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Citations for GE Life Sciences :
5101 - 5150 of 5568 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and HRP-conjugated anti-rabbit IgG (GE Healthcare, NA934V, 1:5000).
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated sheep anti-mouse IgG (GE Healthcare NA931, 1:5000), HRP-conjugated goat anti-rabbit IgG (Cedarlane #111-035-144 ...
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bioRxiv - Molecular Biology 2020Quote: ... ECL Sheep anti-mouse IgG-HRP (1:3000 GE Healthcare NA9310V) and ECL Donkey anti-rabbit IgG-HRP (1:3000 ...
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bioRxiv - Developmental Biology 2019Quote: ... HRP-conjugated donkey anti-rabbit IgG (GE Healthcare, NA934V, lot 9780721), Alexa FluorTM 594-conjugated anti-guinea pig (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated with 1:2000 anti-M13 pVIII-HRP (GE Healthcare) in PBS-T 5% milk for another hour ...
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bioRxiv - Genetics 2021Quote: ... incubated with HRP-conjugated anti-mouse (GE Healthcare, #NXA931, 1:5000) and imaged in a Bio-Rad ChemiDoc imaging system.
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bioRxiv - Biophysics 2020Quote: ... anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare) for one hour ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Sheep-anti-mouse coupled to HRP (1:2000) (GENA931, GE Healthcare) was used as secondary antibody.
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bioRxiv - Cell Biology 2022Quote: ... and ECL anti-mouse IgG horseradish peroxidase-linked (GE Healthcare; NA931V).
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bioRxiv - Cell Biology 2022Quote: ... anti-rabbit IgG–horseradish peroxidase (GE Healthcare, NA934V or CST, #7074), Alexa Fluor 488 anti-Mouse IgG1 (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... or sheep anti-mouse immunoglobulin (GE Healthcare; NA931-1ML; 1:10000) and the enhanced chemiluminescence (ECL ...
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bioRxiv - Genetics 2022Quote: ... and then incubated with goat-developed anti-rabbit (GE Healthcare, NA934) or goat-developed anti-mouse immunoglobulin G (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated with anti-mouse IgG (1:10,000; GE Healthcare) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... HRP–conjugated donkey anti-rabbit IgG (NA934, GE Healthcare, 1:20000), biotin-conjugated donkey anti-rabbit IgG (E0432 ...
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bioRxiv - Cell Biology 2024Quote: Horseradish Peroxidase (HRP)-conjugated anti-mouse IgG (GE Healthcare, Buckinghamshire, UK)
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bioRxiv - Molecular Biology 2024Quote: ... HRP-conjugated sheep anti-mouse IgG (1:5000; GE Healthcare, NA931), HRP-conjugated goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... followed by anti-rabbit IgG-HRP (1/2000, GE Healthcare, NA934V) and developed using Pierce™ ECL Western Blotting Substrate (Thermofisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... anti-mouse/-rabbit-IgG-HRP (Donkey polyclonal, GE Healthcare, Freiburg, DE) were used as secondary antibodies.
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bioRxiv - Microbiology 2022Quote: ... or anti-rabbit-HRP (NA9340, GE Healthcare, Thermo Fisher Scientific Inc.) secondary antibodies were used (1:10000) ...
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bioRxiv - Cell Biology 2023Quote: ... sheep anti-mouse IgG-HRP (GE Healthcare, Chicago, IL; catalog # NA931V) was used at 1/5,000.
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bioRxiv - Microbiology 2023Quote: ... 000 dilution and HRP Goat anti-rabbit IgG (GE Healthcare, NA934) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before[30] ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 µL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 µL per 1 mg digested mAb ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated in standard procedures using 12% polyacrylamide gel (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Life science, Germany) using TransBlot semi dry transfer cell (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 50 mM imidazole) before eluting the protein (HBS, 250 mM imidazole) following desalting using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium) to remove imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... Equivalent amounts of protein or equal volume from each sample were run on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (GE Healthcare, Piscataway, NJ, USA). Following blocking in 5% milk or bovine serum albumin the blots were incubated with primary antibodies and analyzed by HRP-conjugated secondary antibodies (Jackson Laboratories) ...
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bioRxiv - Pathology 2019Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Microbiology 2019Quote: ... 75-90 μl of affinity-purified protein sample was loaded onto a Superdex 200 Increase 10/300 GL SEC column (GE Healthcare, Chicago, IL, USA) previously equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The reacting agents were then separated from the now methylated proteins by size-exclusion chromatography using NapTM-5 columns according to the manufacturer’s instructions (GE Healthcare Life Sciences, Amersham, UK).
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were further resolved using size exclusion chromatography on gel filtration columns using the Superdex 200 (16/600) (GE Healthcare, GE28-9893-35) or Superdex 75 (16/600 ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were incubated in Bjerrum Schafer-Nielsen transfer buffer and proteins were transferred to a water-activated nitrocellulose membranes (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Sunnyvale, USA) using semi-dry transfer (Trans-Blot® Turbo™ ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were incubated in Bjerrum Schafer-Nielsen transfer buffer and proteins were transferred to a water-activated nitrocellulose membranes (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Sunnyvale, USA) using semi-dry transfer (Trans-Blot® Turbo™ ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP-tagged proteins were filtered on a Superdex 200 10/300 GL or Superose 6 Increase 10/300 GL column (GE Healthcare, Little Chalfont, UK) and then labeled with SNAP-surface-549 (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was further purified by size-exclusion chromatography (SEC) using a sizeexclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2021Quote: Nickel Immobilised Metal Ion Affinity chromatography (Ni2+-IMAC) for protein purification was conducted using a HisTrap® HP 5 mL column (GE Healthcare, Munich, Germany) coupled to a BioLogic DuoFlow™ Chromatography System (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Biophysics 2021Quote: ... The SΔTM protein was then further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) (Fig ...
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bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... 15-25 μg of total protein per sample were resolved on 8%-14% SDS-PAGE and transferred onto nitrocellulose membrane (Amersham Hybond, GE Healthcare Life Science, 10600002). Membranes were blocked in 5% milk in PBS followed by incubation with specific primary and secondary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Protein was separated on the 10 % SDS PAGE and transferred on the polyvinylidene difluoride (PVDF) membranes (Hybond™-P; GE Healthcare, Piscataway, NJ, USA) using the wet transfer method (1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Following electrophoresis, protein was transferred onto a 0.45 μm nitrocellulose (Amersham Protran Premium, 10600096) or a PVDF membrane (GE Healthcare Amersham Hybond ECL, RPN68D). 0.4% PFA was used to fix the protein on the membrane ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Pathology 2019Quote: ... Equal amounts of protein extracts (25 µg) were separated by SDS-PAGE before electrophoretic transfer onto a nitrocellulose membrane (GE Healthcare, Velizy-Villacoublay, France). Western-blot analysis was carried out using anti-LC3-II (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to nitrocellulose membranes and visualized by enhanced chemiluminescence using ECL Prime Western Blotting Detection Reagent (#RPN2232, GE Healthcare; Chicago, IL, USA) and imager ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Synthetic Biology 2019Quote: ... A Bio-Rad ChemiDoc XRS imaging system was used for visualizing the protein blot after incubations with ECL plus reagent (GE Healthcare, catalog no. RPN2132).
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Pathology 2020Quote: ... Equal volumes of plasma were pooled from three mice and lipoproteins were fractionated by fast protein liquid chromatography (ÄKTA pure FPLC system, GE Healthcare, Pittsburgh, PA, USA) and triglyceride and cholesterol in fractions quantified with reagent kits (Wako Diagnostics ...
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bioRxiv - Synthetic Biology 2019Quote: ... between 5 and 10 mg protein ml−1) was pipetted into the measurement cell of a MicroCal ITC200 isothermal titration calorimetry instrument (GE Healthcare Life Sciences, USA). To avoid potential further unfolding of mutant protein we directly analysed them in imidazole-containing buffer A without previous dialysis ...