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Citations for SouthernBiotech :
951 - 1000 of 1272 citations for Goat Anti Rabbit IgG HRP H+L since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:500). After washing ...
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bioRxiv - Cell Biology 2023Quote: ... as well as FITC- and Texas Red-conjugated goat polyclonal anti-human lambda-light chain (cat. 2070-02 and 2070-07) were obtained from Southern Biotech and were used for immunofluorescent microscopy ...
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bioRxiv - Neuroscience 2024Quote: ... mounted (Vector Laboratories, H-1400-10 or Fluoromount-G, SouthernBiotech), and stored at 4° C until imaging.
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bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), or sheep anti-mouse immunoglobulin (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech; 4010-05; 1:15000), sheep anti-mouse immunoglobulin (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells were washed with ice cold PBS buffer and then stained with a PE-labelled anti-mouse IgG secondary antibody (Southern Biotech, Birmingham, AL) at 1 μg/ml concentration for 30 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 30 minutes with the following secondary antibodies diluted in PBS 2.5% BSA: donkey anti-goat Alexa Fluor 488 1:200 (Southern Biotech 3425-30) and donkey anti-rabbit Alexa Fluor 555 1:200 (Thermo fisher scientific A32794) ...
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bioRxiv - Microbiology 2022Quote: ... the beads were incubated with 1 µg/mL Alexa Fluor647-conjugated goat F(ab’)2 anti-human kappa (Southern Biotech, 2062-31) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in fresh dilution solution with secondary antibody (goat anti-mouse IgG2a AlexaFluor 488, 1:250, Southern Biotech, cat# 1080-30) overnight at 4°C with gentle shaking ...
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bioRxiv - Immunology 2019Quote: ... 4 × 106 primary B cells in 0.1 ml of imaging medium were stimulated with 20 µg/ml soluble goat anti-Igκ antibodies (Southern Biotech, #1050-01). Cells were lysed in RIPA buffer (30 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were washed in phosphate buffered saline containing 0.05% Tween 20 (PBST) and incubated with 10% rabbitserum and then goat anti-type V collagen antibody (Southern Biotech, Birmingham, AL) at 1:1000 for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Microbiology 2021Quote: ... and for sMAC with 1:5,000 HRP-conjugated streptavidin (Southern Biotech). Finally ...
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bioRxiv - Immunology 2023Quote: ... followed by an incubation of 1:5000 streptavidin-HRP (Southern Biotech). To measure deposition of C3b on the IgM coated plates ...
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bioRxiv - Immunology 2022Quote: ... plus HRP-conjugated streptavidin (1:7000, Southern Biotech, Cat# 7100-05) were used for measuring mouse IgA antibody ...
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bioRxiv - Bioengineering 2023Quote: ... followed by the HRP-conjugated secondary antibody (Southern Biotech 4050-05) at 1:10,000 in 2% BSA in PBS-T and detection.
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bioRxiv - Genetics 2020Quote: ... washed with PBT and then incubated with Alexa-488 anti-Rabbit secondary antibody (#6441-30 SouthernBiotech, 1:250) for 2h ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit anti-rat conjugated to horseradish peroxidase was used as a secondary Ab (1:1000; Southern Biotech) and incubated with TBST (5% BSA ...
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bioRxiv - Immunology 2020Quote: ... IgG and/or IgM (Southern Biotech; Birmingham, AL, USA), was added at 3 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... IgG and/or IgM (Southern Biotech; Birmingham, AL, USA). After 30 minutes of incubation at 800 rpm in the dark ...
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bioRxiv - Synthetic Biology 2023Quote: ... an IgG isotype control (Southern Biotech Catalog# 0109-30) to correct the MFI of AF488 for non-specific staining.
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bioRxiv - Immunology 2022Quote: ... followed by incubation with streptavidin-HRP (Sigma-Aldrich, Invitrogen and Southern Biotech), and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated antibodies were subsequently probed with streptavidin-HRP (Southern Biotech, 7100-05). Detection antibody was removed ...
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bioRxiv - Immunology 2021Quote: ... 1:300 for IgA.] HRP conjugated antibodies were all from Southern BioTech: IgM (#1021-05) ...
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bioRxiv - Immunology 2023Quote: ... and IgA were determined by using the Clonotyping System-HRP (Southern Biotech) with the Mouse Ig Isotype Panel (Southern Biotech ...
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bioRxiv - Neuroscience 2021Quote: ... for an additional 1 h and mounted in Fluoromount-G (Southern Biotech). For mice implanted with wire bundles ...
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bioRxiv - Immunology 2021Quote: ... standards (IgM and IgG from Southern Biotech; IgE from Biolegend) and samples were applied to the coated plate for 1 hr at room temperature (RT) ...
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bioRxiv - Immunology 2020Quote: ... Horse spleen ferritin-specific IgA levels in the supernatant of the faecal homogenates was determined by enzyme-linked immunosorbent assay in plates coated with horse spleen ferritin followed by detection with horseradish peroxidase-conjugated goat anti-mouse IgA (Southern Biotech, Birmingham, AL, USA) using optEIA (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was then resuspended in 100 µL ice-cold PBS containing 4 µg of FITC-coupled goat anti-mouse IgA antibody (SouthernBiotech, Imtec Diagnostic, #1040-02). The ratio of 4 µg of the respective antibody to stain 109 bacteria was previously evaluated to be the maximum amount of antibody that can be used without resulting in unspecific staining of non-IgA coated bacteria by using fecal samples from Rag1−/− mice as non IgA-coated negative controls ...
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bioRxiv - Genetics 2019Quote: ... washed and incubated with HRP-conjugated secondary antibody (1:10 000, Southern Biotech). Immunoblots were developed using ECL prime (GE healthcare ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and incubated with 100 μl HRP-conjugated streptavidin (Southern Biotech) at a dilution of 1:1000 for 1 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Chlamydia-specific Abs were detected using HRP-based SBA Clonotyping System (Southern Biotech).
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bioRxiv - Immunology 2019Quote: ... A secondary polyclonal goat antibody (Southern Biotech, Birmingham, AL) directed to the human kappa light chain and conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2020Quote: ... 1:250 ColIV (polyclonal goat, 1340-01, Southern Biotech), 1:250 CD31 (monoclonal mouse ...
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bioRxiv - Immunology 2023Quote: ... After blocking with 10% goat serum (SouthernBiotech; Birmingham, AL), samples were incubated with mouse anti-PI(3,4)P2 mAB or mouse anti-PI(3,4,5)P3 mAB conjugated with FITC for 1 hour at 37°C [24] ...
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bioRxiv - Bioengineering 2023Quote: ... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1% BSA in PBS twice before incubating with secondary anti-rat IgG antibody conjugated with APC (1:500; Southern Biotech, AL, USA, Catalog no. 3010-11L). Cells were analyzed in BD FACSVerse™ flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: Antigen-specific IgG (Cat. No. 1015-05, Southern Biotech, AL, USA), IgA (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100μL IgG-biotin HU adsorbed (Southern Biotech) for 2hr at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and blocked with unlabeled mouse IgG (Southern Biotech) prior to subsequent staining and analysis.
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bioRxiv - Immunology 2020Quote: ... Total serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Cell Biology 2022Quote: ... The HRP–conjugated secondary antibodies were purchased from SouthernBiotech (mouse, WB 1:20 000) and Protein A HRP–conjugated were purchased from Invitrogen (WB 1 ...
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bioRxiv - Bioengineering 2023Quote: ... The slices were blocked in 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature and then incubated with a primary rabbit anti-RLuc antibody (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 h at RT ...
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bioRxiv - Immunology 2022Quote: ... Standard curves were generated using unlabeled mouse IgG (Southern Biotech #0107-01), and detection was performed with anti-mouse IgG-HRP (Southern Biotech) ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were incubated with 100 µL IgG-biotin HU adsorbed (Southern Biotech) for 2 hr at RT ...
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bioRxiv - Immunology 2022Quote: ... Class-switched serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Microbiology 2023Quote: ... and mAb isotypes were determined using SBA Clonotyping System-HRP (Southern Biotech, Birmingham, AL, USA), according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... then incubated for 2 h at room temperature with Streptavidin-alkaline phosphatase from Southern Biotech at 2 μg/mL ...