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Citations for GE Life Sciences :
5201 - 5250 of 5568 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... while the secondary antibody (1/10,000) was a commercial Amersham ECL-HRP linked rabbit IgG (GE Healthcare).
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bioRxiv - Immunology 2021Quote: Purified antibodies targeting S1 were quantified with SPR assay using the BIAcore 8000 system (GE Healthcare, USA) carried out at 25°C in single-cycle mode ...
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bioRxiv - Immunology 2020Quote: ... and immunodetected using appropriate primary and peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Pittsburg, PA, USA). Proteins were visualized by West Pico and West Dura chemiluminescence Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Immunology 2024Quote: ... The bispecific antibody was further polished by cation exchange chromatography using Capto MMC (GE Healthcare, Princeton, NJ) and ion exchange chromatography using Q HP (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies were used together with horseradish peroxidase-conjugated α-mouse immunoglobulin G (1:5000, GE Healthcare) as secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... antibodies were then incubated for 1 hour and revealed with the enhanced ECL detection system (GE Healthcare). The primary antibody against P2X7 (#APR-004 ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antibodies were purified by size exclusion chromatography on a Superdex 200 26/600 column (GE Healthcare) in 25 mM Citrate ...
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bioRxiv - Genetics 2024Quote: ... Incubation with secondary antibodies for 1.5 hours was followed by chemiluminescence development system (GE Healthcare, Pittsburgh, USA). Antibodies details are listed in Supplemental Table 2-3.
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were centrifuged at 16,000 x g for 20 minutes and the supernatant used for affinity purification of biotinylated proteins using 25 µl of washed streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301) for 16 hours with rotation at 4℃ ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were centrifuged at 15,000 × g for 15 minutes and the supernatant was used for biotinylated protein capture using 15 μl of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... A second purification step was performed for IDO-Gal3 using size exclusion chromatography in an ÄKTA Pure Protein Purification System (GE Life Sciences, Marlborough, MA, USA). Molecular weight and purity were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were injected and purified on SEC (size exclusion chromatography) column Superdex 200 10/300 GL (GE Healthcare Life Sciences, Piscataway, NJ, USA) against a buffer containing 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was further purified by size exclusion chromatography using HiLoad® 16/600 Superdex® 75 pg column (GE Life Sciences, 28-9893-33) equilibrated with 1x Tris buffer (50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... The protein samples were then applied to size exclusion chromatography (Superdex 200 prep grad, HiLoad 26/60 or 16/60, GE Healthcare Life Sciences, Freiburg, Germany) using 50 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Plant Biology 2024Quote: ... His-tagged recombinant proteins were overexpressed in Escherichia coli strain Rosetta™ (DE3) and purified via an Äkta chromatography system using HisTrap columns (GE Healthcare, Waukesha, WI, USA). Washing (20 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... ECL Anti-Mouse IgG HRP linked secondary 1:5000 (GE Healthcare #NA931); ECL Anti-Rabbit IgG HRP linked secondary 1:5000 (GE Healthcare #NA934).
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bioRxiv - Cell Biology 2020Quote: ... ECL Anti-Rabbit IgG HRP linked secondary 1:5000 (GE Healthcare #NA934).
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bioRxiv - Neuroscience 2021Quote: ... or sheep anti-mouse IgG (GE Healthcare, cat #NA931, RRID:AB_772210, 1:10,000) in TBS-T containing 5% milk for 2 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... goat polyclonal anti-GST (GE healthcare, 27-4577-01; 1:2000, IB); rabbit polyclonal anti-Cap1 [1 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... HRP-linked donkey anti-rabbit IgG (NA934, GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Molecular Biology 2020Quote: ... and ECL Donkey anti-rabbit IgG-HRP (1:3000, GE Healthcare NA9340V). Immunoblots were developed using Clarity Western ECL substrate (BioRad).
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bioRxiv - Developmental Biology 2019Quote: ... HRP-conjugated secondary (1:10000 donkey anti-Rabbit-HRP, GE Healthcare #NA934V) and Amersham ECL prime detection kit (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: ... HRP/anti-CM13 monoclonal conjugate was from GE Healthcare (Boston, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or horseradish peroxidase-conjugated donkey anti-rabbit IgG (GE Healthcare, München, Germany)) was incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Western blots were incubated in secondary donkey anti-mouse (GE Healthcare NA934V) or donkey anti-rabbit (GE Healthcare NXA931 or Jackson Immunoresearch 711-035-152 ...
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bioRxiv - Microbiology 2022Quote: ... Secondary anti-mouse or -rabbit IgG HRP conjugates (GE Healthcare, Piscataway, NJ) were used in immunoblotting according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and anti-rabbit-HRP in a dilution of 1:2000 (GE Healthcare), mouse anti-CDC2 at 1:1000 dilution (αPSTAIR ...
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bioRxiv - Microbiology 2024Quote: ... together with horseradish peroxidase conjugated sheep anti-mouse immunoglobulin G (GE Healthcare) as a secondary antibody (dilution 1:2000) ...
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-Rabbit IgG-HRP (1:2,000, GE Healthcare, Cat# NA934, RRID:AB_772206), goat anti-Rabbit IgG IRDeye® 800CW (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sheep anti-Mouse IgG-HRP (1:3,000, GE Healthcare, Cat# NXA931, RRID:AB_772209), donkey anti-Rabbit IgG-HRP (1:2,000 ...
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bioRxiv - Biochemistry 2024Quote: ... HRP-conjugated anti-rabbit IgG (1:2500, NA934V, GE Healthcare, Fairfield, CT) and HRP-conjugated anti-mouse IgG (1:2500 ...
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bioRxiv - Microbiology 2023Quote: ... anti-rabbit IgG conjugated to horseradish peroxidase (GE healthcare NA93AV, 1:10,000), or Licor IR Dye800 CW goat anti-rabbit (926–32,211 ...
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bioRxiv - Developmental Biology 2023Quote: ... and donkey anti-rabbit IgG HRP-linked (1:2000; NA934, GE Healthcare)] and visualized on an Amersham Imager 600 (29083461 ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-linked sheep anti– mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-liked sheep anti-mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Cancer Biology 2023Quote: ... Donkey anti- Rabbit IgG (GE Healthcare Life Sciences, Cat. No. NA934-1ML) or Donkey anti-Goat IgG (ThermoFisher ...