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Citations for GE Life Sciences :
251 - 300 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Cell Biology 2020Quote: Stathmin was purified using His-affinity (His-Trap HP, GE Healthcare) by first binding in binding buffer (20mM NaPO4 pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... This was expressed in E .coli strain NiCo21 (DE3) using conditions previously described(96) and purified by IMAC using NiHP (GE Healthcare Life Sciences) and AEC using QHP (GE Healthcare Life Sciences ...
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bioRxiv - Systems Biology 2020Quote: ... Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare) to remove free His-tagged GST and TEV as well as remaining impurities ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the protein without His6-tag was injected into a Superdex 75 10/300 GL (GE Healthcare) size exclusion column with the buffer B (50 mM NaH2PO4/Na2HPO4 pH 6.5 ...
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bioRxiv - Biophysics 2022Quote: ... The fusion protein was purified by fast protein liquid chromatography (FPLC) using a 5ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5M imidazole gradient (SKU I5513-100G ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were transferred to nitrocellulose membranes (GE Healthcare, 10600007). Antibodies used for western blots are listed in Supplementary Table 1 ...
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bioRxiv - Genetics 2021Quote: ... Proteins were transferred to Hybond-P PVDF membranes (GE Healthcare) in 1 × NuPAGE Transfer Buffer (Invitrogen by ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred on a PVDF membrane (GE Healthcare, 10600021) via wet electroblotting at 100 V in transfer buffer (20% methanol ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were electrophoretically transfected onto nitrocellulose membranes (GE Healthcare). Membranes were blocked in blocking buffer (Li-COR Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred onto nitrocellulose membranes (GE Healthcare Amersham) which were subsequently blocked in 5% milk PBS for 1 h at room temperature before being probed with relevant antibodies O/N at 4°C in 5% milk PBS ...
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bioRxiv - Genomics 2020Quote: ... Resolved proteins were transferred to a nitrocellulose membrane (GE Healthcare) and immunoblotted with the following antibodies at a 1/1,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to nitrocellulose membrane (GE Healthcare Life Sciences) using a semidry Trans-Blot transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were then transferred on the PVDF membrane (GE Healthcare) followed by membrane blocking with 5% non-fat dry milk in tris-buffered saline ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was transferred on a nitrocellulose membrane (GE Healthcare). A stripe of the membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to Nitrocellulose membrane (Hybond-C, GE healthcare) in transfer buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to Nitrocellulose membrane (Hybond-C, GE healthcare) in transfer buffer using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare, Chicago, US) and detection was performed using Cy-5 labelled anti-VHH antibody (Cy™5 AffiniPure Goat Anti-Alpaca IgG ...
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bioRxiv - Neuroscience 2022Quote: ... and protein transferred to a polyvinylidene difluoride membrane (GE Healthcare). After blocking (0.5 % BSA and 0.05% TWEEN-20 in Tris-buffered saline ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred to a nitrocellulose membrane (GE Healthcare), which was blocked with 5% dried milk in PBS-Tx (PBS ...
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bioRxiv - Immunology 2020Quote: ... Proteins were transferred to Hybond-ECL nitrocellulose membranes (GE Healthcare) for 2 h at 100 V and blocked with 1% Bovine Serum Albumine (BSA)-0.05% Tween-20 for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were blotted on nitrocellulose membranes (GE Healthcare Europe Gmb) and hybridized with the following antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the proteins transferred to nitrocellulose membranes (GE Healthcare, Amersham). After 1 h of incubation in blocking buffer [100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare, 45-0040002) using a Trans-Blot SD semi-dry transfer cell (Bio Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (GE Healthcare) with the Mini Trans-Blot® Cell (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were subsequently electrophoretically transferred onto nitrocellulose membranes (GE Healthcare) in a Trans-Blot SD Semidry Transfer Cell (BioRad ...
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bioRxiv - Immunology 2020Quote: ... Size-separated proteins were transferred to PVDF membrane (GE Healthcare) by semidry transfer ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were transferred to polyvinylidene fluoride membrane (GE Healthcare). The signals were detected using the Odyssey infrared imaging system (Odyssey Sa ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins were transferred to a NC (nitrocellulose) membrane (GE Healthcare). For the dot-immunoblot analysis ...
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bioRxiv - Biochemistry 2022Quote: ... Separated protein was then transferred onto nitrocellulose membrane (GE healthcare) or ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were transferred to a nitrocellulose membrane (GE Healthcare, #10600002). The membranes were blocked in a standard TBS-T buffer with 5% non-fat dry milk for one hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). Strep-tagged proteins were detected using rabbit anti-Strep-tag II antibody (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Separated proteins were transferred to nitrocellulose membranes (GE healthcare, UK) and probed with rabbit anti-c-Src primary antibodies raised against c-Src in its non-phosphorylated (1:2,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... After protein transfer onto 0.45 μM nitrocellulose membrane (GE Healthcare), the primary antibody mouse anti-HA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to 0.45-µm nitrocellulose membrane (GE Healthcare) for 2 h at 100V ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were electroblotted to PVDF membrane (0.2 μM, GE Healthcare) for 1 h at 100 V in ice-cold Bolt™Transfer Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred to nitrocellulose membranes (GE Healthcare). For immunoprecipitations ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare) were incubated with 1 ml of HeLa cell extract for 2 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
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bioRxiv - Genetics 2022Quote: ... and proteins were transferred onto a membrane (Nitrocellulose Blotting Membrane 10600002; GE Healthcare Life Sciences; PA; USA). Primary antibodies were incubated overnight at 4°C and included mouse anti-3OH-K (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant 6xHis-tagged mouse GRP78 was expressed in E coli BL21 cells and purified on a HisTrap™ HP Nickel column (GE healthcare, Uppsala, Sweden). Twenty micrograms (0.3 nmol ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 recombinant proteins were purified over a His-TRAP FF column (GE Healthcare) with 1-mL bed volume ...