Labshake search
Citations for IDT DNA :
1 - 50 of 244 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... with DsiRNA (Integrative DNA Technologies, Coralville, IA; Cat# 51-01-14-03) used as a negative control ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Amplicons were then A-Tailed and ligated to xGen-UDI-UMI Adapters (Integrative DNA Technologies, Coralville, IA) using a KAPA HyperPrep PCR-Free kit (Roche ...
-
bioRxiv - Synthetic Biology 2024Quote: ... all primers were synthesized by Integrative DNA Technologies (Coralville, IA), and all PCR reactions were set up with Q5 Master Mix (New England Biolabs ...
-
bioRxiv - Immunology 2024Quote: ... we designed a gblock (IDT DNA, Coralville, CA) of 2,728bp sharing homology with DENV NS3 gene ...
-
bioRxiv - Microbiology 2024Quote: ... TaqMan probe and primers (IDT DNA) specific to the B ...
-
bioRxiv - Molecular Biology 2024Quote: ... Spliceosome proteins for fusion experiments were ordered as eBlocks from IDT DNA. An expression backbone originally containing wild-type Cas7-11 (pDF0506 ...
-
bioRxiv - Neuroscience 2024Quote: ... All primers were from IDT DNA Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: Cargo constructs were ordered as eBlock Gene Fragments from IDT DNA and cloned by Gibson Assembly ...
-
bioRxiv - Cell Biology 2024Quote: ... The codon-altered PfCAP-H gene blocks (GB70.2 and GB71, IDT DNA) were amplified with oJDD6598/6599 and oJDD6196/6197 ...
-
bioRxiv - Cell Biology 2024Quote: ... gene blocks were purchased from IDT DNA, and restriction enzymes were purchased from New England Biolabs ...
-
bioRxiv - Cell Biology 2024Quote: ... as previously described.10 Genetic correction of the pathological gene variant in the patient-derived iPSC line UMGi137-A clone 2 was performed using ribonucleoprotein-based CRISPR/Cas9 using crRNA/tracrRNA and Hifi SpCas9 (IDT DNA technologies) by targeting exon 15 of the LZTR1 gene ...
-
bioRxiv - Immunology 2024Quote: ... 65 pmol of sgRNA targeting mouse Pdl1 (IDT DNA) and 30 pmol of S.p ...
-
bioRxiv - Immunology 2024Quote: The sgRNA oligonucleotides (IDT DNA) targeting mouse Pvrl2 or Pvr were cloned into pSpCas9(BB)-2A-GFP plasmid (PX458 ...
-
bioRxiv - Immunology 2024Quote: ... Pvrig KO NK cells were isolated from spleens of Pvrig KO mice and transfected with two sgRNAs targeting mouse Tigit or Cd96 (IDT DNA) using Cas9-gRNA RNP-directed gene deletion on day 5 ...
-
bioRxiv - Immunology 2024Quote: ... the BaEVRless gene was codon-optimized and synthesized as a gBlock fragment from IDT DNA. The BaEVRless sequence was amplified from the gBlock by KAPA HiFi HotStart PCR kit using primer 1 and 2 ...
-
bioRxiv - Immunology 2024Quote: ... The CAR cassette was synthesized as a gBlock fragment from IDT DNA, amplified by primer 13 and 14 ...
-
bioRxiv - Cancer Biology 2024Quote: ... RhAMP SNP primers were designed by IDT DNA (assay name CD.GT.WVSM3699.1). RhAMP SNP assay was carried out per manufacturer’s protocol on a QuantStudio 12K Flex (Applied Biosystems ...
-
bioRxiv - Biochemistry 2024Quote: ... and G-quadruplex forming DNA and RNA oligonucleotides were purchased either from Integrative DNA Technology (IDT) or synthesized in-house as described below ...
-
bioRxiv - Biophysics 2024Quote: 601 DNA flanked by 20 bp of linker DNA (185 bp; 20-N-20) was amplified using polymerase chain reaction (PCR) using primers (IDT DNA) containing 20 bp overhangs ...
-
bioRxiv - Cancer Biology 2024Quote: ... Custom PAGE-purified single-strand oligonucleotides with a pattern of 20 random bases and surrounded by 25 nucleotides homologous to the vector insertion site were synthesized by IDT DNA Technologies (Supplementary Table 5) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... gBlocks encoding heavy and light chain frameworks were purchased from IDT DNA Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... These gRNA target sites were used to produce two Alt-R CRISPR-Cas9 crRNAs (IDT DNA, Coralville, IA) which were individually hybridized to a universal 67-mer Alt-R CRISPR-Cas9 tracrRNA (IDT DNA ...
-
bioRxiv - Neuroscience 2024Quote: ... which were individually hybridized to a universal 67-mer Alt-R CRISPR-Cas9 tracrRNA (IDT DNA) to produce two gRNAs ...
-
bioRxiv - Immunology 2024Quote: ... Ithaca NY)(58). CUMT8 E. coli was colonized with plasmid pSIM6 (S. Gottesman NIH/NCI) and electroporated with a geneBlock (IDT DNA) that contained the sequence of GP61-80 and a Kanamycin resistance gene targeted to the C-teminus/3’ UTR of the E ...
-
bioRxiv - Microbiology 2024Quote: ... NC1 (IDT DNA; 51-01-14-04), was used.
-
bioRxiv - Molecular Biology 2024Quote: ... parvum was synthesized de novo by IDT DNA with codon optimization for E ...
-
bioRxiv - Molecular Biology 2024Quote: ... Utilizing the Codon Optimization Tool (IDT DNA), the nucleotide sequence of the gene was changed for efficient expression in E ...
-
bioRxiv - Molecular Biology 2024Quote: ... Synthetic gBlocks (IDT DNA Technologies) were generated containing the entire ND7 pre-edited (PE ...
-
bioRxiv - Microbiology 2024Quote: ... from mRNA sequence (GenBank Accession # NM_001004343.3) with addition of EcoR1 and SalI restriction sites was synthesized by IDT DNA Technologies (Coralville ...
-
bioRxiv - Synthetic Biology 2024Quote: ... coli K12 (IDT DNA) and cloned in the non-replicating integration plasmid backbone pMTV210 by GenScript (Nanjing ...
-
bioRxiv - Neuroscience 2024Quote: ... Two 140 nt long PAGE purified Ultramer single stranded DNA Oligos (IDT DNA) with three phosphorotioate modifications on each end (Renaud et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... Primers were purchased from IDT DNA. Protein A-agarose (cat no ...
-
bioRxiv - Microbiology 2024Quote: ... Oligonucleotides and gBlocks were from IDT DNA, Coralville ...
-
bioRxiv - Cell Biology 2024Quote: ... Human FKBP12 (324 base pairs) was ordered as a gBlock (IDT DNA), eGFP was amplified from a previously published plasmid (Yu et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... The StrepII-TEV sequence (126 bp) was ordered as gBlock fragment from IDT DNA with overhangs for insertion into the pcDNA3 backbone and ligation to eGFP ...
-
bioRxiv - Plant Biology 2024Quote: ... All primers used in the PCR were synthesized from IDT DNA, USA (https://www.idtdna.com/ ...
-
bioRxiv - Neuroscience 2024Quote: For the generation of pPGK_CD9_mScarletI_Flag DNA coding for CD9_Flag was obtained as a gBlock from IDT DNA and cloned into a pcDNA3.1 vector containing a PGK promoter using restriction enzyme cloning ...
-
bioRxiv - Microbiology 2024Quote: ... A repair fragment of approximately 500 bases containing the 3-Myc-Tag inserted just after the start codon and containing homology regions on each side was synthesized (g-block IDT DNA company). This repair fragment was inserted into the pCR-Blunt-II-Topo vector (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... A repair fragment of approximately 500 bases containing the 3-Ty- Tag inserted just after the in frame of the sequence and containing homology regions on each side was synthesized (g-block IDT DNA company). This repair fragment was inserted into the pCR-Blunt-II-Topo vector (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... two gBLOCKS (IDT DNA, Coralville, IA) were designed such that there was 30 bp overlap on the 5’ end of the first gBLOCK (50_STOP_FS_gBLK_1 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... mRNA of the MGaptamer sequence only – specifically rArCrUrGrGrArUrCrCrCrGrArCrUrGrGrCrGrArGrArGrCrCrArGrGrUrArArCrG rArArUrGrGrArUrCrCrArArU – was purchased from IDT DNA. The lyophilized mRNA was re-suspended in nuclease-free water at its concentration was determined by a Nanodrop 2000c ...
-
bioRxiv - Biophysics 2024Quote: ... Amine-modified DNA strands for imager strands were purchased from IDT DNA. Cy3B-NHS Ester fluorophores (PA63101 ...
-
bioRxiv - Biophysics 2024Quote: The Widom 601 DNA flanked by 19 bp of linker DNA at each end (185 bp) was amplified using polymerase chain reaction (PCR) using primers (IDT DNA) containing 19 bp overhanging regions using the linear double-stranded 147 bp 601 DNA as a template ...
-
bioRxiv - Biochemistry 2024Quote: ... nanoLuc (gBlock, IDT DNA) was subcloned into pENTR1a by flanking XbaI/XhoI restriction sites ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Primers were purchased from Eurofins Genomics (for 50 bp or 80 bp homology arms) or from IDT DNA (for 155 bp homology arms). PCR reactions were set up in 100 µL as follows using Q5 high fidelity DNA polymerase (New England Biolabs M0491S ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... both purchased from Integrated DNA Technologies (IDT DNA, Coralville, IA, USA). crRNAs were designed on the EuPaGDT platform http://grna.ctegd.uga.edu/ (Peng and Tarleton ...
-
bioRxiv - Molecular Biology 2024Quote: ... Gene expression was measured using PCR (all primers obtained from IDT DNA Technologies, Coralville IL) with β-actin as reference normalization gene ...
-
bioRxiv - Developmental Biology 2024Quote: ... ordered at the 2 nmol scales as Custom DsiRNAs with standard purification (IDT DNA Technologies), resuspended at 100 μM in 1x Bombyx injection buffer (pH 7.2 ...
-
bioRxiv - Bioengineering 2024Quote: The primers used for mutations were synthesized by IDT DNA. BL21(DE3 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... ordered as Custom DsiRNAs with standard purification (IDT DNA Technologies), resuspended at 100 μM in 1X Bombyx injection buffer (pH 7.2 ...