Labshake search
Citations for Quantifoil Micro Tools :
401 - 450 of 618 citations for tert Butyl 2 chloro 5H pyrrolo 3 4 b pyridine 6 7H carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... were incubated for 3 min at room temperature and then loaded onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3). The grids were washed using the buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of samples were applied onto a glow-discharged gold grids covered with holey carbon film (Quantifoil, 300 mesh 1.2/1.3) and blotted using a Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biochemistry 2022Quote: A 3 µL of the protein solutions was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... 3.5 uL of sample was applied at a concentration of 3-5 mg/mL to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GLP-1R–Gs–Nb35 complex (3 μL at about 20 mg/mL) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3) and blotted subsequently ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of the freshly prepared mixture of the soluble S trimer and monomeric ACE2 (1:3 molar ratio) at ~1 mg/ml was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH, Germany), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Molecular Biology 2023Quote: 3 µl of freshly purified NbE-µOR-Fab module complex were applied onto holey gold grids (Quantifoil UltrAuFoil R1.2/1.3, 300 mesh), front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of Fcμ–J–CD5L was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh), blotted with filter paper in 100% humidity at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules (4 µl drop of 40 µM dilution) were placed onto a glow-discharged holey carbon grid (R2/1; Quantifoil) prepared on a JEC-3000FC (JEOL) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 4 µL of cell suspension (1500–2000 cells/µL) were applied to holey carbon R2/1 or R1/4 copper TEM grids (Quantifoil), and cells were plunge frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 3.6 μl were applied to glow-discharged gold holey carbon 2/1 300-mesh grids (Quantifoil). Grids were blotted for 4 s at 0 force and 1-s wait time before being vitrified in liquid propane using a Mark IV Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto plasma-cleaned Quantifoil R 2/1 (Cu, 400-mesh; Quantifoil Micro Tools GmbH) grids ...
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bioRxiv - Microbiology 2021Quote: ... 3μl was added to a glow-discharged R2/2 200 mesh holey carbon EM grid (Quantifoil) for 30 seconds and the sample was blotted for 1 second ...
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bioRxiv - Immunology 2021Quote: Quantifoil finder grids (R2/1 or R2/2, Au 200 mesh grid, Quantifoil Micro Tools, Germany) were glow discharged with a 30 mA current for 30 s using Edwards Sputter Coater (S150B) ...
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bioRxiv - Cell Biology 2022Quote: ... A volume of 2.5 μL was applied on glow-discharged UltrAuFoil Au200 R2/2 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2023Quote: The final high resolution data set was collected across 2 grids (Quantifoil 1.2/1.3 200 mesh) prepared with 1.8 mg mL-1 trimeric complex and 0.5 and 0.1 X CMC CHAPS ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh copper grids with a 2 nm continuous carbon layer (Quantifoil) were plasma-treated in a Pelco easiGlow glow discharging system (Ted Pella ...
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bioRxiv - Biophysics 2024Quote: ... Vero cells were seeded either on gold finder grids (200 mesh, R2/2, Quantifoil, Jena, Germany) or gold grids coated with lacey carbon film (XXBR Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... For the 20S:AKIRIN2:IPO9 sample R3.5/1 200 mesh Cu prefloated with 2 nm Carbon (Quantifoil) were used for plunge freezing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Three-hundred mesh R1.2/1.3 UltrAuFoil grids with a layer of 2 nm amorphous carbon (Quantifoil) were glow discharged in a Pelco easiGlow for 12 seconds under a 0.37 mBar vacuum with 25 mA current ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL of the purified MC1R-Gs complex was applied to a glow-discharged holey carbon EM grid (Quantifoil, Au 300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biochemistry 2020Quote: ... aliquots (3 μL) of the purified protein concentrated to ~ 10 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). For KCC3-DIOA samples ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of diluted sample was applied to a glow-discharged (R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... Peak fractions were collected and 3 μl of TSEN/pre-tRNA complex were applied onto freshly glow-discharged copper grids (Quantifoil, Cu R1.2/1.3) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Molecular Biology 2023Quote: ... A 2.5-3 μL aliquot of protein sample was dropped onto the glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: Three µl of purified PSI at a concentration of 3 mg/ml was applied to the glow-discharged holey carbon grids (Cu QUANTIFOIL® R1.2/1.3), then vitrified using a Leica GP2 plunge freezer (2.5 s blot ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μl of the sample was applied to glow discharged holey carbon-coated 200 mesh copper EM grids (Quantifoil Micro Tools), vitrified as above at 4°C and 100 % humidity.
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...
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bioRxiv - Immunology 2024Quote: ... a 4 μL aliquot of protein complex was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300-mesh) or graphene oxide (GO)-coated holey-carbon gold grids (EMR ...
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bioRxiv - Biophysics 2021Quote: ... A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion) and Ebola VLPs was applied with a volume of 3 µl on a glow discharged EM grid (Quantifoil, 200 Mesh, R2/1). The grid was blotted using No.1 Whatman paper for 3 sec from the back-side in a chamber with controlled humidity set to 70% and the temperature set to 24°C.
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bioRxiv - Biochemistry 2020Quote: An aliquot of 3 μL of SAM complex sample was applied to freshly glow-discharged holey carbon grid (Quantifoil R1.2/1.3, copper, 300 mesh). The grids were blotted for 6 s and plunge-frozen in liquid nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV plunger ...
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bioRxiv - Neuroscience 2022Quote: Extracted filaments were centrifuged at 3,000 g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 3,000 g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3 μL of purified CB1-Gi complex at 12 mg/ml to glow-discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh). The grids were blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Neuroscience 2022Quote: Extracted filaments were centrifuged at 3,000g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...