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Citations for GE Life Sciences :
751 - 800 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Proteins were further purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE Healthcare) integrated in the NGC system ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were revealed by mean of enhanced chemiluminescence (GE Healthcare) in a BioRad ChemiDoc XRS+ ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was loaded onto a pre-equilibrated (with low imidazole buffer) HisTrap™excel column (GE Healthcare). Following a wash step of the column with 5 column volumes low imidazole buffer ...
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bioRxiv - Microbiology 2024Quote: ... or a HiLoad 16/60 Superdex 75 pg column (GE Healthcare) pre-equilibrated in SEC-buffer (25 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Concentrated proteins were further purified by Size-exclusion chromatography (SEC) either using a HiLoad 16/60 Superdex 200 pg column (GE Healthcare) or a HiLoad 16/60 Superdex 75 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Images were acquired as Z-stacks on a DeltaVision Core microscope (Applied Precision) using the 100x objective and images were processed and deconvolved using the SoftWoRx and FIJI software.
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bioRxiv - Microbiology 2024Quote: ... 30 min) and loaded onto a 5 ml HighTrap MBP column (Cytiva) using an Äkta-Pure system (GE Healthcare). The column was washed with 10 column volumes of lysis buffer and protein eluted with MalE-elution buffer (100 mM Tris-HCl pH7.2 ...
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bioRxiv - Microbiology 2024Quote: ... The elution fractions containing MalE-DmxA variants were pooled and loaded on a HiLoad 16/600 Superdex 200pg (GE Healthcare) SEC column ...
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bioRxiv - Microbiology 2024Quote: ... The resulting lysate underwent clarification through centrifugation (125,000g for 30 minutes at 277 K) using a Ti-45 rotor (Beckmann) and was subsequently applied to a 1 ml HisTrap HP column (GE Healthcare). The column underwent an initial wash with five column volumes of lysis buffer containing 40 mM imidazole at pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... and then subjected to size-exclusion chromatography using either an S75/26–60 column or an S200/26–60 (GE Healthcare) in the same buffer as before but without imidazole ...
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bioRxiv - Microbiology 2024Quote: ... and an Amersham Imager 600 (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2024Quote: ... for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T, GE Healthcare, code NA934V); for EF1-α - anti-mouse (1:10,000 in 1% milk/PBS-T ...
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bioRxiv - Microbiology 2024Quote: Cell-free supernatants were separated by Superdex 30 (GE Healthcare) size exclusion column equilibrated with distilled water and separated at 0.25 ml min-1 ...
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bioRxiv - Microbiology 2024Quote: The same mixture of phage DNA as mentioned above was amplified by Multiple Displacement Amplification (MDA) using the Genomephi V3 kit (GE Healthcare Life Science, Marlborough, MA, USA) with 10 ng input and incubated at 30°C for 0.5 h (MDA_0.5h ...
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bioRxiv - Immunology 2024Quote: ... Lysate pre-cleaning steps were performed using Protein G Sepharose beads (GE Healthcare). To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... together with horseradish peroxidase conjugated sheep anti-mouse immunoglobulin G (GE Healthcare) as a secondary antibody (dilution 1:2000) ...
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bioRxiv - Immunology 2024Quote: ... at room temperature for 1 hour before visualization using the ECL Detection kit (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... Proteins were dialyzed against 10 mM Tris (pH 8) and purified by ion exchange (Q column) followed by size exclusion (S200, GE Healthcare) chromatography ...
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bioRxiv - Immunology 2024Quote: ... CM5 chips (GE Healthcare) were coated in the HLA-I–specific mAb W6/32 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... or 33% Percoll (GE Healthcare) as previously described(7) ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... SPR binding experiments were performed with a BIAcore 3000 instrument and analyzed with BIAevaluation software v4.1 (GE Healthcare). CM5 chips (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1 without tag was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer B containing 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The purified sample was further separated by HiLoad 16/600 Superdex 200 pg (GE Healthcare, 120 mL) (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was loaded to 5 mL HisTrap Fast flow column (GE Healthcare) pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... the eluant was finally subjected to two rounds of gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer A containing 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
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bioRxiv - Immunology 2024Quote: ... Filtered suspensions were centrifuged at 220g for 10 minutes and resuspended in 5mL of 22% Percoll (GE Healthcare) in Myelin Gradient Buffer (5.6mM NaH2PO4•H2O ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences; Table S4).
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bioRxiv - Cell Biology 2024Quote: ... or a Personal Deltavision (Applied Precision, LLC) microscope equipped with a CoolSNAP HQ2 camera (Photometrics ...
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bioRxiv - Cell Biology 2024Quote: ... These eluted proteins were subsequently passed through a superdex s75 16/60 column attached to an AKTA Pure system (GE healthcare) in 50 mM Tris pH 8.0 and 300 mM NaCl.
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bioRxiv - Microbiology 2024Quote: ... 60 × 1.6 cm size exclusion column (GE Healthcare) in FPLC (AKTA Pharmacia) ...
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bioRxiv - Microbiology 2024Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare) on an iBright FL1500 (ThermoFisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... The His-tag S protein was purified by gel filtration using the Akta explorer 100 system with a high-load Superdex pg 200 16/600 column (GE Life Sciences, USA). Main peak fraction was collected ...
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bioRxiv - Microbiology 2024Quote: ... Parasite cultures were synchronised by Percoll (GE Healthcare) for isolation of mature schizont stages parasites ...
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bioRxiv - Microbiology 2024Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare) and quantified with the program ImageQuant (version 8.2 ...
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bioRxiv - Microbiology 2024Quote: ... the samples were loaded on a Hi-Load Superdex 200 16/600 column (GE Healthcare) equilibrated with 50mM Tris-HCl pH7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was loaded into a 1ml HisTrap column (GE Healthcare) and the bound proteins were eluted in 50mM Tris-HCL pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... using AKTA-FPLC system (GE healthcare)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blot image was acquired by ImageQuant LAS 4000 (GE Healthcare) or Fusion FX (Vilber) ...
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bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
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bioRxiv - Microbiology 2024Quote: ... before loading on a HiTrap MabSelect SuRe 5 ml column (GE Healthcare), equilibrated with McIlvaine buffer pH 7.2 (174 mM Na2HPO4 ...
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bioRxiv - Microbiology 2024Quote: ... was injected onto a Superdex200 10/300 GL Increase SEC column (GE Healthcare) equilibrated with sample buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The supernatants were directly collected and filtered through 0.45 µm PTFE membrane filters (GE Healthcare Life Sciences, UK) into amber vials and stored at -20°C until analyses ...
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bioRxiv - Molecular Biology 2024Quote: ... Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) was used for chemiluminescent protein detection.
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bioRxiv - Microbiology 2024Quote: ... The mutated proteins were purified from the media using a Protein-A affinity column (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... on Akta primeplus FPLC system (GE Healthcare). Cell pellets were thawed on ice and resuspended in buffer A (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... and further purified by size exclusion chromatography (HiLoad 26/600 Superdex 200, GE Healthcare), pre-equilibrated with storage buffer E (20 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...