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Citations for SouthernBiotech :
1051 - 1100 of 1328 citations for Goat Anti Rabbit IgG HRP H+L since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the membrane was incubated with a 1: 2500 dilution of streptavidin-HRP (Southern Biotech; 7100-05) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed and developed with streptavidin-HRP antibody (1:1000; SouthernBiotech, Birmingham, AL, USA) and 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by quantification of IgG1 abundance using the SBA Clonotyping System-HRP kit (SouthernBiotech; 5300-05) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature and washed and mounted with Fluoromount-G (SouthernBiotech, #0100-01). For dendritic spine analysis ...
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bioRxiv - Immunology 2023Quote: ... bound antibodies were detected using peroxidase conjugated goat antibodies to IgA (SouthernBiotech), IgG1 (SouthernBiotech) ...
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bioRxiv - Immunology 2022Quote: ... IgG was detected using phycoerythrin-conjugated mouse antibodies against human antibody subclasses (Southern Biotech). Secondary incubations were performed over 2 hours ...
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bioRxiv - Immunology 2021Quote: ... Binding of primary antibodies was then measured via HRP-tagged antibodies specific for the relevant species (SouthernBiotech) and visualized by chemiluminescent substrate (Immobilon Classico/ Forte western HRP substrate or Thermo Fisher SuperSignal West Dura Extended Duration Substrate).
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bioRxiv - Microbiology 2024Quote: ... Antibody subtype identification was performed using the SBA ClonotypingTM System/HRP Kit (Southern Biotech, Birmingham, AL, USA). To minimize the background impurities observed during immunoelectron microscopy ...
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bioRxiv - Cell Biology 2024Quote: ... or HRP-conjugated α-mouse IgM (Southern Biotech, 1020-05, 1:5000 in 1 % BSA in PBS) for 4 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and bound biotinylated IgG was detected using horseradish peroxidase-conjugated streptavidin (Southern Biotech, Birmingham, AL) (1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... blocked with PBS containing 10% (v/v) Normal Goat Serum (SouthernBiotech, 0060-01) for 1 h and incubated in primary antibodies overnight at 4°C in blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... blocked with PBS containing 10% (v/v) Normal Goat Serum (SouthernBiotech, 0060-01) for 1 hour and incubated in primary antibodies overnight at 4°C in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... blocked with PBS containing 10% (v/v) normal goat serum (SouthernBiotech, 0060-01) for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... followed by α-Rabbit (4050-05, Southern Biotech) or α-Mouse (1031-05 ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Immunology 2023Quote: ... + Human IgM-L (cat# 0158L-01, southern biotech) + Human IgA-k (cat# 0155K-01, Southern Biotech). Goat anti-human IgM-AP (cat# 2020-04 ...
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bioRxiv - Cell Biology 2023Quote: ... The coverslips were mounted on poly-L-lysine-coated slides using DAPI-Fluoromount G (Southern Biotech). Imaging was as described for fluorescence microscopy above ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with PBSTw and incubated for three hours with appropriate HRP-conjugated secondary antibody (SouthernBiotech) at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and rabbit 4050-05) were purchased from Southern Biotech. Secondary antibodies for immunofluorescence (Alexa Fluor 546 goat anti-mouse IgG1 A21123 ...
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bioRxiv - Microbiology 2020Quote: ... Antibody concentration was determined by comparing absorbance values to known concentrations of mouse IgG (0107-01, SouthernBiotech) bound to the plate with goat anti-mouse Ig (1010-01 ...
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bioRxiv - Immunology 2021Quote: ... For PBS suspensions of feces and mouse IgA and IgG (Southern Biotech, Cat#0106-01; RRID: AB_2714214), 5 × 105 ler/lux-expressing C ...
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bioRxiv - Immunology 2024Quote: ... Spots were developed using α-mouse IgG or α-mouse IgA alkaline phosphatase (AP) Abs (Southern Biotech) followed by BCIP/NBT AP substrate (Vectors Labs) ...
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bioRxiv - Immunology 2021Quote: ... the same ELISA was performed but using the secondary antibodies from SBA Clonotyping System-HRP kit (SouthernBiotech, 5300-05, RRID:AB_2796080) diluted accordingly to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The ELISA plates were then washed four times with PBS and incubated with HRP-conjugated mouse IgG1- or IgG2c-specific Abs (SouthernBiotech) for 1 h at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue sections were blocked with 10% goat serum and 0.1% Triton X-100 (Southern Biotech) for 1 h at room temperature and then incubated with anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were blocked with 10% goat serum and 0.1% Triton X-100 (Southern Biotech) for 1 h at room temperature and then incubated with anti-MBP (Abcam ...
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bioRxiv - Immunology 2021Quote: ... To generate a standard curve a dilution series using the Monkey IgG-UNLB antibody (Southern Biotech, 0135-01) was coated on each plate and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01 ...
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bioRxiv - Biochemistry 2023Quote: ... or a 5-fold dilution series starting at 200ng/ml of purified IgG (Catalog #0107-01, Southern Biotech) or IgA (Catalog #0106-01 ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed 4x with PBS-T and incubated for 1h at room tepmerature either with HRP-IgG1 (SouthernBiotech 1070-05) (1:4,000 ...
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bioRxiv - Bioengineering 2023Quote: ... The procedure was carried out as follows: (1) block sections in 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:1000 in PBST in order to detect the primary antibody for 1 h at room temperature before being mounted on glass slides and coverslipped with Fluoromount-G (0100-01, Southern Biotech). Images of cresyl violet stained sections were obtained using an Axio Observer Carl Zeiss light microscope whereas fluorescent images from immunolabelled sections were collected using an Axio Observer Carl Zeiss fluorescent microscope ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACs buffer (1% BSA, 1% Na+azide) and secondary antibody IgG-PE (Southern Biotech, Alabama, USA) was added in a 1:200 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... The tissues were washed with PBT and rinsed with PBS and then the wing imaginal discs were mounted on poly-L-lysine-coated coverslips using Fluoromount-G (SouthernBiotech). Tissues were imaged using either a Leica LSM 510 or a Nikon C1 upright confocal microscope and processed using ImageJ (version 2.0 ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Microbiology 2024Quote: ... Concentrations were evaluated by linear regression analysis built by potting OD405 values against a standard curve generated by titration of a human IgG-unlabeled antibody (Southern Biotech).
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bioRxiv - Immunology 2019Quote: ... The bound antibodies from fecal supernatants were detected with goat antimouse IgA that was conjugated with alkaline phosphatase (SouthernBiotech), followed by development with TMB substrate (Bioshop).
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bioRxiv - Immunology 2022Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Immunology 2023Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Microbiology 2023Quote: ... cells were attached to a poly-L-lysine treated slide before 5 μl of Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech) was added and coverslip applied ...
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bioRxiv - Bioengineering 2023Quote: ... The staining procedure involved the following steps: (1) The sections were blocked in a solution of 10% normal goat serum (SouthernBiotech) and 0.3% Triton-X in PBS for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... PE anti-IgA (SouthernBiotech). For biotinylated primary antibodies ...
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bioRxiv - Immunology 2024Quote: ... or anti-human (SouthernBiotech) (1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01; IgA, SouthernBiotech, Cat # 0155K-01) at known concentrations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2019Quote: ... The arrays were washed 3x 300 μL/well on the plate washer before adding 100 μL/well of a 1/5000 cocktail of biotinylated secondary polyclonal goat antibodies (Southern Biotech) directed to the human kappa and lambda light chains in assay diluent ...
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bioRxiv - Neuroscience 2020Quote: ... All coverslips were then washed three times (five minutes per wash) in 1xPBS followed by block and permeabilization with 5% (v/v) normal goat serum (NGS, Southern Biotech) and 0.25% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... localization of antibody labeling was detected after a 1 hr incubation with secondary antibody diluted in PBS containing 2% normal goat serum and mounting under a coverslip with DAPI Fluoromount-G (Southern Biotech). PPCs were visualized by fluorescent microscopy and their numbers were assessed qualitatively by visual examination ...