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Citations for GE Life Sciences :
701 - 750 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: Binding affinity characterizations of transcribed RNA biosensors were performed using a SPR assay on the Biacore X100 instrument from GE Healthcare (Chicago, IL, USA) as previously described8 ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a hydrophobic HiTrap Butyl HP 1 ml column (GE healthcare life sciences), pre-equilibrated with 5 column volumes of buffer AButyl (500 mM ammonium sulfate ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies for chemiluminescence detection were donkey anti-rabbit-HRP (GE healthcare, NA 9340V, 1:5000) or sheep anti-mouse-HRP (GE healthcare ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies were detected using mouse IgG HRP linked whole Ab (1:4000; NXA931; GE healthcare).
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using a ChemiDoc imaging system (BioRad) ...
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bioRxiv - Biochemistry 2022Quote: ... either a 1:10,000 dilution of α-mouse-HRP conjugated antibody (GE Healthcare Life Sciences NA931) or a 1:2,000 dilution of α-mouse-HRP conjugated antibody (used for western blot of immunoprecipitations ...
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bioRxiv - Neuroscience 2022Quote: ... and reincubated with the secondary HRP-conjugated antibody (1:2000 dilution for anti-rabbit, GE Healthcare, NA934 ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated for 1 h with ECL mouse IgG HRP-conjugated whole antibody (GE Healthcare) or rabbit IgG HRP-conjugated whole antibody (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... sheep anti-mouse horseradish peroxidase-linked antibody (1:3000, NA931V, GE Healthcare Life Sciences, Uppsala, Sweden), donkey anti-rabbit horseradish peroxidase-linked antibody (1:4000 ...
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bioRxiv - Molecular Biology 2019Quote: ... donkey anti-rabbit horseradish peroxidase-linked antibody (1:4000, NA934V, GE Healthcare Life Sciences, Uppsala, Sweden).
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bioRxiv - Cancer Biology 2019Quote: ... and then incubated for two hours in secondary antibody – anti-rabbit-HRP (1:5000, GE Healthcare) or anti-mouse-HRP (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 1 h at room temperature in the appropriate HRP-labelled secondary antibody (GE Healthcare). Blots were developed with ECL Clarity (GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with Anti-mouse HPR-conjugated secondary antibody (1:5000 dilution, GE Healthcare) at RT for 2 hours and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody was detected with HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-liked sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized using Radiance Plus chemiluminescent HRP substrate (Azure Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized as described above.
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL (GE healthcare NA931, NA934).
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used: sheep anti-mouse (HRP-conjugated, GE Healthcare, NXA931V, 1:10000), donkey anti-rabbit (HRP-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using Chemidoc Molecular Imaging System (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies were used as follows: Horseradish peroxidase (HRP)-conjugated mouse IgG (1:10,000; GE Healthcare) or rabbit IgG (1:3000 ...
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bioRxiv - Microbiology 2019Quote: ... the protein was purified using HisTrap HP histidine-tagged protein columns (GE healthcare, Chicago, IL, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with protein A or protein G Sepharose beads (GE Healthcare, Milwaukee, WI, USA) for 3 h with gentle rocking in the cold room ...
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bioRxiv - Immunology 2020Quote: ... The IgG was then purified using protein G affinity chromatography (HiTrap Protein G HP, GE Healthcare) and size-exclusion chromatography (Superdex 200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were exchanged into protein storage buffer on an NAP-5 column (GE Healthcare, 17085301), concentrated with a 50 kDa MWCO Amicon Ultra-0.5 device (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Large scale protein purification was done using ÄKTA prime Fast Liquid Protein Chromatography System (GE Healthcare). DE droplets were analyzed on a BD LSR Fortessa SORP (Special Order Research Product ...
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bioRxiv - Immunology 2021Quote: ... and the protein within the supernatant was purified with a Protein A-Sepharose column (GE Healthcare) according to the manufacturer’s instruction for primary purification ...
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bioRxiv - Microbiology 2022Quote: ... and isolated from supernatants by affinity chromatography using Protein A or Protein G columns (GE Healthcare). MAbs were eluted with 0.1 M glycine at low pH and dialyzed into PBS.
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bioRxiv - Microbiology 2024Quote: ... The mutated proteins were purified from the media using a Protein-A affinity column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Protein G sepharose beads (GE Healthcare) were washed three times in lysis buffer to remove any residual ethanol from the storage buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and Protein G Sepharose (GE Healthcare) pre-blocked in 1% BSA-PBS were used to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein-G sepharose beads (GE Healthcare) were spun at 13000X g for 1 minute ...