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Citations for SouthernBiotech :
1301 - 1328 of 1328 citations for Goat Anti Rabbit IgG HRP H+L since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
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bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... formaldehyde fixed parasites were adhered to glass slides and blocked with 50% FCS in PBS for 45 min before staining with primary anti-VSG (1:10,000) and secondary Alexa Fluor (1:1,000) antibodies and mounting with DAPI Fluoromount-G® (Southern Biotech). Imaging was performed with an Axioscope 2 widefield fluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... was used except that plates were coated with anti-human kappa and lambda light chain specific mouse antibodies (Southern Biotech) at 1:1 ratio diluted to 1 μg/ml in PBS overnight ...
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bioRxiv - Microbiology 2024Quote: ... bound S8V1-157 was detected by incubating the microspheres with 2 μg/ml PE-conjugated rat anti-mouse IgG1 (SB77e, SouthernBiotech) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... A baseline of 30 s was recorded before stimulation with 1 µg/ml anti-λ antibody (Southern Biotech, Birmingham, AL, USA). Flow cytometry standard files were analyzed using FlowjoTM software for Windows ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in calcium-containing HBSS and incubated at room temperature for 5 minutes before activation by anti-IgM F(ab′)2 (Southern Biotech) or VLPs ...
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bioRxiv - Biochemistry 2020Quote: ... which was produced by the conjugation reaction between anti-fluorescein antibody (600101096; Rockland Immunochemicals, PA or 6400-01; Southern Biotech, AL) and NHS-activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were incubated with a Goat F(ab’)2 anti-human Kappa-Alexa Fluor 647 (pre-diluted in PBS 0.1%BSA; 2.5 μg/mL, Southern Biotech #2062-31) to detect bound Fab fragments ...
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bioRxiv - Systems Biology 2022Quote: ... for capture and biotinylated anti-human insulin antibody (Eli Lilly and Company) with horseradish peroxidase conjugated streptavidin (7100-05, Southern Biotech) for detection ...
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bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS/0.1% Tween 20 and wells were incubated with alkaline phosphatase-conjugated anti-human Fc (Southern Biotech; P7998) followed by 2 hour incubation ...
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bioRxiv - Immunology 2021Quote: ... buffer (PBS with 0.1% bovine serum albumin and 0.1% sodium azide) containing the PE-labeled tetrameric pBF2*1501 complex and fluorescein isothiocyanate (FITC)-labeled anti-CD8 monoclonal antibody (SouthernBiotech, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
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bioRxiv - Immunology 2023Quote: ... The concentration of Ig in serum supernatants was measured using a standard sandwich ELISA with anti-human Ig primary (Cat#: 2048-01, Southern Biotech) and secondary antibodies (Cat# ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 1×107 cells were starved for 30 min at 37°C in 1 ml FCS-free RPMI and then treated with 10 µg/ml anti-λ antibody (Southern Biotech) under shaking at 750 rpm for the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... Beads were prepared as described for Eα peptide presentation and coated with different ratios of biotinylated anti-IgM (Southern Biotech, #1021-08) and biotinylated ovalbumin (OVA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with PBS-BSA and stained with secondary antibodies (5 μM Alexa Fluor® 488 anti-mouse (SouthernBiotech; 1031-30) and 10 μM Alexa Fluor® 647 anti-human (SouthernBiotech ...
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bioRxiv - Immunology 2020Quote: ... followed by two rounds of fluorescence-activated cell sorting (FACS) of cells stained with FITC-Conjugated rat anti-mouse IgG1 (Southern Biotech 1144-02), and rounds of soft-agar cloning in SeaPlaque agarose ...
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bioRxiv - Immunology 2023Quote: ... primary B cells (107/ml) were stained for 5 min on ice with 10 μg/ml of biotinylated anti-IgM (Southern Biotech, 1021-08) and washed with Imaging Buffer ...
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bioRxiv - Immunology 2023Quote: ... sonicated to disrupt bead:bead aggregates (Bioruptor®: HIGH, 5 cycles, 30” on/off) and coated with different ratios of biotinylated anti-IgM (Southern Biotech, #1021-08) and biotinylated Eα52-68 peptide (Biotin-GSGFAKFASFEAQGALANIAVDKA-COOH ...
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bioRxiv - Cancer Biology 2020Quote: Primary CLL samples were treated with 20 µg/ml anti-IgM F(ab’)2 or control F(ab’)2 (both Southern Biotech, Cambridge Biosciences, UK) for 5 minutes at 37°C/5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the cells were stained with DAPI solution and washed three times before mounting with Fluoromount-G® Anti-Fade Mounting Medium (Southern Biotech, Birmingham, AL). Images were captured using Zeiss Observer Z1 confocal microscope (Carl Zeiss ...
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bioRxiv - Immunology 2022Quote: ... Anti-BMP6 and isotype control were injected at a dose of 10mg/kg body weight diluted in sterile low endotoxin PBS and injected intraperitoneally into mice.G-CSF complexes were prepared as described in (Rubinstein et al., 2013) using human G-CSF (Filgrastim) and anti-human G-CSF (Clone BVD11-37G10, Southern biotech, Cat. No. 10128-0). 7.5μg of anti-G-CSF and 1.5μg of G-CSF were complexed ...