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Citations for Quantifoil Micro Tools :
251 - 300 of 509 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... For plunge freezing the cells were pipetted (4 µl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied onto freshly glow-discharged 200 square mesh Ultrafoil R2/2 gold grids (Quantifoil). The samples were plunge-frozen with a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Freshly glow-discharged finder gold quantifoil grids (200 mesh R2/2 – Quantifoil MicroTools GmbH, Germany) were sterilized in 70% EtOH and placed in a glass-bottomed MatTek dish (MatTek Corporation ...
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bioRxiv - Microbiology 2021Quote: ... were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil). The grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Biochemistry 2022Quote: ... Before spotting onto R2/1 grids with an extra 2 nm carbon layer (Quantifoil, Inc), the complex was diluted to a final concentration of ∼100 nM and a 5-fold excess of Met-tRNAiMet ...
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bioRxiv - Biochemistry 2021Quote: ... Cryo-EM grids were prepared using glow discharged R2/2 Cu 200 mesh grids (Quantifoil) pre-floated onto custom-made 2.9 nm continuous carbon film ...
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bioRxiv - Neuroscience 2022Quote: Holey copper or molybdenum EM grids (Quantifoil R 2/1 200 mesh; Quantifoil, Jena, Germany) were glow discharged (Harrick Plasma cleaner PDC-3XG ...
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bioRxiv - Microbiology 2023Quote: ... sample aliquots of 3 µL were applied onto UltrAuFoil R2/2 grids (300 mesh, Quantifoil). Grids were blotted for 1 s at 10°C with 100% relative humidity and immediately plunge frozen into liquid ethane using a Vitrobot Mark IV system (TFS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples (3 μl) were applied to Quantifoil 2/1 Cu 300 holey-carbon grids (Quantifoil) glow-discharged 60 s using a PELCO glow-discharge system (Ted Pella) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL) were applied to precoated (2 nm) R1.2/1.3 carbon-supported copper grids (Quantifoil), blotted for 4-5 s at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples (3 μl) were applied to 2/1 Quantifoil Cu 300 holey-carbon grids (Quantifoil); glow-discharged 60 s using a PELCO glow-discharge system (Ted Pella)]) ...
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bioRxiv - Biophysics 2024Quote: ... and 3 µL were applied to glow-discharged R2/2 holey carbon copper grids (Quantifoil). These grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 µL were applied to freshly glow-discharged R 2/1 holey carbon grids (Quantifoil) at 4 °C and 95% humidity ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto glow discharged cryo-EM grids (Quantifoil 2/1, 200 mesh, Au) overnight ...
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bioRxiv - Biophysics 2021Quote: ... 2.8 ul of the mixture was applied onto glow discharged (5 mA, 20 sec) R1.2/1.3 holey carbon gold grids (300 mesh, Quantifoil) at 22°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: Four microliters of purified TilA-TslA-TlaA1-TlaA2 complex at 0.5 mg ml-1 in Buffer B (Supplementary Table 5) was applied onto glow-discharged (60 s, 30 mA) 300 mesh R1.2/1.3 Au grids (Quantifoil). Grids were blotted for 3 s with blot force of +15 at 10°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... 2.2µl of the purified mixture at a concentration of 0.5 mg/mL was applied to glow discharged (30 s at 25mA) grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3.5 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Biochemistry 2023Quote: ... The most concentrated fraction was spun for 5 min at 13K rpm before applying 3 µL to UltraAuFoil R1.2/1.3 gold support grids (Quantifoil). Prior to sample applications grids were glow-discharged for 3 min in Pelco easiGlow glow-discharger (Pelco ...
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bioRxiv - Biochemistry 2023Quote: A 3.5 μL concentrated SPARTA sample was loaded onto a glow discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil) using a FEI Vitrobot at 4°C and 100% humidity ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were shaken at 1,200 rpm for 5 mins at 30°C using a plate shaker (Quantifoil Instruments) and absorption of 405 nm light measured using a FlexStation (Molecular Devices).
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bioRxiv - Biophysics 2024Quote: ... 10 mM DTT was incubated for 3 days at 30 °C and 2.5 μL of the solution was applied onto a Quantifoil R 1.2/1.3 200 mesh Cu grid (Quantifoil) and plunge-frozen in liquid ethane by a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of Fcμ–J–CD5L was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh), blotted with filter paper in 100% humidity at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules (4 µl drop of 40 µM dilution) were placed onto a glow-discharged holey carbon grid (R2/1; Quantifoil) prepared on a JEC-3000FC (JEOL) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 4 µL of cell suspension (1500–2000 cells/µL) were applied to holey carbon R2/1 or R1/4 copper TEM grids (Quantifoil), and cells were plunge frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 3.6 μl were applied to glow-discharged gold holey carbon 2/1 300-mesh grids (Quantifoil). Grids were blotted for 4 s at 0 force and 1-s wait time before being vitrified in liquid propane using a Mark IV Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto plasma-cleaned Quantifoil R 2/1 (Cu, 400-mesh; Quantifoil Micro Tools GmbH) grids ...
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bioRxiv - Microbiology 2021Quote: ... 3μl was added to a glow-discharged R2/2 200 mesh holey carbon EM grid (Quantifoil) for 30 seconds and the sample was blotted for 1 second ...
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bioRxiv - Immunology 2021Quote: Quantifoil finder grids (R2/1 or R2/2, Au 200 mesh grid, Quantifoil Micro Tools, Germany) were glow discharged with a 30 mA current for 30 s using Edwards Sputter Coater (S150B) ...
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bioRxiv - Cell Biology 2022Quote: ... A volume of 2.5 μL was applied on glow-discharged UltrAuFoil Au200 R2/2 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 3 µL of purified PPSA was applied to glow-discharged holy grids (Quantifoil, Cu200, R2/2) for 30s ...
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bioRxiv - Biochemistry 2023Quote: The final high resolution data set was collected across 2 grids (Quantifoil 1.2/1.3 200 mesh) prepared with 1.8 mg mL-1 trimeric complex and 0.5 and 0.1 X CMC CHAPS ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh copper grids with a 2 nm continuous carbon layer (Quantifoil) were plasma-treated in a Pelco easiGlow glow discharging system (Ted Pella ...
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bioRxiv - Biophysics 2024Quote: ... Vero cells were seeded either on gold finder grids (200 mesh, R2/2, Quantifoil, Jena, Germany) or gold grids coated with lacey carbon film (XXBR Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... For the 20S:AKIRIN2:IPO9 sample R3.5/1 200 mesh Cu prefloated with 2 nm Carbon (Quantifoil) were used for plunge freezing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Three-hundred mesh R1.2/1.3 UltrAuFoil grids with a layer of 2 nm amorphous carbon (Quantifoil) were glow discharged in a Pelco easiGlow for 12 seconds under a 0.37 mBar vacuum with 25 mA current ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of the cells were applied to a glow-discharged 200 mesh holey carbon grid copper grid (Quantifoil R1.2/3) and vitrified in a liquid ethane on a Vitrobot Mark IV (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μl of the sample was applied to glow discharged holey carbon-coated 200 mesh copper EM grids (Quantifoil Micro Tools), vitrified as above at 4°C and 100 % humidity.
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...