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Citations for SouthernBiotech :
651 - 699 of 699 citations for Killer cell immunoglobulin like receptor 2DL2 KIR2DL2 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with 1x PBS and incubated with 1:4000 anti-human IgG-HRP (Southern Biotech) for 1h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and coverslips were mounted on top of the cells using Fluoromount-G mounting solution containing DAPI (Southern Biotech). Single slice images were taken on a Zeiss 880 microscope using the confocal settings.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed a further three times before mounting with Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... cell nuclei were stained using DAPI during the first round of HCR-FISH staining (DAPI Fluoromount-G; SouthernBiotech).
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely screened and confirmed negative for mycoplasma contamination at least every three months (SouthernBiotech #13100-01).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Immunology 2024Quote: ... Human B cell stimulation used 10ng/mL Goat F(ab’)2 Anti-human IgM (Southern Biotech #2022-01), 2ug/mL of recombinant human sCD40 (invitrogen PHP0025) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 times with PBST and mounted on slides with Fluoromount-G (SouthernBiotech #0100-01) or ProLong™ Glass Antifade Mountant (Invitrogen P36984) ...
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bioRxiv - Immunology 2023Quote: ... cover slips with stained cells were mounted with DAPI Fluoromount-G (Southern Biotech, AL, USA, Cat. 0100-20) on microscope slides and analyzed by an AxioObserver Z.1 Microscope with 40x or 63x objectives (Zeiss ...
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were counter-stained with DAPI for 5 min and then mounted with anti-fade Fluoromount-G (SouthernBiotech) for further observation under a fluorescence microscope (Leica DMi8).
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bioRxiv - Pathology 2024Quote: ... Cells were then washed in PBS and slides were mounted in Fluoromount mounting medium (Southern Biotech, Birmingham, USA) for the microscopy analysis.
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bioRxiv - Cancer Biology 2024Quote: ... Fluoromount-G was used to mount cells for imaging and was obtained from Southern Biotech (Cat# 0100-01). Glutathione sepharose beads used for the Ral activity assay were from GE (Cat# 17075601) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells lines were authenticated and tested for mycoplasma contamination before use via the Mycoplasma Detection Kit (SouthernBiotech; 13100-01). Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Neuroscience 2020Quote: ... the cover slips with cultured cortical cells or slides with brain tissue were either mounted with Fluoromount-G (SouthernBiotech) or subjected to a subsequent immunocytochemistry ...
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bioRxiv - Physiology 2020Quote: ... Cells were also regularly checked for mycoplasma contamination using a PCR based mycoplasma detection kit from SouthernBiotech (Birmingham, AL) and were confirmed to be negative ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Microbiology 2023Quote: ... The cell nuclei were labeled with a DAPI stain present in the mounting media (DAPI Fluoromount-G, Southern Biotech). Images were acquired using an upright microscope (Echo Revolve ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times in PBS and mounted on glass slides using Fluoromount G (Southern Biotech; 0100-01). Prepared specimens were imaged on a Leica DMi8 (LAS X software ...
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bioRxiv - Immunology 2020Quote: ... 4 single cell clones of DT40 WT or Fam72a−/− were stained with anti-IgM-PE (1:40, clone M-1, SouthernBiotech) and 12 populations of 5×104 IgM− cells were sorted for each clone ...
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bioRxiv - Immunology 2021Quote: ... the pellet was resuspended in a 50 μL mixture of PE-anti-human CD11b (Southern Biotech, 10 μL/million cells), Alexa Fluor 647-anti-human CD35 (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on glass slides in a 1:1 ratio of YC complete:DAPI Fluoromount-G (SouthernBiotech, Cat #0100-20). Yeast were imaged with a Zeiss 880 LSM with fast Airyscan.
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with DAPI Fluoromount-G (Southern Biotech) to observe intracellular localization using an Olympus BX63 fluorescence microscope ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3x with DPBS and coverslips mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, #0100-01). Cells were imaged using a Zeiss Axio Observer 7 fitted with a 40x objective using DAPI and Alexa Fluor 488 filters ...
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bioRxiv - Cell Biology 2023Quote: ... Cells cultured on glass coverslips were then lifted and mounted on microscope slides using Fluoromount-G Mounting Medium (Southern Biotech) and stored at 4C overnight ...
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bioRxiv - Immunology 2024Quote: ... Anti-human IgM F(ab’)2 (Jackson Immuno) for the IgM Ramos cell lines or Anti-human IgG F(ab’)2 (SouthernBiotech) for the IgG cell lines was also included in the antigen plate ...
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bioRxiv - Immunology 2021Quote: ... and double-distilled water to remove cells before being incubated with alkaline phosphatase-conjugated anti-mouse IgM or anti-mouse IgG1 (Southern Biotech) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were tryprinized and resuspended in binding buffer at 72 hours post-transfection and incubated with Annexin V-FITC (Southern Biotech) and propidium iodide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Labeled cells were washed three times with TBS before being covered with Fluoromount mounting medium (Southern Biotech, Biozol Diagnostica, Eching, Germany). Photos were taken using confocal laser scanning microscopy (TCS SPEII ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with 50 μL of the secondary mix (goat anti-human IgG-AF488 (SouthernBiotech, cat. #2040-30) and goat anti-mouse Alexa Fluor 488 IgG (H + L ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were authenticated every 6 – 12 months through STR profiling at MD Anderson’s Cytogenetics and Cell Authentication Core and were routinely checked for mycoplasma (SouthernBiotech #13100-01). Cen3tel cells were authenticated as having distinct STR profiles from all other cell lines and being similar to each other across PDs ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3x with DPBS and coverslips were mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, 0100-01).
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with Fluoromount-G (Southern Biotech 0100-01) for image acquisition ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The cells were rinsed three times in 1xPBS and mounted on slides using Fluoromount-G Slide Mounting Medium (Southern Biotech, 0100-01). Slides were images on a Zeiss Axio Observer Z1 microscope coupled to the Perkin Elmer UltraVIEW Vox spinning disk confocal microscopy system ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed three times with PBST for 15 minutes each and mounted with DAPI Fluoromount G (SouthernBiotech, Cat# 0100-20) for imaging.
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bioRxiv - Microbiology 2023Quote: ... cells were attached to a poly-L-lysine treated slide before 5 μl of Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech) was added and coverslip applied ...
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bioRxiv - Biophysics 2021Quote: ... We washed the cells with wash solution and added 50 µl of mounting solution (DAPI Fluoromount-G, 0100-20, Southern Biotech, Birmingham, AL). Finally ...
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bioRxiv - Immunology 2022Quote: ... and the cells were stained with 50 μL/well of a 1:1,000 dilution of goat anti-human IgG-PE (Southern Biotech, cat. # 2040-09) secondary antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... primary B cells (107/ml) were stained for 5 min on ice with 10 μg/ml of biotinylated anti-IgM (Southern Biotech, 1021-08) and washed with Imaging Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... China).The apoptosis rate of U251 cells inoculated in the lower compartment was measured using a membrane-bound V-PE/7AAD kit (Southern Biotech, Birmingham, Ala, USA) after 12h.Then washing with phosphate buffer and centrifugation twice ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the cells were stained with DAPI solution and washed three times before mounting with Fluoromount-G® Anti-Fade Mounting Medium (Southern Biotech, Birmingham, AL). Images were captured using Zeiss Observer Z1 confocal microscope (Carl Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were incubated for 60 min in blocking buffer (TBS-LI-COR, Biosciences #927-60001; supplemented with 1% Goat Serum, Southern Biotech #00060-01). Fixed cells were then incubated with primary (ON ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with Fc stain buffer and stained with Alexa Fluor 647 goat anti-human IgG (Southern Biotech [Birmingham, AL], cat. 2016-31) in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend ...
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bioRxiv - Genetics 2024Quote: ... The following antibody mixtures were used for B cell (FITC rat anti-mouse CD43, BD Pharmingen 553270; PE goat anti-mouse IgM, Southern Biotech 1020-09; PE-Cy5 anti-Hu/Mo CD45R (B220), eBioscience 15-0452-83 ...