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Citations for GE Life Sciences :
5151 - 5200 of 5568 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cellular lysates were cleared by centrifugation and 300 μg of total protein were added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, Chicago, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Rv1339 protein was visualized with a FujiFilm LAS-3000 Image Reader and the Amersham™ ECL™ Western Blotting Analysis system (GE Healthcare, UK).
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bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 μL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were electrophoresed on an SDS polyacrylamide gel and then transferred to a nitrocellulose membrane (Amersham Protran Premium; GE Healthcare, Waukesha, WI, USA) using a semidry method ...
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bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a Superdex® 200 Increase 10/300 GL on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with PBS (pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... The labelled protein was purified from the excess of free dye by a P10 desalting column with Sephadex G25 matrix (GE Healthcare, Waukesha, WI, USA), divided into aliquots ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before(60) ...
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bioRxiv - Neuroscience 2023Quote: ... A small volume of the bead-coupled GST-fusion proteins was loaded into a ProbeQuant G-50 Micro Column (GE Healthcare, cat no. 28903408) and washed once in ice cold lysis buffer containing 1 % (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were subjected to SDS-PAGE (15 μg per lane) and blotted onto Protran Nitrocellulose Mem-brane-Whatman (GE Healthcare Life Science, USA). Membranes were blocked with Blocking Solution (5% powdered milk in TBST ...
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bioRxiv - Microbiology 2023Quote: ... aMCFCA was then purified from the supernatant using the AKTA protein purification system through a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02) with loading buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with a linear gradient of imidazole (0–1,000 mM) in His-tag-binding buffer using the ÄKTA FPLC™ Fast Protein Liquid Chromatography system (GE Healthcare, Princeton, NJ, USA). The eluted protein peak was collected and dialyzed against buffer D containing 50 mM Tris–HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was diluted to 100mM NaCl and clarified by centrifugation before application to a 5mL Heparin HiTrap HP (GE Healthcare 17-0407-01) column equilibrated with Buffer B (25mM HEPES pH 7.5 ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Physiology 2023Quote: ... Immunoreactive bands were detected using a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after the membranes were incubated with ECL Prime Western Blotting Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analysis of the immunoreactive bands was performed using the FluorChem FC3 Analysis Software.
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before[62] ...
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bioRxiv - Biophysics 2024Quote: ... Dyes were incubated with G-actin at a 2-fold molar excess for 2 hours at room temperature before being separated from the labeled protein by applying the labeling reaction to a spin column packed with Sephadex G-50 Fine DNA Grade (GE Healthcare GE17-0573-01) hydrated with A buffer (5 mM Tris-HCL (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... and the flow-through sample containing the ASK1 TBD protein was purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM HEPES (pH 7.0) ...
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bioRxiv - Neuroscience 2022Quote: ... For AKT1 detection, anti-AKT (pan) (11E7) (Cell Signalling Technology, #4685, 1:1000 in 5% BSA) and donkey-anti-rabbit IG (GE Healthcare, NA9340V, 1:3000 in 5% BSA) antibodies were used ...
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bioRxiv - Molecular Biology 2020Quote: ... the antibodies were detected by using the Amersham ECL Prime Western Blotting Detection Reagent (RPN2232; GE Healthcare). The primary antibody was NSUN6 (1:500 ...
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bioRxiv - Immunology 2019Quote: ... Columns were prepared by coupling Y-3 and B22-249 antibodies to CNBr-activated Sepharose (GE Healthcare) (1 mg antibody/40 mg Sepharose) ...
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bioRxiv - Cell Biology 2019Quote: ... The bound secondary antibodies were detected using the enhanced chemiluminescence (ECL) detection reagents (GE Healthcare, Piscataway, NJ). Blots were imaged and quantified using the Quantity-OneTM or Image LabTM software (Bio-Rad) ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then incubated for 75 min with the secondary antibody coupled to horseradish peroxidase (GE Healthcare). After four washing steps with TBST (5 min each) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HRP conjugated secondary antibodies were used at 1:10,000 and visualised using ECL (GE Healthcare, Buckinghamshire, UK), high performance chemiluminescence film (Amersham Hyperfilm ECL ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then incubated for 75 min with the secondary antibody coupled to horseradish peroxidase (GE Healthcare). After four washing steps with TBST (5 min each) ...
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bioRxiv - Biophysics 2020Quote: ... Antibody oligomers were then separated with size exclusion chromatography (HiLoad Superdex 16/600 200 ps, GE Healthcare) and fractions were collected according to the SEC profile and SDS-PAGE (NuPAGE™ 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Cell Biology 2021Quote: ... The bound antibody was detected with ECL™ Western Blotting detection kit (GE Healthcare, Cat. No. RPN2209) on Hyperfilm ECL (Amersham GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: The visualization was performed after incubation with secondary antibodies coupled to HRP (GE Healthcare, NA931 and NA934) and Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... Sites of primary antibody binding were determined using an enhanced chemiluminescence system (GE Healthcare, Pittsburgh, PA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2020Quote: ... All membranes were incubated with secondary horseradish peroxidase conjugated antibody (1:10000; GE Healthcare, Chicago, Illinois, USA) for 1-2 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with secondary HRP-conjugated antibody (R&D) blots were developed using ECL reagent (GE Healthcare).
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... Membranes were washed in TBS-Tween and incubated with secondary HRP-conjugated antibody (GE Healthcare, Chicago, Illinois) at RT for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... The labelled antibodies were purified by passing through Disposable PD 10 Desalting Columns (GE Healthcare Life Sciences), concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore ...
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bioRxiv - Plant Biology 2019Quote: ... Immunodetection was carried out using specific antibodies and an ECL Plus Western Blotting Detection System (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... These were detected with appropriate secondary antibodies conjugated to HRP and visualized by ECL-Plus (GE Healthcare).
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then incubated for 75 min with the secondary antibody coupled to horseradish peroxidase (GE Healthcare). After four washing steps with TBST (5 min each) ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblots were washed with TBS-T and incubated with HRP-conjugated secondary antibodies (GE Healthcare, 1:5,000) for one hour in room temperature before development in films ...
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bioRxiv - Pathology 2020Quote: ... washed and incubated in secondary antibody before enhanced chemiluminescence detection using an ImageQuant LAS4000 imager (GE Healthcare). The primary antibodies used for WBs prepared using murine liver lysates were rabbit anti-LDLR (1:5,000 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated for two hours with horseradish peroxidase (HRP) conjugated secondary antibodies (GE Healthcare, Helsinki, Finland) at 1:6000.
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...