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Citations for GE Life Sciences :
451 - 500 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by size exclusion chromatography (Hi-load 16/60 Superdex 200 column, GE Healthcare Life Sciences) in a buffer (20 mM Tris-HCl ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... Recombinant His-tagged proteins (MA4376, MA4376D54N and MA4375) were purified with the ÄKTAprime plus system using HisTrap HP (GE healthcare) columns ...
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bioRxiv - Developmental Biology 2021Quote: ... and proteins blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences). The membranes were then blocked with 5% skimmed milk in Tris-Buffered Saline Tween (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran 0.45 μm NC; GE Healthcare) at 100 V for 180 min on ice in the transfer buffer without SDS/EDTA ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then transferred to nitrocellulose membranes (Amersham; 0.45 µM NC; GE Healthcare) at 360 mA for one 1 h or 30 mA overnight in blotting buffer (10% v/v 10x running buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were then transferred onto a nitrocellulose membrane (GE Healthcare, Little Chalfont, UK) for 1 hr using a Trans–Blot device for semi-dry transfer (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... The target protein on the membrane was visualized with ECL Select (GE Healthcare) and a Luminescent Image Analyzer LAS-3000 Mini (Fujifilm).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Hybond-P; GE Healthcare, Wauwatosa, WI, USA) and decorated with antibodies against GFP (ab290 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Hybond-P; GE Healthcare, Wauwatosa, WI, USA). Ponceau S was used to assess equal loading of the insoluble fraction ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Hybond-P; GE Healthcare, Wauwatosa, WI, USA) and decorated with antibodies against GFP (ab290 ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were blotted onto a 0.2 μm Protran™ nitrocellulose membrane (GE healthcare) using 1x transfer buffer (3.03 g/L Tris base ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to 0.2 µm nitrocellulose membranes (GE Healthcare Life science, Germany) in a semi-dry transfer system (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were transferred on 0.45μm nitrocellulose membrane (10600002 Amersham, GE Healthcare). The membrane was blocked with 5% BSA in PBS for one hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The target protein on the membrane was visualized with ECL Select (GE Healthcare) and a Luminescent Image Analyzer LAS-3000 Mini (Fujifilm).
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a methanol-activated Amersham Hybond PVDF membrane (GE Healthcare) at 100 V for 1 h at room temperature using Mini Trans-Blot Cell system (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... affinity-purified proteins were spotted onto Protran nitrocellulose membranes (0.45 μm, GE Healthcare). After being dried ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were then transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare), and the membrane was blocked with 5% non-fat dry milk for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto PVDF membranes (Amersham Hybond 0.45 µm, GE Healthcare). For immunoblotting ...
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bioRxiv - Pathology 2020Quote: Immunoblotting was performed after protein transfer onto Hybond-P membranes (GE Healthcare, RPN303F). After blocking membrane nonspecific binding sites in TBS (20 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on SDS– polyacrylamide gel were transferred onto a nitrocellulose membrane (GE Healthcare). The bands were visualized utilizing Enhanced Chemiluminescence (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to nitrocellulose membranes (GE Healthcare Life Sciences – REF 10600003), which were incubated with the appropriate antibodies according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μm, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Immunology 2022Quote: ... The separated proteins were transferred on Amersham Protran nitrocellulose membrane (GE Healthcare Lifescience). After blocking with 5 % skimmed milk in TBS-T (0.1 % Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane (Whatman, GE Healthcare Life Sciences), which was blocked with 5% dry-milk-Tris Buffer saline ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were transferred onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare) and an anti-His-HRP conjugate antibody (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μM, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Cell Biology 2023Quote: Recombinant RAD51 proteins were spotted on nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). Membranes saturation ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred to a 0.45 μm pore size nitrocellulose membrane (GE Healthcare) and incubated with rabbit polyclonal anti-Hfq antibody (Andrew Feig ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred onto a 0.45μm nitrocellulose blotting membrane (#10600002; GE Healthcare) using wet electrotransfer for 1h at 110V ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred onto 0.2 μm pore size nitrocellulose membranes (GE Healthcare, 10600001) at 18 V for 1 h using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to Cytiva Amersham Hybond PVDF membranes (formerly GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Then proteins were transferred to a nitrocellulose membrane (Whatman, GE Healthcare Life Sciences). After transfer ...
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bioRxiv - Neuroscience 2024Quote: ... recombinant IgLON5 or LG1 protein was spotted onto a nitrocellulose membrane (GE Healthcare), membranes were blocked with 1% BSA in PBS ...
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bioRxiv - Microbiology 2022Quote: ... and His-trap column (GE healthcare) previously equilibrated with Lysis Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Biophysics 2023Quote: ... Hi-Trap Heparin HP (GE Healthcare), and Superdex 200 10/300 GL columns (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The tag-less protein was then purified by size-exclusion chromatography (SEC) using a Superdex 200 increase column (GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Genetics 2020Quote: ... followed by purification of the tag-free protein by anion exchange chromatography on a HiTrap Q XL column (GE Healthcare) and nickel affinity chromatography ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... the bound fusion protein was eluted with 20– 200 mM imidazole gradient using a FPLC purifier system (ÄKTA; GE Healthcare). Peak fractions were pooled and digested with SENP2 protease (at final 0.01 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Each GST-fusion protein was released from GST adsorbed to glutathione-sepharose by PreScission protease (GE Healthcare, 27-0843-01). The concentrations of purified proteins were determined by Bradford analysis (Bio-Rad 5000006) ...
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bioRxiv - Cell Biology 2019Quote: ... cleaved MBP-6X-His and 8X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... cleaved MBP-6X-His and 6X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Size exclusion chromatography was performed to separate FLAG-Ddi1 from the cleaved SUMO protein or the full-length His-SUMO-FLAG-Ddi1 using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) in a buffer of 20 mM Tris HCl pH 7.5 ...