After transfer membranes were rinsed in TBST and blocked for one hour in either 5% milk (PERK ...
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed with TBST three times and incubated with sheep anti-mouse or donkey anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (1:4,000, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.1% FA (Fluka) and crosslinked peptides were enriched by separation on a Superdex Peptide PC 3.2/30 column (GE Healthcare), using an Agilent 1100 chromatography system at 95 μL/minute with fraction collection ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was filtered through tandem 1-μm and 0.45-μm filters before Ni2+-affinity purification (HisTrap excel, GE Healthcare). Ni2+-bound proteins were washed with buffer A and eluted directly onto a coupled Strep-Tactin Superflow XT column (IBA ...