Labshake search
Citations for Quantifoil Micro Tools :
151 - 200 of 216 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a volume of 6-8 μL of each sample was applied onto a gold grid covered by a holey gold film (UltrAuFoil 2/1, Quantifoil Micro Tools GmbH, Jena, Germany). Excess of liquid was blotted automatically between two strips of filter paper or only from the backside of the Grid ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: The purified PACAP27–VIP2R–Gs complex (3 μL at 3.7 mg per mL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: 3 µl of S-layer suspension were applied to glow-discharged 300 mesh copper Quantifoil grids (R2/2, Quantifoil, Jena, Germany), blotted for 3 – 5 seconds and rapidly injected into liquid ethane using a homemade plunge-freezer ...
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bioRxiv - Biochemistry 2020Quote: ... the grids were prepared by applying 3 μl sample on glow-discharged holey carbon grids (Quantifoil Mo 2/2 200 mesh). The sample was ice-embedded using a LEICA EM GP2 (Leica Microsystems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples (3 µL) were applied to a glow-discharged UltrAuFoil R1.2/1.3 Au 300 gold foil grids (Quantifoil GmbH, Großlöbichau, Germany) and were flash frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... samples were stored on ice and applied to the cryo-grids (2 nm precoated Quantifoil R3/3 holey carbon supported grids) at a concentration of 10 μM and vitrified using a Vitrobot Mark IV (FEI) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil, for samples without FFosC), or Cu 200 Holey Carbon R2/1 grids (Quantifoil ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample mixture was applied to a freshly glow-discharged copper R2/2 200 grid (Quantifoil Micro Tools), pre-blotted for 30 seconds ...
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bioRxiv - Biochemistry 2021Quote: The purified TIP39–PTH2R–Gs complex (3 μL at 8.5 mg per mL) was applied on a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The complex (3 μL) was quickly applied on a glow-discharged UltraAuFoil R1.2/1.3 300 mesh holey Au grids (Quantifoil Micro Tools GmbH), blotted with Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of the cells were applied to a glow-discharged 200 mesh holey carbon grid copper grid (Quantifoil R1.2/3) and vitrified in a liquid ethane on a Vitrobot Mark IV (Thermo Scientific ...
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bioRxiv - Biophysics 2024Quote: Approximately 3□μL of the sample solution were put on newly glow-discharged TEM grids (Quantifoil R2/1, Cu, mesh 200) and plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Cryo-EM grids were prepared by applying 3 µl of concentrated sample onto 400-mesh R1.2/1.3 UltrAuFoil grids (Quantifoil Micro Tools GmbH), which had rendered hydrophilic by glow discharging at 15mA for 60seconds with a PELCO EasyGlow device (TED PELLA ...
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bioRxiv - Biochemistry 2022Quote: ... stock solution was mixed with 3.5 µL of the KWOCA 4 sample and 3 µl were immediately loaded onto the UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample loading the grids were subjected to Ar/O2 plasma cleaning for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
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bioRxiv - Neuroscience 2023Quote: ... each time was sequentially blotted 3 times to 300-mesh holey carbon Au R1.2/1.3 grids treated with 25s of glow discharge (Quantifoil, Micro Tools GmbH). Fluorinated Fos-choline-8 (0.65 mM ...
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bioRxiv - Microbiology 2024Quote: ... The sample solution (3 μl) was applied to glow-discharged (20 mAmp, 30 sec, Quorum GloQube) Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH), blotted for 5 s using blot force 0 and plunge frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 3 min at room temperature and then loaded onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3). The grids were washed using the buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μL aliquots of the cell suspension were applied to glow-discharged R2/2 200 mesh copper Quantifoil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Immunology 2019Quote: 4 μL of C5-OmCI-RaCI-CirpT1 in PBS (0.3 mg/ml) was applied to freshly glow-discharged (20 s, 15 mA) carbon-coated 200-mesh Cu grids (Quantifoil, R1.2/1.3). Following incubation for 10 s ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of samples were applied onto a glow-discharged gold grids covered with holey carbon film (Quantifoil, 300 mesh 1.2/1.3) and blotted using a Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biochemistry 2022Quote: A 3 µL of the protein solutions was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... 3.5 uL of sample was applied at a concentration of 3-5 mg/mL to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GLP-1R–Gs–Nb35 complex (3 μL at about 20 mg/mL) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3) and blotted subsequently ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of the freshly prepared mixture of the soluble S trimer and monomeric ACE2 (1:3 molar ratio) at ~1 mg/ml was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH, Germany), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of the microtubule sample was deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (35 °C ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: 3 µl of freshly purified NbE-µOR-Fab module complex were applied onto holey gold grids (Quantifoil UltrAuFoil R1.2/1.3, 300 mesh), front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL of the purified MC1R-Gs complex was applied to a glow-discharged holey carbon EM grid (Quantifoil, Au 300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biochemistry 2020Quote: ... aliquots (3 μL) of the purified protein concentrated to ~ 10 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). For KCC3-DIOA samples ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of diluted sample was applied to a glow-discharged (R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Immunology 2022Quote: ... detergent at a final concentration of 0.005 mM was added to each sample and 3 µl of that solution was immediately loaded onto plasma-cleaned QuantiFoil R 2/1 (400-mesh; Quantifoil Micro Tools GmbH) and UltrAuFoil R 1.2/1.3 grids (300-mesh ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μl aliquots of purified mitoribsomes was incubated for 30 s on glow-discharged holey carbon grids (Quantifoil R2/2, 300 mesh, copper) pre-coated with a home-made continuous carbon film with thickness of ~ 27 Å ...
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bioRxiv - Immunology 2020Quote: ... and 3 µl was immediately loaded onto plasma cleaned Quantifoil R 2/1 holey carbon copper gri (Cu, 400-mesh, Quantifoil Micro Tools GmbH). Blotted grids were plunge-frozen into nitrogen-cooled liquid ethane.
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bioRxiv - Biophysics 2019Quote: ... A 3 μl aliquot of ribosomes at a concentration of 100 nM was placed on plasma-cleaned UltrAuFoil® grids (Quantifoil, R1.2/1.3) and vitrified using Vitrobot Mark IV (FEI company) ...
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bioRxiv - Biochemistry 2022Quote: ... Peak fractions were collected and 3 μl of TSEN/pre-tRNA complex were applied onto freshly glow-discharged copper grids (Quantifoil, Cu R1.2/1.3) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... 2–3 μL of sample solution was applied to a holey carbon grid (Quantifoil R2/1, Mo 300 mesh; Quantifoil Micro Tools GmbH) covered with a thin amorphous carbon film at 4 °C with 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Molecular Biology 2023Quote: ... A 2.5-3 μL aliquot of protein sample was dropped onto the glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: Three µl of purified PSI at a concentration of 3 mg/ml was applied to the glow-discharged holey carbon grids (Cu QUANTIFOIL® R1.2/1.3), then vitrified using a Leica GP2 plunge freezer (2.5 s blot ...
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bioRxiv - Biophysics 2021Quote: ... A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion) and Ebola VLPs was applied with a volume of 3 µl on a glow discharged EM grid (Quantifoil, 200 Mesh, R2/1). The grid was blotted using No.1 Whatman paper for 3 sec from the back-side in a chamber with controlled humidity set to 70% and the temperature set to 24°C.
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bioRxiv - Biophysics 2022Quote: ... an aliquot of 3 μL of sample was applied onto a glow-discharged holey carbon-coated copper grid (300 mesh, QUANTIFOIL® R 2/1) or holey gold grid (300 mesh ...
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bioRxiv - Biochemistry 2020Quote: An aliquot of 3 μL of SAM complex sample was applied to freshly glow-discharged holey carbon grid (Quantifoil R1.2/1.3, copper, 300 mesh). The grids were blotted for 6 s and plunge-frozen in liquid nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV plunger ...
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bioRxiv - Neuroscience 2022Quote: Extracted filaments were centrifuged at 3,000 g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 3,000 g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3 μL of purified CB1-Gi complex at 12 mg/ml to glow-discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh). The grids were blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Neuroscience 2022Quote: Extracted filaments were centrifuged at 3,000g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Biochemistry 2023Quote: ... The detergent was added shortly before applying 3 μL of UBR5Dimer∼UbD∼UBE2D2 to freshly glow-discharged holey carbon grids (Quantifoil, R1.2/1.3, 200 mesh). The sample was consequently plunge-frozen into liquid ethane at 95 % humidity and 4°C (blot force 3 ...