Labshake search
Citations for Quantifoil Micro Tools :
201 - 216 of 216 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Freshly purified ZorAB sample was concentrated to 2-3 mg/mL and 2.7 μL protein was applied onto the glow-discharged (30 s, 5 mA) grids (Quantifoil R0.6/1 300 mesh Au) and plunge-frozen into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were prepared by depositing 3 μl of the crystalline slurry onto a glow-discharged EM grid (Quantifoil, Cu 200 mesh, R2/2 holey carbon). Excess liquid was blotted away and the sample was vitrified using a Leica GP2 vitrification robot ...
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bioRxiv - Biochemistry 2022Quote: ... Several serial dilutions were prepared and 3 µl of each sample was loaded on Quantifoil R2/2Cu/Rh 300 holey-carbon-supported grids (Quantifoil Micro Tools GmbH, Jena, Germany). Initial cryo-EM sample preparations showed clear protein aggregation ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of sample (at the native 1 nM concentration) on glow discharged holey gold grids (Quantifoil Cu 1.2/1.3 400 mesh). Excess sample was removed by blotting with filter paper for 4 sec prior to plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of that mixture was transferred to a 3 mm freshly glow-discharged Quantifoil R2/2 Cu 300 mesh holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Excess liquid was blotted away using a Leica EM GP plunge-freezer at room temperature and 80% humidity (blot time 2-3 sec ...
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bioRxiv - Immunology 2020Quote: An aliquot of 3 μl purified protein of rNLRP1-rDPP9 or rNLRP1FIIND S969A-rDPP9 complex was applied to holey carbon grids (Quantifoil Au 1.2/1.3, 300 mesh), which were glow-discharged for 30 s at middle level in Harrick Plasma after 2 min evacuation ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was then resuspended in warm EM buffer and 3 μL of this sample was applied to a carbon on gold grid (Quantifoil R2/2 Au 200 mesh), recently glow-discharged for 1 min at 30 mA ...
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bioRxiv - Neuroscience 2024Quote: ... conventional methods were used: 3 ul of purified tau filaments were applied to glow-discharged holey carbon grids (Quantifoil Au R 1.2/1.3, 300 mesh) and mechanically blotted (3-4 seconds ...
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bioRxiv - Biophysics 2023Quote: 2.5μL aliquots of Fks1 at a concentration of about 3 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2023Quote: 3 µL of the human CDCA7264-371 in complex with the nucleosome harboring a single hemimethylated CpG in the 3’-linker DNA was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... respectively) and 3 μl were kept 5 min at RT until their application onto glow-discharged holey carbon grids (Quantifoil, Au 300 mesh, R 0.6/1). The grids were blotted and then plunged into liquid ethane using a FEI Vitrobot Mark IV at 20°C and 95% relative humidity ...
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bioRxiv - Biophysics 2024Quote: 3 μL of concentrated protein sample was applied to a glow-discharged (30 s at 15 mA) grid (Quantifoil Au or Cu R1.2/1.3 300 mesh) and after a wait time of 5 s ...
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bioRxiv - Biochemistry 2024Quote: 3µl of the AtGDH-AKG bound binary complex (3 mg/ml) was applied to freshly glow-discharged holey carbon grids (Quantifoil Au R 0.6/1.0 300 mesh). For the apo form AnGDH dataset ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 μl samples of GDN-purified proteins at a concentration of 1.5-3 mg ml−1 were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 or R0.6/1 Au 200 mesh). Excess liquid was removed in a controlled environment (4°C and 100% relative humidity ...
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bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...