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Citations for GE Life Sciences :
6151 - 6200 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... in running buffer (20 mM Tris, pH 8, 150 mM NaCl, 1 mM DTT) supplemented with 0.05% of P20 surfactant (GE Healthcare). Anti-MBP murin monoclonal antibody (Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... and eluted in superdex buffer (20 mM Tris [pH 8.0], 300 mM NaCl, 1 mM TCEP) using the AKTA Explorer FPLC system (GE Healthcare). The protein was concentrated to 7.5 mg/ml.
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bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
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bioRxiv - Microbiology 2020Quote: ... The clarified lysate was then passed using an ÄKTA Purifier FPLC through a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... with a JA30.50 Ti rotor at 20,000 ×g and applied to a HiTrap SP HP column (GE Healthcare; 1 ml) equilibrated in buffer A (20 mM Na-phosphate buffer (pH 7.0) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged at 18500 g for 30 minutes and the supernatant filtered through a 0.22 µm PES filter and loaded onto a 1 mL Ni column (GE healthcare) in buffer L with 10 mM immidazole ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM TCEP) and the sample was loaded onto a 5 mL HiTrap Heparin HP affinity column (GE healthcare) pre-equilibrated with 5% buffer B (50 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of cAMP in lysates from infected THP-1 cells was determined using the cAMP enzyme immunoassay (GE Healthcare) as previously described (57) ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by horseradish peroxidase-conjugated goat anti-rabbit (1:10,000) and detection on Super RX X-ray film (Fujifilm) using ECL Plus reagent (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the resulting supernatant was incubated for 1 hour at 4°C on constant agitation of 1,000 rpm with 100 µL glutathione sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was centrifuged at 18,500 × g for 30 min and the supernatant filtered through a 0.22 µm PES filter and loaded onto a 1 mL Ni column (GE healthcare) in buffer L with 10 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with either 1 ml of Amersham ECL substrate western blotting detection reagent (GE Healthcare lot# 9622301) or for higher sensitivity ...
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bioRxiv - Neuroscience 2021Quote: ... and the supernatant injected (using 1 ml injection loop) in a Superdex 75 10/300GL gel filtration column (GE Healthcare), previously equilibrated with 50mM Tris pH8 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and within 1 h after taking out of the incubator on a commercial structured illumination microscope (DeltaVision|OMXv4.0 BLAZE, GE Healthcare). All the images were acquired using 488 nm laser with power set to either 10% or 31.5% ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed in PBS and incubated overnight in Cy3-conjugated streptavidin (1:1000 dilution; PA43001, GE Healthcare) in “Triton-PBS” (PBS containing 0.3% v/v Triton X-100 [Sigma]) ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was centrifuged for 10 minutes at 25,000g and the lysate supernatant was incubated for 3 hours with 1 mL of glutathione sepharose beads (GE Healthcare) pre-washed in lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... the purified OxlT was mixed with purified D5901-A08-Fab at a 1:1.3 molar ratio at 4 °C overnight and applied to a HiLoad 16/60 Superdex200 pg column (GE healthcare) using buffer D (20 mM MES-KOH ...
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bioRxiv - Biochemistry 2021Quote: PeSkC and RoSBC samples were applied to a 1.8×13cm Sephadex G100 column at a flow rate of 1ml min-1 using an ÄKTAprime plus unit (GE Healthcare). Void volume was calculated by running blue dextran ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at 1:10,000 and blots developed with ChemiGlow detection reagent (ProteinSimple) and visualized using ImageQuant LAS 4000 chemiluminescent camera (GE Healthcare). For RNA-Immunoprecipitations FLAG-Immunoprecipitation from DHX34-GFP-FLAG A5 cells were performed with in NET2 buffer (50 mM Tris-HCl pH7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... the coating was done with a 2:1 ratio of non-coupled:Cy3-coupled fibronectin prepared with Cy3 Mono-Reactive Dye Pack (GE Healthcare) as recommended by the manufacturer.
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bioRxiv - Biochemistry 2019Quote: ... 25 mM Tris-HCl pH 7.5) prior to adding to a HiTrap QFF (1 mL) strong anion exchange column (GE Healthcare) equilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2020Quote: Solid-phase synthesis of oligoribonucleotide was performed on a 1 μmol scale using AKTA Oligopilot plus 10 synthesizer (GE Healthcare) and Custom PrimerSupport™ Ribo A 40 (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... FANCD2 and FANCI at 1 µM were sequentially run on a Superdex 200 10/300 column (GE Healthcare Life Sciences) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Western blot of the immunoprecipitated recombinant proteins was performed as described in the above section using anti-GST HRP (horseradish peroxidase) conjugate (1:10,000 Amersham GE Healthcare) for 60 minutes at room temperature under agitation ...
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bioRxiv - Biophysics 2020Quote: ... A volume of 50 μl of each sample at 3 mg·ml−1 was loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 25 mM Hepes pH 7.8 ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by centrifugation (35,000 rpm, 40 minutes, 4°C) and the supernatants loaded onto 1 mL HisTRAP HP (GE Healthcare) columns equilibrated in lysis buffer (buffer A) ...
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... The extracellular media was then diluted two-fold with 20 mM HEPES buffer at pH 7.0 and applied to 1 column volume (CV) of DEAE Sepharose Fast Flow Resin (from GE Healthcare) equilibrated with 10 CV of 20 mM HEPES buffer at pH 7.0 (1 CV equaled 2 ml of settled resin for 25 ml cultures and 1 CV equaled 4 ml of settled resin for 250 ml cultures) ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mL of the clarified lysate was loaded onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare, Illinois) charged with Ni2+ and preequilibrated with buffer A ...
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bioRxiv - Bioengineering 2020Quote: ... and supernatant was filtered with a 0.22 μm filter before loading onto a 1 ml HisTrap HP column (GE Healthcare). Proteins bound to the column were washed with 10 column volumes of washing buffer (50 mM Tris ...
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bioRxiv - Physiology 2021Quote: ... The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (GE Healthcare, Buckinghamshire, UK; dilution: 1:5000) or anti-mouse IgG (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... and transferred by western blotting (30V, 1 hour) to polyvinylidene fluoride membrane (P 0.45 μm; 10600023 Amersham Hybond, GE Healthcare). Blots were probed with biotinylated GNA lectin (40 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... reverse-crosslinked samples were combined and the DNA was purified using solid phase reverse immobilization (SPRI) Sera-Mag SpeedBeads (1.6:1 ratio, GE Healthcare). The final library was eluted with 22 μL ddH2O.
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bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
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bioRxiv - Microbiology 2020Quote: ... Swarmer cells were isolated from the culture by centrifugation (20 mins at 11,000 rpm, 4°C) after mixture with 1 volume of Percoll (GE Healthcare). The synchronized swarmer cells were pipetted onto an agarose (2% ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A second purification step was performed by size-exclusion chromatography (SEC) with Superdex 75 column (1 x 30) (GE Healthcare) at a flow rate of 0.5 mL/min monitored at 280 nm using a 25 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified and the supernatant filtered through 0.22 μm filters and subjected to nickel affinity chromatography using 1 ml HisTrap HP columns (GE Healthcare). Elution was carried out via a 25-500 mM imidazole gradient in 50 mM Tris [pH 8] ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... The sequence encoding mouse LRRC23 (residue 171-340 aa, CCDS20528.1) was cloned and inserted into pGEX6p-1 expression vector (GE healthcare), followed by transformation into E ...
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bioRxiv - Biophysics 2022Quote: ... at a 1:3 protein:dye molar ratio for 1 h at room temperature and then desalted on a PD-10 desalting column (GE Healthcare), where the free dye and the conjugated Alexa488-αSOs were separated ...
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bioRxiv - Cancer Biology 2022Quote: ... phospho-p38 MAPK (Invitrogen, #36-8500, 1:1000. Chemiluminescence was recorded using the Image Quant Las4000 imaging system (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation (15’000 xg, 4 °C, 30 minutes) and supernatants were incubated with 1 ml Glutathione Sepharose (GE Healthcare) for 2 hours at 4 °C ...